Characterization of Sclerotinia sclerotiorum, an Emerging Fungal Pathogen Causing Blight in Hyacinth Bean (Lablab purpureus)

Prova, Ananya; Akanda, Abdul Mannan; Islam, Shaikhul; Hossain, Md. Motaher

doi:10.5423/ppj.oa.02.2018.0028

Cited by 19 publications

(18 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding characterization of the pathogen S. sclerotiorum, the results of PCR amplification (553-bp product), sequence, and phylogenetic tree of the Egyptian isolate S. sclerotiorum shared with other 15 isolates are in agreement with Prova et al (2018) as they demonstrated that the PCR amplified 564 bp product in the rDNA-ITS region of the fungal S. sclerotiorum isolated from Hyacinth Bean (Lablab purpureus). Also, a consistent trend indicated by several investigators highlights the effectiveness of the nuclear ribosomal internal transcribed spacer (ITS) region sequences located between the nuclear small-and large-subunit rRNA genes in species identification (Prova et al 2018).…”

Section: Discussionsupporting

confidence: 60%

Characterization and potential antifungal activities of three Streptomyces spp. as biocontrol agents against Sclerotinia sclerotiorum (Lib.) de Bary infecting green bean

Gebily

Ghanem

Ragab

et al. 2021

Egypt J Biol Pest Control

View full text Add to dashboard Cite

Background White mold disease, caused by Sclerotinia sclerotiorum the devastating pathogen, attacks green beans (Phaseolus vulgaris L.) and several crops worldwide. The present investigation was carried out to introduce some antagonistic microorganisms as novel antifungal substances to be an alternative and secure method to effectively control the disease. Results Three Streptomyces species, i.e., S. griseus (MT210913 “DG5”), S. rochei (MN700192 “DG4”), and S. sampsonii (MN700191 “DG1”) were isolated, biologically, molecularly characterized, and evaluated in vitro and in vivo. Molecularly, polymerase chain reaction (PCR) amplification and nucleotide sequencing were used to characterize the pathogen and bio-agents. PCR amplification of the pathogen and Streptomyces species (bioagents) exhibited amplicons of around 535 bp and 1300 bp, respectively. The nucleotide sequence analysis of the three Streptomyces spp. indicated that S. rochei was closely related to S. griseus, and both had a distance relationship with S. sampsonii. The evaluation of bioagents was carried out against S. sclerotiorum. Reduction percentages in the mycelial growth of the pathogen ranged between 60.17 and 52.30%, indicating that S. rochie gave the highest inhibition percent. Incorporations of Streptomyces spp. culture filtrate components into culture media proved that S. sampsonii was more efficient as a bioagent in reducing mycelial growth pathogen by 84.50%. The effectiveness of the bioagent volatile compounds inhibited the pathogen growth at a rate of 54.50-72.54%, respectively, revealing that S. rochei was the highest inhibitor followed by S. griseus. The parasitic activity of Streptomyces spp. upon S. sclerotiorum showed deformation, contraction, and collapse when observed by light and scanning electron microscopy. Molecular characterization of the 3 Streptomyces spp. revealed that S. griseus was closely related to S. sampsonii (96%), secondly ranked by S. rochei (93.1%). Viability and germination of pathogen sclerotia were reduced when they dipped into the Streptomyces spore suspensions for 10, 20, and 30 days. Application of the 3 Streptomyces spp. in the field proved a great potential to control the disease. Conclusions The results suggested that the 3 Streptomyces strains and their secondary metabolites can be potential biocontrol agents and biofertilizers for controlling S. sclerotiorum, the causative agent of bean white mold disease.

show abstract

Section: Discussionsupporting

confidence: 60%

Characterization and potential antifungal activities of three Streptomyces spp. as biocontrol agents against Sclerotinia sclerotiorum (Lib.) de Bary infecting green bean

Gebily

Ghanem

Ragab

et al. 2021

Egypt J Biol Pest Control

View full text Add to dashboard Cite

show abstract

“…The external pH has a significant influence on fungal morphogenesis through a highly complex mechanism [42]. Moreover, pH has been a major regulatory factor for procedures linked to the growth, development, pathogenicity, and virulence of S. sclerotiorum [18,39]. This is in accordance with our findings in this study that pH strongly affects mycelial growth and sclerotia development in S. sclerotiorum .…”

Section: Discussionsupporting

confidence: 91%

“…Similarly, sclerotia formation was observed at a temperature between 15°C and 25°C, with an apparent maximum at 20°C. These findings agree that S. sclerotiorum prefers growth in subtropical climates [36,39]. The 20°C optimum reported for growth coincides with the temperatures reported as favoring infection and pathogenesis by S. sclerotiorum [41].…”

Section: Discussionsupporting

confidence: 86%

“…Although S. sclerotiorum has been reported to be the pathogen of the white mold of a pea in other countries [37,38], this is the first documented occurrence to our knowledge of a strain infecting pea in Bangladesh. In Bangladesh, S. sclerotiorum was previously reported on hyacinth bean, jackfruit, and okra [10,17,39,40], implying that the pathogen has the potential to expand to other host species. The broad host range of the pathogen poses an epidemiological threat and makes the management of the disease very challenging.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

First characterization of a newly emerging phytopathogen, Sclerotinia sclerotiorum causing white mold in pea

2021

Self Cite

View full text Add to dashboard Cite

Pea (Pisum sativum L.) is of global importance as a food crop for its edible pod and seed. A new disease causing the tan to light brown blighted stems and pods has occurred in pea (P. sativum L.) plants in Chapainawabganj district, Bangladesh. A fungus with white‐appressed mycelia and large sclerotia was consistently isolated from symptomatic tissues. The fungus formed funnel‐shaped apothecia with sac‐like ascus and endogenously formed ascospores. Healthy pea plants inoculated with the fungus produced typical white mold symptoms. The internal transcribed spacer sequences of the fungus were 100% similar to Sclerotinia sclerotiorum, considering the fungus to be the causative agent of white mold disease in pea, which was the first record in Bangladesh. Mycelial growth and sclerotial development of S. sclerotiorum were favored at 20°C and pH 5.0. Glucose was the best carbon source to support hyphal growth and sclerotia formation. Bavistin and Amistar Top inhibited the radial growth of the fungus completely at the lowest concentration. In planta, foliar application of Amistar Top showed the considerable potential to control the disease at 1.0% concentration until 7 days after spraying, while Bavistin prevented infection significantly until 15 days after spraying. A large majority (70.93%) of genotypes, including tested released pea cultivars, were susceptible, while six genotypes (6.98%) appeared resistant to the disease. These results on identification, characterization, host resistance, and fungicidal control of white mold could be valuable to achieve improved management of a new disease problem for pea cultivation.

show abstract

“…), or oospores ( Phytophthora spp.) [ 7 , 8 , 9 , 10 ]. Microsclerotia and sclerotia have the same anatomical structure, consisting of outer melanized parenchyma cells and inner colorless medullary cells, and are asexual in nature.…”

Section: Introductionmentioning

confidence: 99%

Can Anaerobic Soil Disinfestation (ASD) be a Game Changer in Tropical Agriculture?

Priyashantha

Attanayake

2021

Pathogens

View full text Add to dashboard Cite

Anaerobic soil disinfection (ASD) has been identified as an alternative soil-borne pathogen control strategy to chemical fumigation. ASD involves the application of an easily liable carbon source followed by irrigation to field capacity and maintenance of an anaerobic condition for a certain period. A literature search undertaken on ASD found that more than 50 comprehensive research projects have been conducted since its first discovery in 2000. Most of these studies were conducted in the USA and in the Netherlands. Though the exact mechanism of ASD in pathogen control is unknown, promising results have been reported against a wide range of pathogens such as fungi, nematodes, protists, and oomycetes. However, it is interesting to note that, except for a few studies, ASD research in the developing world and in the tropical countries has lagged behind. Nevertheless, with soil quality depletion, reduction in arable lands, and exponential population growth, a drastic change to the current agricultural practices should be adapted since yield gain has reached a plateau for major staple crops. Under such circumstances, we identified the gaps and the potentials of ASD in tropical agricultural systems and proposed promising biodegradable materials.

show abstract

Characterization of Sclerotinia sclerotiorum, an Emerging Fungal Pathogen Causing Blight in Hyacinth Bean (Lablab purpureus)

Cited by 19 publications

References 36 publications

Characterization and potential antifungal activities of three Streptomyces spp. as biocontrol agents against Sclerotinia sclerotiorum (Lib.) de Bary infecting green bean

Characterization and potential antifungal activities of three Streptomyces spp. as biocontrol agents against Sclerotinia sclerotiorum (Lib.) de Bary infecting green bean

First characterization of a newly emerging phytopathogen, Sclerotinia sclerotiorum causing white mold in pea

Can Anaerobic Soil Disinfestation (ASD) be a Game Changer in Tropical Agriculture?

Contact Info

Product

Resources

About