Characterization of SA12 as a simian virus 40-related papovavirus of chacma baboons

Valis, Janet D.; Newell, Nanette; Reissig, Magdalena; Malherbe, H.; Kaschula, V.R.; Shah, Keerti V.

doi:10.1128/iai.18.1.247-252.1977

Cited by 30 publications

(20 citation statements)

References 15 publications

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“…This is surprising since there is significant cross-relatedness of the V and T antigens among the primate papovaviruses BKV, JCV, and SV40 (1, 10,24,30). Two other recently described primate papovaviruses, SA12 and the stump-tailed macaque virus, also have cross-reacting T antigens with BKV, JCV, and SV40; however, the V antigens appear to be unrelated (25,31). Thus, although significant relatedness can be demonstrated among the five primate papovaviruses, no such relationship can be found between the two murine papovaviruses.…”

Section: Resultsmentioning

confidence: 99%

Characterization of K virus and its comparison with polyoma virus

Bond¹,

Howley²,

Takemoto³

1978

J Virol

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The antigenic relationship between the two murine papovaviruses, K virus and polyoma virus, was examined by serological techniques to determine whether they shared any antigenic components. No cross-reactivity was found associated with the viral (V) antigens by the indirect immunofluorescence, neutralization, or hemagglutination-inhibition tests. The tumor (T) antigens expressed in transformed cells or cells productively infected by either K or polyoma virus did not cross-react by indirect immunofluorescence. An antigenic relationship was detected, however, among the late proteins of K virus, polyoma virus, simian virus 40, and the human papovavirus BKV, when tested with either hyperimmune sera prepared against polyoma virus and simian virus 40 or sera prepared against disrupted virions. The nucleic acids of K and polyoma viruses were compared by agarose gel electrophoresis and restriction endonuclease analysis. No nucleotide sequence homology between the genomes of these two viruses was detectable by DNA-DNA hybridization techniques under stringent conditions. The genome of K virus was found to be slightly smaller than that of polyoma virus, and the cleavage patterns of the viral DNAs with six restriction endonucleases were different. These findings indicate that there is little relationship between these two murine papovaviruses.

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Section: Resultsmentioning

confidence: 99%

Characterization of K virus and its comparison with polyoma virus

Bond¹,

Howley²,

Takemoto³

1978

J Virol

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“…Although LPV shares the common antigenic determinant of all members of the polyoma virus genus, it is immunologically distinct from other members of the group. The T antigens of the primate viruses BKV, JCV, SV40, STMV, and SA12 are immunologically related (21,(24)(25)(26). However, the LPV T antigen appears to be different from the T antigens of the other primate viruses since anti-T sera against SV40, BKV, and JCV T antigens failed to react against BJA-B cells infected with LPV, which presumably were synthesizing T antigens (28; unpublished data).…”

Section: Discussionmentioning

confidence: 99%

Biological and biochemical studies of African green monkey lymphotropic papovavirus

Takemoto¹,

Furuno²,

Kato³

et al. 1982

J Virol

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Initially, virus growth was assayed by indirect immunofluorescence, using a broadly reacting anti-SV40 hamster serum which reacted against cells infected with all members of the Polyomavirus genus. In later experiments, specific anti-LPV serum made in hamsters was used. Cells and medium. Human BJA-B cells, a B-lymphoblastoid line (14), were obtained from C. Yee, National Cancer Institute, Bethesda, Md. The cells were grown in RPMI 1640 medium supplemented with 5% newborn calf serum and antibiotics. After infection with LPV, the cells were maintained in the same medium containing 2% newborn calf serum. FA procedure. Fluorescent antibody (FA) tests were performed as follows. Infected cells were centrifuged at 2,500 rpm for 5 min. The pelleted cells were suspended in a small volume of phosphate-buffered saline (pH 7.2) and smeared onto cover slips. The airdried cover slips were then fixed in acetone-methanol (1:1) for 3 min and dried. The indirect FA procedure with fluorescein-conjugated goat anti-hamster immunoglobulin was used. Preparation of LPV DNA. Viral DNA was extracted from infected BJA-B cells by the method of Hirt (12). Supercoiled form I DNA was separated from viral form II DNA and cellular DNA by the ethidium bromide buoyant density method (20). Cleavage of LPV DNA with restriction endonucleases.

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“…While initially isolated from a vervet monkey, only natural infection of baboons (Papio sp.) has been demonstrated (Valis et al, 1977).…”

Section: Baboon Polyomavirus 1 (Polyomavirus Papionis-l; Sa12)mentioning

confidence: 98%