Characterization of serum protein expression profiles in the early sarcopenia older adults with low grip strength: a cross-sectional study

Abstract

Background Sarcopenia refers to the progressive loss of skeletal muscle mass and muscle function, which seriously threatens the quality of life of the older adults. Therefore, early diagnosis is urgently needed. This study aimed to explore the changes of serum protein profiles in sarcopenia patients through a cross-sectional study, and to provide the reference for clinical diagnosis. Methods This study was a cross-sectional study carried out in the Tianjin institute of physical education teaching experiment training center from December 2019 to December 2020. Ten older adults were recruited, including 5 sarcopenia and 5 healthy older adults. After a detailed diagnostic evaluation, blood samples were collected to prepare serum for proteomic analysis using the HPLC System Easy nLC method. The differentially expressed proteins (DEPs) were screened by the limma package of R software (version 4.1.0). Results A total of 114 DEPs were identified between the patients and healthy older adults, including 48 up-regulated proteins and 66 down-regulated proteins. The functional enrichment analysis showed that the 114 DEPs were significantly enriched in 153 GO terms, which mainly involved in low-density lipoprotein particle remodeling, and negative regulation of immune response,etc. The PPI network further suggested that the cholesteryl ester transfer protein and Apolipoprotein A2 could serve as biomarkers to facilitate diagnosis of sarcopenia. Conclusions This study provided a serum proteomic profile of sarcopenia patients, and identified two proteins with diagnostic value, which might help to improve the diagnostic accuracy of sarcopenia.

Fiber-Type Shifting in Sarcopenia of Old Age: Proteomic Profiling of the Contractile Apparatus of Skeletal Muscles

“…This gives non-invasive biomarker investigations of biological fluids, such as serum, saliva or urine, a central role in aging research [501][502][503]. The serum of both older humans suffering from sarcopenia [504][505][506][507] and senescent mice [508] were shown to exhibit differential changes in common markers that are associated with inflammation, remodeling of the extracellular matrix and mitochondrial functions [398]. This suggests the potential usage of pro-inflammatory cytokines, growth factors, differentiation factors and leaked mitochondrial proteins as suitable biofluid markers to evaluate the degree of skeletal muscle aging [400].…”

Machine Learning Applications in Sarcopenia Detection and Management: A Comprehensive Survey

“…For instance, an increment in plasma asymmetric dimethylarginine (ADMA) level can be measured and is significantly associated with low grip strength and sarcopenia [ 14 ]. In [ 15 ], serum proteomics analysis was used to identify novel biomarkers for the diagnosis of sarcopenia, which found a total of 114 deferentially expressed proteins (DEPs) between the patients and healthy older adults, including 48 upregulated proteins and 66 downregulated proteins, and identified cholesterol ester transfer protein and Apolipoprotein A2 as potential biomarkers that are related to low muscle power.…”