Characterization of S haplotype in a new self-compatible Brassica rapa cultivar Dahuangyoucai

Abstract

Zhang X., Ma C., Yin D., Zhu W., Gao C., Zhang J., Fu T. (2013): Characterization of S haplotype in a new selfcompatible Brassica rapa cultivar Dahuangyoucai. Czech J. Genet. Plant Breed., 49: 157-163.The most important Brassica species, B. rapa, is naturally self-incompatible. Self-compatible mutants would be useful for dissecting the molecular mechanism of self-incompatibility (SI), a process that promotes outcrossing by recognizing and refusing self-pollens. The S haplotype in a new self-compatible B. rapa cultivar, Dahuangyoucai, was characterized for the first time in this study. Sequence analysis of the S-locus genes, SLG (S-locus glycoprotein), SRK (S-locus receptor kinase) and SCR (S-locus cysteine-rich protein) revealed that Dahuangyoucai contained S haplotype highly similar to S-f2, a non-functional class I S haplotype identified in another self-compatible B. rapa cultivar, Yellow Sarson. Mutations of MLPK (M-locus protein kinase) and non-transcription of the male determinant, SCR, were observed in this cultivar, which is similar to the situation reported in Yellow Sarson. With respect to the female determinant, SRK, no transcript was detected in Yellow Sarson but two fragments were detected in Dahuangyoucai. One fragment was highly similar to SRK-f2, but the other fragment was different from the signal factors previously identified in the SI reaction. The results suggest that Dahuangyoucai and Yellow Sarson have the same origin and a similar mechanism of self-compatibility, but diverge after mutations in SRK, SCR and MLPK. Further studying the self-compatibility of Dahuangyoucai might identify novel factors involved in the SI signalling cascade and provide new insights into the mechanisms of SI in Brassicaceae.

Recent Transposition of an LTR-Retrotransposon in the Gene Coding for S Receptor Kinase is Responsible for a Novel Self-Compatible Phenotype of Radish (Raphanus Sativus L.)

“…Among Brassica species, several SC mutants have been previously reported. In Brassica rapa, SC mutants have been identi ed from two cultivars, Yellow Sarson (Fujimoto et al 2006a) and Dahuangyoucai (Zhang et al 2013), containing similar mutant S haplotypes. Another SC phenotype induced by gene conversion from SLG to SRK has been reported (Fujimoto et al 2006b).…”

Sequence diversification and phylogenetic analysis of selfincompatibility specific determinant genes in Brassicaceae

“…But there are scanty reports using the protein data from both wild species and cultivated species for *Corresponding author's e-mail: zxhnnd@126.com Published by the Indian Society of Genetics & Plant Breeding, A-Block, F2, First Floor, NASC Complex, IARI P.O., Pusa Campus, New Delhi 110 012; Online management by indianjournals.com; www.isgpb.org phylogenetic analysis, in view that mature peptide region is better to discuss on the evolutionary point of SI determinant genes. In our previous study, an S haplotype was characterized from a self-compatible B. rapa cultivar Dahuangyoucai, which was proved to highly similar to S-f2 haplotype found in another selfcompatible B. rapa Yellow sarson, which gave rise to the confusion on the origin of S haplotype and genetic evolution (Zhang et al 2013).…”

Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology

“…Similarly a mutation at the M-locus GST gene confers SC in the sweet cherry cultivar 'Cristobalina' (Wunsch and Hormaza, 2004;Ono et al, 2018). In another example, SC turnip (Brassica rapa L.) cultivars 'Yellow Sarson' and 'Dahuangyoucai' carry indels affecting SRK and SP11 as well as a knockout point mutation in the non-S-locus gene MLPK (Zhang et al, 2013).…”