Characterization of six small HSP genes from Chironomus riparius (Diptera, Chironomidae): Differential expression under conditions of normal growth and heat-induced stress

Martín-Folgar, Raquel; Fuente, M. de la; Morcillo, Gloria; Martínez-Guitarte, José-Luis

doi:10.1016/j.cbpa.2015.06.023

Cited by 42 publications

(23 citation statements)

References 63 publications

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“…XP_020704257), with an ACD between 51‐140 aa. Moreover, Chironomus riparius (commonly known as the harlequin fly) sequence was 817 bp in length and its ORF coded for a protein of 148 aa, confirming the overall results of our protein identification.…”

Section: Resultssupporting

confidence: 83%

Small heat shock protein genes of the green algae Closterium ehrenbergii: Cloning and differential expression under heat and heavy metal stresses

Abassi

Wang

Ponmani

et al. 2019

Environmental Toxicology

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The freshwater green algae Closterium ehrenbergii has been considered as a model for eco‐toxicological assessment in aquatic systems. Heat shock proteins (HSPs) are a class of highly conserved proteins produced in all living organisms, which participate in environmental stress responses. In the present study, we determined the cDNA sequences of small heat shock protein 10 (sHSP10) and sHSP17.1 from C. ehrenbergii, and examined the physiological changes and transcriptional responses of the genes after exposure to thermal shock and toxicants treatments. The open reading frame (ORF) of CeHSP10 was 300 bp long, encoding 99 amino acid (aa) residues (10.53 kDa) with a GroES chaperonin conserved site of 22 aa. The CeHSP17.1 had a 468 bp ORF, encoding 155 aa with a conserved C‐terminal α‐crystallin domain. For heat stress, cells presented pigment loss and possible chloroplast damage, with an up‐regulation in the expression of both sHSP10 and sHSP17.1 genes. As for the heavy metal stressors, an increase in the production of reactive oxygen species was registered in a dose dependent manner, with a significant up‐regulation of both sHSP10 and sHSP17.1 genes. These results suggest that sHSP genes in C. ehrenbergii may play a role in responses to stress environments, and they could be used as an early detection parameter as biomarker genes in molecular toxicity assessments.

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Section: Resultssupporting

confidence: 83%

Small heat shock protein genes of the green algae Closterium ehrenbergii: Cloning and differential expression under heat and heavy metal stresses

Abassi

Wang

Ponmani

et al. 2019

Environmental Toxicology

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“…Here, α‐crystallin domain of the eight sHSPs consists of approximately 100 amino acid residues and nine common β‐strands, with an elongated β6 + β7 strand that is inferred to be at the dimer interface, like human αB‐crystallin (Bagneris et al., ). The conservative α‐crystallin domain shows a high identity of 72.6%, and in the same position, there contains six putative dimer interface (shown as red boxes in Figure ), which is involved in the stabilization and solubilization of the oligomeric assemblies (Martin‐Folgar et al., ). Even in the region of the variable N‐terminal, which also contains the target‐protein binding sites and plays an important role in the organization of large sHSP oligomers (Sun & MacRae, ), there still possess 36.2% identity among these eight sHSPs.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, it was inferred that these orthologous sHSP genes are house‐keeping genes and are constitutively expressed in organisms due to their importance in the development, adaptability and metabolism of organisms (Li et al., ). While acting as molecular chaperones to assist a wide range of folding processes during the insects development, they are also considered not to be stress‐inducible (Feder & Hofmann, ; Martin‐Folgar et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Insects, the most diverse and ecologically dominant animals on land, are suitable model organisms for the investigation of potential mechanisms underlying induced and intrinsic thermo-tolerance (Li, Moghaddam, Du, Zhong, & Chen, 2012), several studies also have discussed the functions of shsps in insects, as well as developed comparative analysis of their homologous relationship (Li et al, 2009;Martin-Folgar, de la Fuente, Morcillo, & Martinez-Guitarte, 2015;Sakano et al, 2006). Phylogenetic analysis showed that only one orthologous cluster of sHSPs exists among B. mori, D. melanogaster, Anopheles gambiae, Apis mellifera and Tribolium castaneum, and the others have evolved independently among different insect orders (Li et al, 2009).…”

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confidence: 99%

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Characterization and functional analysis of hsp21.8b: An orthologous small heat shock protein gene in Tribolium castaneum

Xie

Xiong

et al. 2018

J Applied Entomology

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Small heat shock proteins (sHSPs) act as molecular chaperones and are widely distributed in all kinds of organisms. Comparative analysis revealed that an orthologous shsp was present during insect evolution. Here, hsp21.8b, one insect orthologous shsp, had been identified in Tribolium castaneum. Quantitative real‐time PCR illustrated that Tchsp21.8b was expressed in all developmental stages, along with the lowest expression at early embryonic stage and relative high expression at other stages especially in late eggs and late pupae. In the adult period, Tchsp21.8b exhibited the highest expression level in central nervous system and followed in elytron, epidermis, ovary and fat body. Moreover, it was upregulated 3.39‐fold in response to enhanced heat stress (45°C) for 4 hr but not to cold stress (4°C) and was upregulated by 1.73‐ to 1.94‐fold under ultraviolet (UV) exposure during 4–6 hr. It was also downregulated by 20.8%–41.8% under starvation in 3 days and had a “down‐up‐down” trend under the pathogen stresses. Larval RNA interference of Tchsp21.8b caused 40.6% insects mortality and reduced the oviposition amount by 66.0% and only 21.0% of the ds‐Tchsp21.8b eggs could hatch into larvae. These results suggested that as an orthologous shsp, Tchsp21.8b not only plays important roles in the growth, development and fecundity of T. castaneum but with the competence to resist the environment stresses, although the response is relatively weak compared to other hsps. Results from this study also uncovered the functions of the orthologous shsp in the development and anti‐stresses ability of T. castanuem. It provided more scientific evidence for revealing the physiological mechanisms of shsps of the insects and enhanced the capabilities to control different pests.

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“…Heterogeneity of sHSPs is unique to plants as plants are only eukaryotes in which organelle localized sHSPs have been described with the exception of mitochondria localized sHSPs in animal cells (Lee et al 2007). This heterogeneity of sHSPs to plants suggested that they might have unique physiological functions (Martín-Folgar et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of nine different small heat shock protein gene promoters in Oryza sativa L. subsp. indica

et al. 2016

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Small heat shock proteins (sHSPs), the intra cellular chaperons respond to high temperatures and other stresses, are expressed under the control of the heat shock factors, which recognize highly conserved cis-regulatory elements (CREs). The present study was designed to identify and compare nine different sHSP gene promoters from rice on the basis of their location on chromosomes, sequence similarities, evolutionary relationship and putative binding sites of transcription factors. Additionally, they were analyzed for their functional importance in the regulation of sHSP genes. For this, 5 0 upstream region of nine sHSP genes from Oryza sativa was amplified, sequenced and analyzed. RFLP mapping approach using molecular linkage maps of three-way cross-population showed that OsHSP 16.9A, OsHSP 16.9B, OsHSP 16.9C and OsHSP C-II genes were clustered on the short arm of chromosome 1 with a 1238-bp overlapped sequence between OsHSP 16.9A and OsHSP 16.9B, whereas OsHSP 17.3, OsHSP 17.7, OsHSP 17.9, OsHSP 18.0 and OsHSP 26 genes were clustered on chromosome 3 with a 420-bp overlapped sequence between OsHSP 17.3 and OsHSP 18.0. Evolutionary analysis revealed that sHSPs that clustered on different chromosomes in the same specie showed high sequence similarity and evolved prior to the divergence of their localization. Eighteen CREs remained conserved among all sHSP gene promoters during evolution. These common regulatory elements are related to disease resistance, hormonal responses, light responses, seed storage proteins, tissue-specific expression, pollen development and other environmental stresses. Moreover, CCAATBOX1 was also found in all sHSP gene promoters involved in the regulation of heat shock response. Conclusively, promoter region of sHSP genes seems to be capable of driving genes expression during stress and genetic manipulation might be helpful in producing better quality rice.

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Characterization of six small HSP genes from Chironomus riparius (Diptera, Chironomidae): Differential expression under conditions of normal growth and heat-induced stress

Cited by 42 publications

References 63 publications

Small heat shock protein genes of the green algae Closterium ehrenbergii: Cloning and differential expression under heat and heavy metal stresses

Small heat shock protein genes of the green algae Closterium ehrenbergii: Cloning and differential expression under heat and heavy metal stresses

Characterization and functional analysis of hsp21.8b: An orthologous small heat shock protein gene in Tribolium castaneum

Comparative analysis of nine different small heat shock protein gene promoters in Oryza sativa L. subsp. indica

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