Characterization of sheep milk fat globule proteins by two-dimensional polyacrylamide gel electrophoresis/mass spectrometry and generation of a reference map

Production and Release of Antimicrobial and Immune Defense Proteins by Mammary Epithelial Cells following Streptococcus uberis Infection of Sheep

“…In fact, MFGs originate from the MEC as vesicles released from the apical part of the polarized secretory epithelium and, during this process, carry portions of the lactating cell cytoplasm with them (44). This phenomenon presents with various intensities in different animal species, and it is believed to be more prominent in small ruminants than in bovines (31,32,45,46). In the work presented here, the MFG proteomics approach provided results in line with those obtained with M. agalactiae.…”

“…MFG proteins (MFGPs) were extracted from raw milk as described previously (30)(31)(32). Briefly, milk samples were centrifuged to separate the cream fraction containing MFGs from the remaining protein fraction of milk.…”

Application of proteomics to the areas of milk production, processing and quality control – A review

“…The major proteins of ovine (sheep) skim milk were successfully identified by MALDI‐TOF‐MS, while minor whey spots, such as the MFGM proteins P spots, were subjected to LC‐MS/MS to identify also low abundant proteins (Pisanu et al . ). All the main protein components were identified, and specifically caseins (α s1 ‐CN, α s2 ‐CN, β‐CN and κ‐CN), β‐LG, α‐LA, lactotransferrin, and immunoglobulin alpha heavy and light chain (IGHC and IGLC).…”

“…A mean of 865 spots in the 2‐DE protein profile of sheep MFGM proteins was obtained of which protein spots identified in skimmed milk accounted for a very small percentage (<4%) (Pisanu et al . ). Protein spots chosen among the most abundant and resolved ones were then characterised by LC‐MS/MS.…”

“…A comparison of qualitative data of sheep for MFGM with those available for the cow MFGM proteome showed higher presence of cytoplasmic and secreted proteins in sheep milk ( Pisanu et al . , ) . A mean of 865 spots in the 2‐DE protein profile of sheep MFGM proteins was obtained of which protein spots identified in skimmed milk accounted for a very small percentage (<4%) (Pisanu et al .…”