Characterization of sheep (Ovis aries) palatine tonsil innervation

Russo, Domenico; Fantaguzzi, C. Mongardi; Guardo, Giovanni Di; Clavenzani, Paolo; Costerbosa, Giovanna Lalatta; Ligios, Ciriaco; Chiocchetti, Roberto

doi:10.1016/j.neuroscience.2009.04.015

Cited by 9 publications

(4 citation statements)

References 42 publications

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“…During postmortem examination, the brain and spinal cord, together with the ganglia innervating the ovine PTs (20), namely, the cervical cranial ganglia (CCG), proximal glossopharyngeal ganglia (PGG), proximal vagal ganglia (PVG), distal vagal ganglia (DVG), trigeminal ganglia (TG), and cervicothoracic ganglia (CTG), were adequately sampled. The gut-associated lymphoid tissue, including PTs, along with several lymph nodes, were also promptly collected (Tables 1, 2, and 3).…”

Section: Methodsmentioning

confidence: 99%

Role of Palatine Tonsils as a Prion Entry Site in Classical and Atypical Experimental Sheep Scrapie

Chiocchetti

et al. 2014

J Virol

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Section: Methodsmentioning

confidence: 99%

Role of Palatine Tonsils as a Prion Entry Site in Classical and Atypical Experimental Sheep Scrapie

Chiocchetti

et al. 2014

J Virol

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“…(2006), for SP by Pfannkuche et al. (2002), for TH and DBH by Russo et al. (2009), and for IB4 by Mazzoni et al.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, in past studies (Lalatta-Costerbosa et al 2007) and in the present study we found a total overlapping between histochemical and immunohistochemical stained enteric neurons. For CGRP, the specificity was tested by Chiocchetti et al (2006), for SP by Pfannkuche et al (2002), for TH and DBH by Russo et al (2009), and for IB4 by Mazzoni et al (2010). The specificity of the CALB and GAL antibodies has previously been published (Dunning et al 1986;Reiche et al 1999).…”

Section: Quantitative and Statistical Analysismentioning

confidence: 99%

An immunohistochemical study of the distribution of nitric oxide synthase-immunoreactive neurons and fibers in the reticular groove of suckling lambs

Lalatta-Costerbosa¹,

Clavenzani²,

Petrosino³

et al. 2011

Journal of Anatomy

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The reticular groove (RG) is a specialized region of ruminant forestomach which, in suckling animals, via a vagovagal reflex, transforms itself into a tube to ensure the direct transport of milk from the esophagus to the abomasum. The nervous mechanism controlling the RG movement is not fully understood; however, at this level, the enteric nervous system (ENS) shows the highest neuronal density when compared with other forestomach compartments. Because nitric oxide is considered the putative major mediator of non-adrenergic noncholinergic smooth muscle relaxation, the aim of the present study was to investigate the ENS of the RG of suckling lambs, both in the floor and in the lip, with particular regard to nitric oxide synthase (NOS)-immunoreactivity (-IR), by means of double immunohistochemical staining. NOS antiserum was used in association with some neurochemical markers which have been utilized by many authors in ENS. A rich innervation of fibers extended along the entire length of the RG. Proceeding distally, the number of neurons stained with a panneuronal marker increased; they were more numerous in the lips and lip-floor junction than in the floor itself. However, the percentage of NOS-IR neurons was the same in the proximal and distal parts. Many NOS-IR neurons often co-expressed galanin and dopamine b-hydroxylase. Neurochemical markers, such as calbindin, calcitonin gene-related peptide, IB4 and neurofilament 200 kDa, usually used to identify primary sensory neurons were not expressed in RG neurons, and the co-localization of NOS with tyrosine hydroxylase and substance P was rarely found. When compared with other districts, the RG showed some peculiar aspects, such as the lack of both neurons in the submucosal plexus and the lack of typical sensory neurons.

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“…To aid the unravelling of the pathogenesis of transmissible spongiform encephalopathies (TSEs), morphological and immunological data on the five ovine tonsils, including the paired palatine tonsil [tonsilla (t.) palatina] located in the oropharynx, the paired paraepiglottic tonsil (t. paraepiglottica) in the laryngopharynx, and the tonsil of the soft palate (t. veli palatina), the pharyngeal tonsil (t. pharyngea) and the paired tubal tonsils (t. tubaria) in the nasopharynx (Casteleyn et al., , ; Breugelmans et al., ), were gathered. The exact anatomical localizations, histological characteristics, volumes and surface areas, distribution of lymphoid cell populations and innervation of the ovine tonsils have yet been described (Casteleyn et al., , , ; Russo et al., ; Breugelmans et al., ,b,c; Toppets et al., ).…”

Section: Introductionmentioning

confidence: 99%

Lymph Drainage from the Ovine Tonsils: An Anatomical Study of the Tonsillar Lymph Vessels

Casteleyn

Cornillie

Ginneken

et al. 2014

Anat. Histol. Embryol.

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Summary Although the tonsils of sheep have gained much attention during the last decade, only few data are available on their lymph vessel architecture. Tonsillar lymph vessels are immunologically important as they form the efferent routes for locally activated immune cells to reach the draining lymph nodes. To gain insight into the tonsillar lymph drainage in the sheep, Indian ink and a casting polymer were injected into the interstitium of the five tonsils present in the heads of slaughtered sheep. This enabled us to determine the draining lymph node and to examine the microscopic organization of lymph vessels using light and scanning electron microscopy. No lymph vessels were observed within the tonsillar lymphoid follicles. The corrosion casts demonstrated that the lymphoid follicles are surrounded by numerous sacculated lymph sinuses that drain into a dense interfollicular lymph vessel network. From here, the lymph flows into single small lymph vessels that in turn drain into larger lymph vessels extending towards the medial retropharyngeal lymph node. The presented results can be valuable for immunological studies, for example during oral or intranasal vaccine development.

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Characterization of sheep (Ovis aries) palatine tonsil innervation

Cited by 9 publications

References 42 publications

Role of Palatine Tonsils as a Prion Entry Site in Classical and Atypical Experimental Sheep Scrapie

Role of Palatine Tonsils as a Prion Entry Site in Classical and Atypical Experimental Sheep Scrapie

An immunohistochemical study of the distribution of nitric oxide synthase-immunoreactive neurons and fibers in the reticular groove of suckling lambs

Lymph Drainage from the Ovine Tonsils: An Anatomical Study of the Tonsillar Lymph Vessels

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