Characterization of Root-Endophytic Actinobacteria From Cactus (Opuntia Ficus-Indica) for Plant Growth Promoting Traits

Govindasamy, V.; George, Priya; Ramesh, S V; Sureshkumar, P.; Rane, Jagadish; Minhas, P. P. S.

doi:10.21203/rs.3.rs-482515/v1

Cited by 4 publications

(3 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As with other reports, the present study also found that high-G + C Gram-positive endophytic bacteria were dominant in the luffa with streptomyces being the most abundant group. These observations were similar to the study conducted by Govindasamy et al They isolated and identified endophyte actinobacteria from cactus ( Opuntia ficus-indic a) for the first time 38 .…”

Section: Discussionsupporting

confidence: 90%

Identification and antibacterial evaluation of endophytic actinobacteria from Luffa cylindrica

Mahdi

Bahrami

Kakaei

2022

Sci Rep

View full text Add to dashboard Cite

The emergence of antibiotic-resistant bacteria has limited treatment options and led to the untreatable infections, thereby necessitating the discovery of new antibiotics to battel against bacteria. Natural products from endophytic actinobacteria (EA) serve as a reservoir for discovery of new antibiotics. Therefore, the current study focused on the isolation and antibacterial properties of EA isolated from Luffa cylindrica. Six strains were identified using morphological characterization, SEM analyses and 16S rRNA gene sequencing from the roots and leaves of the plant. They were taxonomically classified as Streptomycetaceae family. This is the first report on EA form L. cylindrica. The strains produced a chain of oval, cubed or cylindrical shaped spores with spiny or smooth surfaces. Three strains; KUMS-B3, KUMS-B4 and KUMS-B6 were reported as endophytes for the first time. Fifty percent of isolates were isolated from leaves samples using YECD medium. Our results showed that the sampling time and seasons may affect the bacterial diversity. All six strains had antibacterial activity against at least one of the tested bacteria S. aureus, P. aeruginosa, and E. coli. Among the strains, KUMS-B6 isolate, closely related to S. praecox, exhibited the highest antibacterial activity against both gram-positive and negative bacteria. KUMS-B6, KUMS-B5 and KUMS-B4 isolates strongly inhibited the growth of P. aeruginosa. Interestingly, the strains, isolated from leaves exhibited stronger antagonist activities compared to those isolated from the roots. The study revealed that the isolated strains from Luffa produce a plethora of bioactive substances that are potential source of new drug candidates for the treatment of infections.

show abstract

Section: Discussionsupporting

confidence: 90%

Identification and antibacterial evaluation of endophytic actinobacteria from Luffa cylindrica

Mahdi

Bahrami

Kakaei

2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…has been reported to have potential PGP ability and enhances several vegetative parameters under salinity stress (Rangseekaew et al, 2021). Other actinobacteria, such as Micromonospora , Microbispora , Nocardia and Amycolatopsis , have also been reported to influence abiotic tolerance in plants (Govindasamy et al, 2021).…”

Section: Actinobacteria As Biotic and Abiotic Stress Relieversmentioning

confidence: 99%

Potentiality of actinobacteria to combat against biotic and abiotic stresses in tea [Camellia sinensis (L) O. Kuntze]

Borah

Hazarika

Thakur

2022

Journal of Applied Microbiology

View full text Add to dashboard Cite

Tea (Camellia sinensis (L) O. Kuntze) is a long-duration monoculture crop prone to several biotic (fungal diseases and insect pest) and abiotic (nutrient deficiency, drought and salinity) stress that eventually result in extensive annual crop loss. The specific climatic conditions and the perennial nature of the tea crop favour growth limiting abiotic factors, numerous plant pathogenic fungi (PPF) and insect pests. The review focuses on the susceptibility of tea crops to PPF/pests, drought, salinity and nutrient constraints and the potential role of beneficial actinobacteria in promoting tea crop health. The review also focuses on some of the major PPF associated with tea, such as Exobasidium vexans, Pestalotiopsis theae, Colletotrichum acutatum, and pests (Helopeltis theivora). The phylum actinobacteria own a remarkable place in agriculture due to the biosynthesis of bioactive metabolites that assist plant growth by direct nutrient assimilation, phytohormone production, and by indirect aid in plant defence against PPF and pests. The chemical diversity and bioactive significance of actinobacterial metabolites (antibiotics, siderophore, volatile organic compounds, phytohormones) are valuable in the agro-economy. This review explores the recent history of investigations in the role of actinobacteria and its secondary metabolites as a biocontrol agent and proposes a commercial application in tea cultivation.

show abstract

“…Cacti develop an association with niche soil microbes that could also contribute to the ability to overcome these stressful conditions ( Fonseca-García et al, 2016). Among them are actinobacteria belonging to Gram-positive bacteria (Govindasamy et al, 2022). Species such as cacti and their associated microbes are less explored, especially for PGPR activities of their bacteria.…”

Section: Introductionmentioning

confidence: 99%

Effect of endophytic bacteria from Algerian prickly pear roots on wheat under drought stress

Draou

GHARBI²,

SELAMI³

et al. 2022

JANS

View full text Add to dashboard Cite

Cactus are among the most drought tolerant plants. As Opuntia is able to grow under the stress of drought, this study aims to check if endophytic bacteria isolated from cactus roots have beneficial potential for crops such as wheat during drought. Two endophytic bacterial isolates were isolated from the roots of the cactus and screened for their plant growth promoting characteristics, such as N-free growth and auxin production. These bacteria have demonstrated their potential to promote the growth of durum wheat under in-vitro conditions and have been identified as Pseudomonas putida and P.brassicacearum, following the sequencing of the 16S rRNA gene and phylogenetic analysis, and significantly improved growth parameters such as seeding length compared to the unobstructed control. After 05 days of contact of the two bacteria, P.putida and P.brassicacearum, with sprouted wheat seeds, a root growth rate of (39.88% and 62.14%, respectively) was recorded. The same effect on the growth of wheat roots is caused by the volatile substances of these bacteria deposited separately, with a rate of (53.30% and 24.18%) respectively. Symptoms of drought stress were visibly reduced on seedlings inoculated with P.putida and P.brassicacearum bacteria, a result supported by a growth rate of root parameters in length (260.83% and 179.60%), surface (21.98% and 60.17%) and scope (59.46% and 62.67%), respectively. This work opens up many perspectives for the characterization and selection of endophyte bacteria of under-used drought-tolerant species such as cacti for the improvement of the growth of field crops. These results promote the deployment of Pseudomonas sp as an effective biofertilizer in wheat.

show abstract

Characterization of Root-Endophytic Actinobacteria From Cactus (Opuntia Ficus-Indica) for Plant Growth Promoting Traits

Cited by 4 publications

References 33 publications

Identification and antibacterial evaluation of endophytic actinobacteria from Luffa cylindrica

Identification and antibacterial evaluation of endophytic actinobacteria from Luffa cylindrica

Potentiality of actinobacteria to combat against biotic and abiotic stresses in tea [Camellia sinensis (L) O. Kuntze]

Effect of endophytic bacteria from Algerian prickly pear roots on wheat under drought stress

Contact Info

Product

Resources

About