1985
DOI: 10.1016/0003-9861(85)90611-3
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Characterization of skeletal muscle insulin receptor

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1986
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Cited by 11 publications
(2 citation statements)
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“…Tyrosine phosphorylation was detected with antiphosphotyrosine-horseradish peroxidase conjugates (PY-20). To determine the effects of gangliosides on IR phosphorylation, IR was partially purified from skeletal muscle of C57BL/6 mice at 4°C by a modification of the methods of Dubler et al (21). Briefly, the particulate fractions of homogenates, prepared in 50 mM HEPES, pH 7.5, 8% sucrose, 10 mM EDTA, 10 mM EGTA, 1 mM phenylmethylsulfonyl fluoride, 10 M leupeptin, and 1 M pepstatin A, were solubilized with 2% Triton X-100 and further purified by sequential column chromatography on Sephacryl S-200 and wheat germ agglutinin-agarose.…”
Section: Methodsmentioning
confidence: 99%
“…Tyrosine phosphorylation was detected with antiphosphotyrosine-horseradish peroxidase conjugates (PY-20). To determine the effects of gangliosides on IR phosphorylation, IR was partially purified from skeletal muscle of C57BL/6 mice at 4°C by a modification of the methods of Dubler et al (21). Briefly, the particulate fractions of homogenates, prepared in 50 mM HEPES, pH 7.5, 8% sucrose, 10 mM EDTA, 10 mM EGTA, 1 mM phenylmethylsulfonyl fluoride, 10 M leupeptin, and 1 M pepstatin A, were solubilized with 2% Triton X-100 and further purified by sequential column chromatography on Sephacryl S-200 and wheat germ agglutinin-agarose.…”
Section: Methodsmentioning
confidence: 99%
“…Of these, skeletal muscle represents the largest target, making up -2 5 -4 5 % of body weight in men (2). Although insulin receptors from this tissue have been identified and characterized (3,4), relatively few studies have been performed on the receptor kinase of skeletal muscle, largely due to technical difficulties in isolating the receptor.…”
mentioning
confidence: 99%