Characterization of SCCmec type IV methicillin-resistant Staphylococcus aureus clones increased in Japanese hospitals

Nakaminami, Hidemasa; Takadama, Shunsuke; Ito, Ayumu; Hasegawa, Masatoshi; Jono, Chika; Noguchi, Michihiko; Shoshi, Manami; Wajima, Takeaki; Fujii, Takeshi; Maruyama, Hiroshi; Sakamoto, Haruo; Ito, Yutaka; Okamoto, Satsuki; Masaki, Yukiyoshi; Tsuchiya, Kayoko; Nishinarita, Susumu; Noguchi, Norihisa

doi:10.1099/jmm.0.000735

Cited by 25 publications

(18 citation statements)

References 34 publications

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“…4 In Japan, the main HA-MRSA strains carry SCCmec type II, whereas the main CA-MRSA strains are SCCmec type IV or V. 5,6 Nevertheless, the prevalence of HA-MRSA strains with SCCmec type IV has been increasing in Japan. 3,[7][8][9] In general, HA-MRSA strains manifest high-level resistance to multiple non-b-lactam antimicrobial agents, such as quinolones, aminoglycosides and macrolides. 10 By contrast, CA-MRSA strains are usually susceptible to non-b-lactams but produce various virulence and colonization factors, such as ET, PVL and arginine catabolic mobile element (ACME).…”

Section: Introductionmentioning

confidence: 99%

Current status of Panton–Valentine leukocidin‐positive methicillin‐resistant Staphylococcus aureus isolated from patients with skin and soft tissue infections in Japan

Nakaminami

Ozawa

Sasai

et al. 2020

The Journal of Dermatology

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The USA300 clone, which produces Panton–Valentine leukocidin (PVL), is a major pathogenic community‐acquired methicillin‐resistant Staphylococcus aureus (CA‐MRSA) clone that causes intractable skin infections. Recently, PVL‐positive CA‐MRSA, including USA300 clones, have emerged in both communities and hospitals in Japan. To prevent an outbreak of PVL‐positive MRSA, infected patients should be treated with effective antimicrobial agents at community clinics. Herein, we investigate molecular epidemiological characteristics of PVL‐positive MRSA isolated from outpatients with skin and soft tissue infections (SSTI), which are common community‐onset infectious diseases. The detection rate of MRSA was 24.9% (362 strains) out of 1455 S. aureus strains isolated between 2013 and 2017. Among the MRSA strains, 15.5% (56 strains) were PVL‐positive strains and associated with deep‐seated skin infections. Molecular epidemiological analyses of PVL‐positive MRSA showed that USA300 was the predominant clone (53.6%, 30 strains) and was identified in Kanto (18 strains), Kagawa (nine strains), Tohoku (two strains) and Hokkaido (one strain). Notably, minocycline and fusidic acid were effective against all PVL‐positive MRSA strains. Hence, our data reveals the current status of PVL‐positive MRSA isolated from patients with SSTI in Japan. Continuous surveillance of CA‐MRSA is necessary to monitor latest prevalence rates and identify effective antimicrobial agents for PVL‐positive MRSA strains.

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Section: Introductionmentioning

confidence: 99%

Current status of Panton–Valentine leukocidin‐positive methicillin‐resistant Staphylococcus aureus isolated from patients with skin and soft tissue infections in Japan

Nakaminami

Ozawa

Sasai

et al. 2020

The Journal of Dermatology

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“…10 Moreover, the proportion of SCCmec type IV increased significantly from 12.4% (68 isolates) to 45.3% (185 isolates) between 2010 and 2014 in Japan. 38 SCCmec type IV (21-24 kb), merely possessed the mecA gene in general, and was considered to be movable and involving the site-specific recombinase genes. 10 Its mobility and small size are partially responsible for its common occurrence.…”

Section: Discussionmentioning

confidence: 99%

Characteristics of oral methicillin-resistant Staphylococcus epidermidis isolated from dental plaque

et al. 2020

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The oral microbial community is widely regarded as a latent reservoir of antibiotic resistance genes. This study assessed the molecular epidemiology, susceptibility profile, and resistance mechanisms of 35 methicillin-resistant Staphylococcus epidermidis (MRSE) strains isolated from the dental plaque of a healthy human population. Broth microdilution minimum inhibitory concentrations (MICs) revealed that all the isolates were nonsusceptible to oxacillin and penicillin G. Most of them were also resistant to trimethoprim (65.7%) and erythromycin (54.3%). The resistance to multiple antibiotics was found to be largely due to the acquisition of plasmid-borne genes. The mecA and dfrA genes were found in all the isolates, mostly dfrG (80%), aacA-aphD (20%), aadD (28.6%), aphA3 (22.9%), msrA (5.7%), and the ermC gene (14.3%). Classical mutational mechanisms found in these isolates were mainly efflux pumps such as qacA (31.4%), qacC (25.7%), tetK (17.1%), and norA (8.6%). Multilocus sequence type analysis revealed that sequence type 59 (ST59) strains comprised 71.43% of the typed isolates, and the eBURST algorithm clustered STs into the clonal complex 2-II(CC2-II). The staphyloccoccal cassette chromosome mec (SCCmec) type results showed that 25 (71.43%) were assigned to type IV. Moreover, 88.66% of the isolates were found to harbor six or more biofilm-associated genes. The aap, atlE, embp, sdrF, and IS256 genes were detected in all 35 isolates. This research demonstrates that biofilm-positive multipleantibiotic-resistant ST59-SCCmec IV S. epidermidis strains exist in the dental plaque of healthy people and may be a potential risk for the transmission of antibiotic resistance.

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“…In Japan, the main HA‐MRSA strains consist of SCC mec type II, whereas the main CA‐MRSA strains are SCC mec types IV or V . Nevertheless, SCC mec type IV strains have been increasing in prevalence in Japanese hospitals …”

Section: Introductionmentioning

confidence: 99%

“…4,5 In Japan, the main HA-MRSA strains consist of SCCmec type II, whereas the main CA-MRSA strains are SCCmec types IV or V. 6,7 Nevertheless, SCCmec type IV strains have been increasing in prevalence in Japanese hospitals. 3,8,9 In general, HA-MRSA strains manifest high-level resistance to multiple non-b-lactam antimicrobial agents such as quinolones, aminoglycosides and macrolides. 10 By contrast, CA-MRSA strains are usually susceptible to non-b-lactams but produce various virulence and colonization factors, such as ET, PVL and arginine catabolic mobile element (ACME).…”

Section: Introductionmentioning

confidence: 99%

Clonal change of methicillin‐resistant Staphylococcus aureus isolated from patients with impetigo in Kagawa, Japan

Sasai

Nakaminami

Iwasaki

et al. 2019

The Journal of Dermatology

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Recently, the USA300 clone, which is a Panton–Valentine leukocidin (PVL)‐positive clonal complex 8‐staphylococcal cassette chromosome mec type IV (CC8‐IV) community‐acquired methicillin‐resistant Staphylococcus aureus (CA‐MRSA) strain, emerged in community and hospital settings in Japan. Hence, clonal types of CA‐MRSA strains are predicted to be changing. Nonetheless, long‐term surveillance of CA‐MRSA has not been conducted in Japan. Here, we investigated the transition and current status of CA‐MRSA strains isolated from outpatients with impetigo; the samples were collected between 2007 and 2016 in Kagawa, Japan. The detection rate (22.8%, 488/2139 strains) of MRSA slightly decreased in these 10 years. Molecular epidemiological analyses showed that the prevalence of the CC89‐II clone, which is a typical CA‐MRSA genotype of causative agents of impetigo, significantly decreased from 48.0% (48/100 strains) in 2007–2009 to 21.9% (16/73 strains) in 2013–2016. By contrast, a non‐USA300 CC8‐IV clone, which is a highly pathogenic CA‐MRSA/J clone, significantly increased in prevalence from 9.0% (9/100 strains) to 32.9% (24/73 strains). The prevalence of PVL‐positive CA‐MRSA strains increased annually from 2012 (0%) to 2015 (6.7%), whereas only one of these strains turned out to be the USA300 clone. Antibiotic susceptibility data revealed that the rates of resistance to gentamicin and clindamycin among CA‐MRSA strains decreased along with the decreased prevalence of the CC89‐II clone and increased prevalence of the CA‐MRSA/J clone. Our data strongly suggest that the clonal types and antibiotic susceptibility of CA‐MRSA isolated from patients with impetigo dramatically changed during the last 10 years in Japan.

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Characterization of SCCmec type IV methicillin-resistant Staphylococcus aureus clones increased in Japanese hospitals

Cited by 25 publications

References 34 publications

Current status of Panton–Valentine leukocidin‐positive methicillin‐resistant Staphylococcus aureus isolated from patients with skin and soft tissue infections in Japan

Current status of Panton–Valentine leukocidin‐positive methicillin‐resistant Staphylococcus aureus isolated from patients with skin and soft tissue infections in Japan

Characteristics of oral methicillin-resistant Staphylococcus epidermidis isolated from dental plaque

Clonal change of methicillin‐resistant Staphylococcus aureus isolated from patients with impetigo in Kagawa, Japan

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