Characterization of Sclerospora graminicola Isolates from Pearl Millet for Virulence and Genetic Diversity

Pushpavathi, B.; Thakur, R. P.; Rao, K.C.; Rao, V. P.

doi:10.5423/ppj.2006.22.1.028

Cited by 9 publications

(9 citation statements)

References 11 publications

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“…These characteristics of the fungus make it highly variable. Evolution of host-specific virulences in pearl millet downy mildew is well documented (Thakur et al 1992;Sastry et al 2001;Pushpavathi et al 2006b). As a result of evolution of host specific virulences, resistant genotypes lose their effective resistance within a short period which leads to the development of new pathotypes/races in the pathogen populations (Kolmer et al 2006).…”

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confidence: 95%

“…In this context, molecular markers offer numerous genetic features to characterise diverse pathogen populations. DNA markers such as RFLP (Sastry et al 1995), RAPD (Sastry et al 2001) and AFLP (Singru et al 2003;Sivaramakrishnan et al 2003;Pushpavathi et al 2006b) have been used successfully to study the genetic variation in the S. graminicola populations. Apart from elucidation of genetic diversity, molecular markers can also be used to study evolution and monitoring movement/shift of pathogen populations over time and space.…”

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confidence: 99%

“…DNA fingerprinting using AFLP exhibited a high degree of variation in the S. graminicola populations. Variation at the DNA level has been reported in S. graminicola through RAPD (Sastry et al 2001), RFLP (Sastry et al 1995;Sastry et al 1997) and AFLP (Singru et al 2003;Sivaramakrishnan et al 2003;Pushpavathi et al 2006b). This high genotypic variability is because of frequent recombination events taking place in the natural populations of S. graminicola.…”

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Characterisation of pathogenic and molecular diversity inSclerospora graminicola, the causal agent of pearl millet downy mildew

Sharma

Rao

Varshney

et al. 2010

Archives Of Phytopathology And Plant Protection

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Genotypic diversity among 46 isolates of Sclerospora graminicola collected from seven states in India during 1992-2005 was determined through pathotyping and AFLP analysis. A high level of variation was observed among the isolates for downy mildew incidence, latent period and virulence index. Based on the reaction on a set of nine pearl millet lines, 46 isolates were classified in 21 pathotypes. Quantitative differences in virulence levels of the test isolates were assessed by calculating the virulence index (disease incidence 6 latent period 7 1 ). A dendrogram generated by the average linkage cluster analysis of virulence index clustered the 46 isolates into eight groups. Regionspecific grouping of five isolates from Gujarat and six from Rajasthan was observed within two distinct groups. Temporal variation was also observed among the isolates collected from the same location and same host over the years. A total of 297 bands were scored following selective amplification with three primer combinations E-TT/M-CAG, E-AT/M-CAG and E-TG/M-CAT and all of them were polymorphic. Cluster analysis of AFLP data clustered the test isolates into seven groups. Analysis of molecular variance indicated that variation in the S. graminicola populations was largely due to differences among the isolates within the states.

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confidence: 95%

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confidence: 99%

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confidence: 93%

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Characterisation of pathogenic and molecular diversity inSclerospora graminicola, the causal agent of pearl millet downy mildew

Sharma

Rao

Varshney

et al. 2010

Archives Of Phytopathology And Plant Protection

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“…Oospores are the sexual spores, and sporangia and zoospores are the asexual spores produced by the pathogen. Host-specific virulences have also been observed in S. graminicola (Thakur et al, 1992;Sastry et al, 2001;Pushpavathi et al, 2006b). In oomycetes, existence of sexual mating types and their frequency greatly contribute towards the development of new recombinants and, eventually, selection of more virulent populations of the pathogen (Pushpavathi et al, 2006a).…”

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confidence: 99%

New Sources of Resistance to Multiple Pathotypes of Sclerospora graminicola in the Pearl Millet Mini Core Germplasm Collection

et al. 2015

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Downy mildew (DM), caused by Sclerospora graminicola (Sacc.) Schröt., is a highly destructive and widespread disease in most pearl millet [Pennisetum glaucum (L.) R. Br.] growing areas of Asia and Africa. Breeding for DM resistance continues to be an integral part of genetic improvement of pearl millet at ICRISAT, Patancheru, India. For the identification of new and diverse sources of DM resistance, a pearl millet mini core collection comprising 238 accessions was screened against eight pathotypes (Sg 384, Sg 409, Sg 445, Sg 457, Sg 510, Sg 519, Sg 526, and Sg 542) of S. graminicola collected from different geographical locations in India. Significant differences for DM reaction were observed among pathotypes, mini core accessions, and their interactions. Of the 238 accessions, 68 accessions were resistant (≤10% DM incidence) to pathotype Sg 510 followed by 40 accessions resistant to Sg 457. Resistance to pathotypes Sg 519, Sg 526, Sg 384, Sg 445, and Sg 542 was observed in 15, 27, 29, 30, and 34 mini core accessions, respectively. Resistance to two or more pathotypes was observed in 62 accessions. Several of these accessions also exhibited desirable agronomic traits. The multiple‐pathotype‐resistant germplasm accessions having desirable agronomic characteristics and collected from different agro‐ecologies would be useful in breeding programs to develop pearl millet hybrids resistant to difficult‐to‐manage highly‐virulent pathotypes of S. graminicola.

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“…Existence of mating types and their frequency greatly contribute towards the development of new recombinants in the pathogen populations (Pushpavathi et al, 2006a). Evolution of hostspecific virulence in pearl millet downy mildew is well documented (Thakur et al, 1992;Sastry et al, 2001;Pushpavathi et al, 2006b). Since management of pearl millet downy mildew largely depends upon host plant resistance, evolution of new virulence (s) in the pathogen population and resistance effective against new pathotypes need to be periodically monitored.…”

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confidence: 99%

Genetic Variability for Downy Mildew Disease Incidence against Virulent Downy Mildew Isolates in Mapping Population of Pearl Millet

Saritha¹,

Srivastava²,

Durgaraju³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Characterization of Sclerospora graminicola Isolates from Pearl Millet for Virulence and Genetic Diversity

Cited by 9 publications

References 11 publications

Characterisation of pathogenic and molecular diversity inSclerospora graminicola, the causal agent of pearl millet downy mildew

Characterisation of pathogenic and molecular diversity inSclerospora graminicola, the causal agent of pearl millet downy mildew

New Sources of Resistance to Multiple Pathotypes of Sclerospora graminicola in the Pearl Millet Mini Core Germplasm Collection

Genetic Variability for Downy Mildew Disease Incidence against Virulent Downy Mildew Isolates in Mapping Population of Pearl Millet

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