Characterization of slow-gelling alginate hydrogels for intervertebral disc tissue-engineering applications

Kalaf, Emily A. Growney; Flores, Reynaldo; Bledsoe, J. Gary; Sell, Scott A.

doi:10.1016/j.msec.2016.02.067

Cited by 72 publications

(39 citation statements)

References 33 publications

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“…To overcome these limitations, a number of injectable systems such as bulk hydrogels and porous microbeads have been proposed and investigated . Minimally invasive delivery strategies are preferred in a wide range of medical scenarios including dermatology, urology, gastroenterology, orthopedics, dentistry, etc., as they allow quick delivery, ease of application, and an increase in patient comfort over traditional surgeries.…”

Section: Introductionmentioning

confidence: 99%

Electric Field Assisted Microfluidic Platform for Generation of Tailorable Porous Microbeads as Cell Carriers for Tissue Engineering

Costantini

Guzowski

Żuk

et al. 2018

Adv Funct Materials

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Injection of cell-laden scaffolds in the form of mesoscopic particles directly to the site of treatment is one of the most promising approaches to tissue regeneration. Here, a novel and highly efficient method is presented for preparation of porous microbeads of tailorable dimensions (in the range ≈300-1500 mm) and with a uniform and fully interconnected internal porous texture. The method starts with generation of a monodisperse oil-in-water emulsion inside a flow-focusing microfluidic device. This emulsion is later broken-up, with the use of electric field, into mesoscopic double droplets, that in turn serve as a template for the porous microbeads. By tuning the amplitude and frequency of the electric pulses, the template droplets and the resulting porous bead scaffolds are precisely produced. Furthermore, a model of pulsed electrodripping is proposed that predicts the size of the template droplets as a function of the applied voltage. To prove the potential of the porous microbeads as cell carries, they are tested with human mesenchymal stem cells and hepatic cells, with their viability and degree of microbead colonization being monitored. Finally, the presented porous microbeads are benchmarked against conventional microparticles with nonhomogenous internal texture, revealing their superior performance.

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Section: Introductionmentioning

confidence: 99%

Electric Field Assisted Microfluidic Platform for Generation of Tailorable Porous Microbeads as Cell Carriers for Tissue Engineering

Costantini

Guzowski

Żuk

et al. 2018

Adv Funct Materials

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“…Cyclic loading (Figure c,d) shows no significant difference in percent change hysteresis over any alginate over time with the exception of PRP‐encapsulated alginates at day 14, indicating greater crosslinking stability of the functionalized alginates over encapsulated alginates. The alginates are subjected to the same concentrations of crosslinkers as it has been shown previously that energy loss upon cyclic loading may be more dependent upon crosslinking than the material (Growney Kalaf et al, ). Significantly higher stress relaxation is exhibited in the functionalized alginates over encapsulated on day 4 ( p < .0183), and by day 14 the encapsulated alginates trend toward an increased change in hysteresis as well as stress relaxation over both functionalized and control alginates.…”

Section: Resultsmentioning

confidence: 99%

Bio‐conjugation of platelet‐rich plasma and alginate through carbodiimide chemistry for injectable hydrogel therapies

et al. 2019

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Alginate is a highly tailorable, biocompatible polymer whose properties can be tuned to mimic the properties of native nucleus pulposus (NP) tissue. Platelet-rich plasma (PRP) is a highly accessible, inexpensive, and readily available mix of pro-regenerative factors. By functionalizing alginate with PRP, a mechanically optimized, bioactive alginate NP analogue may stimulate NP cells to proliferate and accumulate matrix over a longer period of time than if the PRP were solely encapsulated within the hydrogel.In this study, PRP was chemically bound to alginate using carbodiimide chemistry and mechanically, physically, and cytologically compared to plain alginate as well as alginate containing free-floating lyophilized PRP. The alginates were mechanically and physically characterized; PRP-conjugated alginate had similar mechanical properties to controls and had the benefit of retained PRP proteins within the hydrogel. Human nucleus pulposus cells (hNPCs) were seeded within the modified alginates and cultured for 14 days. Quantification data of glycosaminoglycans suggests that PRPincorporated alginate has the potential to increase ECM production within the characterized alginate constructs, and that PRP-functionalized alginate can retain protein within the hydrogel over time. This is the first study to functionalize the milieu of PRP proteins onto alginate and characterize the mechanical and physical properties of the modified alginates. This study also incorporates hNPCs into the characterized PRP-modified alginates to observe phenotypic maintenance when encapsulated within the in situ gelling constructs.

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“…As it is seen in the literature, experimental set‐ups are generally established using animal tissues as live mammalian subjects in the studies of toxicity or cytotoxicity. As it is known that the sensitivity of human and animal tissues is different, therefore, the results obtained can be different and misleading as well . In many studies where animal tissues are not used, commercial cell lines are used …”

Section: Discussionmentioning

confidence: 99%

Are radio‐contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells?

Karaarslan

Yılmaz

Özbek

et al. 2018

Basic Clin Pharma Tox

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In the literature, there have been no studies showing clear results on how radio-contrast pharmaceuticals would affect intact disc tissue cells. In this context, it was aimed to evaluate the effects of iopromide and gadoxetic acid, frequently used in the discography, on intact lumbar disc tissue in pharmaco-molecular and histopathological level. Primary cell cultures were prepared from the healthy disc tissue of the patients operated in the neurosurgery clinic. Except for the control group, the cultures were incubated with the indicated radio-contrast agents. Cell viability, toxicity and proliferation indices were tested at specific time intervals. The cell viability was quantitatively analysed. It was also visually rechecked under a fluorescence microscope with acridine orange/propidium iodide staining. Simultaneously, cell surface morphology was analysed with an inverted light microscope, while haematoxylin and eosin (H&E) staining methodology was used in the histopathological evaluations. The obtained data were evaluated statistically. Unlike the literature, iopromide or gadoxetic acid did not have any adverse effects on the cell viability, proliferation and toxicity (P < 0.05). Although this study reveals that radio-contrast pharmaceuticals used in the discography, often used in neurosurgical practice, can be safely used, it should be remembered that this study was performed in an in vitro environment.

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Characterization of slow-gelling alginate hydrogels for intervertebral disc tissue-engineering applications

Cited by 72 publications

References 33 publications

Electric Field Assisted Microfluidic Platform for Generation of Tailorable Porous Microbeads as Cell Carriers for Tissue Engineering

Electric Field Assisted Microfluidic Platform for Generation of Tailorable Porous Microbeads as Cell Carriers for Tissue Engineering

Bio‐conjugation of platelet‐rich plasma and alginate through carbodiimide chemistry for injectable hydrogel therapies

Are radio‐contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells?

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