Characterization of Riboflavin-Producing Strains of Lactobacillus plantarum as Potential Probiotic Candidate through in vitro Assessment and Principal Component Analysis

Bhushan, Bharat; Sakhare, Sumit M.; Narayan, Kapil Singh; Kumari, Mamta; Mishra, Vijendra; Dicks, Leon M. T.

doi:10.1007/s12602-020-09696-x

Cited by 33 publications

(22 citation statements)

References 55 publications

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“…Several Lactobacillus species provide positive impacts on human health; symptomatic improvements by probiotics have been reported in cases of various hard-to-heal diseases, e.g., allergy [ 1 ], diarrhea [ 2 ], Helicobacter pylori infection [ 3 ], and irritable bowel syndrome [ 4 ]. These probiotic effects are generally strain-specific and differ depending on each strain even among Lactobacillus strains of same species [ 5 , 6 , 7 , 8 , 9 ]. Among the several criteria for selecting candidate probiotic strains of Lactobacillus spp., bile salt resistance is one of the most important selective criteria, since bile salts are well known as strong surfactants and bile exposure in gastrointestinal tract is intensely toxic for probiotic Lactobacillus species to survive and retain activity in human intestine [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Bile Salt Hydrolase and Bile Salt Resistance in a Probiotic Bacterium Lactobacillus gasseri JCM1131T

2021

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Lactobacillus gasseri is one of the most likely probiotic candidates among many Lactobacillus species. Although bile salt resistance has been defined as an important criterion for selection of probiotic candidates since it allows probiotic bacteria to survive in the gut, both its capability and its related enzyme, bile salt hydrolase (BSH), in L. gasseri is still largely unknown. Here, we report that the well-known probiotic bacterium L. gasseri JCM1131T possesses BSH activity and bile salt resistance capability. Indeed, this strain apparently showed BSH activity on the plate assay and highly tolerated the primary bile salts and even taurine-conjugated secondary bile salt. We further isolated a putative BSH enzyme (LagBSH) from strain JCM1131T and characterized the enzymatic function. The purified LagBSH protein exhibited quite high deconjugation activity for taurocholic acid and taurochenodeoxycholic acid. The lagBSH gene was constitutively expressed in strain JCM1131T, suggesting that LagBSH likely contributes to bile salt resistance of the strain and may be associated with survival capability of strain JCM1131T within the human intestine by bile detoxification. Thus, this study first demonstrated the bile salt resistance and its responsible enzyme (BSH) activity in strain JCM1131T, which further supports the importance of the typical lactic acid bacterium as probiotics.

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Section: Introductionmentioning

confidence: 99%

Identification of Bile Salt Hydrolase and Bile Salt Resistance in a Probiotic Bacterium Lactobacillus gasseri JCM1131T

2021

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“…In this context, some experiments testing the antioxidant properties of LAB have already been reported and could be used as screens. To determine the antioxidant activity of probiotics, this can be performed directly in intact cells and cell extracts of bacterial strains by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability or by using in vitro cellular models [ 67 ]. For example, coincubation of Lactobacillus species in HT-29 cells in the presence of 2 mM H 2 O 2 , and subsequent measurement of thiobarbituric-acid-reactive substances, total antioxidant status (TAS), superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and lipid peroxidation products [ 68 ].…”

Section: Target Screening To Select Bacterial Strains With Probiotic ...mentioning

confidence: 99%

Strategies for the Identification and Assessment of Bacterial Strains with Specific Probiotic Traits

et al. 2022

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Early in the 1900s, it was proposed that health could be improved and senility delayed by manipulating gut microbiota with the host-friendly bacteria found in yogurt. Later, in 1990, the medical community reconsidered this idea and today probiotics represent a developed area of research with a billion-dollar global industry. As a result, in recent decades, increased attention has been paid to the isolation and characterization of novel probiotic bacteria from fermented foods and dairy products. Most of the identified probiotic strains belong to the lactic acid bacteria group and the genus Bifidobacterium. However, current molecular-based knowledge has allowed the identification and culture of obligatory anaerobic commensal bacteria from the human gut, such as Akkermansia spp. and Faecalibacterium spp., among other human symbionts. We are aware that the identification of new strains of these species does not guarantee their probiotic effects and that each effect must be proved through in vitro and in vivo preclinical studies before clinical trials (before even considering it as a probiotic strain). In most cases, the identification and characterization of new probiotic strain candidates may lack the appropriate set of in vitro experiments allowing the next assessment steps. Here, we address some innovative strategies reported in the literature as alternatives to classical characterization: (i) identification of alternatives using whole-metagenome shotgun sequencing, metabolomics, and multi-omics analysis; and (ii) probiotic characterization based on molecular effectors and/or traits to target specific diseases (i.e., inflammatory bowel diseases, colorectal cancer, allergies, among others).

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“…Similarly, Cao et al [16] studied L. plantarum ST with significant ABTS (47%) and DPPH (59%) scavenging activity. Antioxidant activity with CFS, cell free extract and intact cells of L. plantarum and L. rhamnosus was determined by DPPH method and highest antioxidant activity with CFS followed by cell free extract and intact cells was observed [43].…”

Section: Determination Of Antioxidant Activitymentioning

confidence: 99%

In-vitro investigation of cholesterol removal, ß-galactosidase synthesis, antioxidant, and antidiabetic potential of probiotic organisms

Chanpura¹,

Gupte²

2022

J App Biol biotech

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The present study is aimed to determine some important health beneficial properties of probiotic isolates such as cholesterol removal, β-galactosidase production, antioxidant, and anti-diabetic activity. Nine probiotic isolates were screened for Bile Salt Hydrolase (BSH) activity and potential BSH producers were selected for cholesterol removal study. Isolate B11 has shown significant cholesterol removal ability (88 ± 0.2%) after 24 h and this property was also analyzed with variable concentrations, time profile, with live, and dead cells as well as from egg yolk with simulation treatment. As probiotics improve lactose intolerance, all isolates were assessed for their lactose utilizing and β-galactosidase production ability where maximum enzyme activity was observed in case of isolate D25 (226 ± 0.30 Miller units). All the isolates were also assessed for their antioxidant potential using four different methods such as reducing power assay, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, 2,2-diphenyl-1-picrylhydrazyl assay, and superoxide dismutase activity. Out of nine isolates, isolate D25 and B11 have shown significant antioxidant property. Further, these isolates were also subjected to anti-diabetic study, where isolate D25 (89 ± 0.01%) and B11 (98 ± 0.30%) have shown high α-glucosidase inhibition which indicate their effective anti-diabetic activity. Hence, overall probiotic isolate D25 and B11 have shown significant health beneficial properties and they were further identified as Lactiplantibacillus plantarum and Pediococcus pentosaceus, respectively.

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Characterization of Riboflavin-Producing Strains of Lactobacillus plantarum as Potential Probiotic Candidate through in vitro Assessment and Principal Component Analysis

Cited by 33 publications

References 55 publications

Identification of Bile Salt Hydrolase and Bile Salt Resistance in a Probiotic Bacterium Lactobacillus gasseri JCM1131T

Identification of Bile Salt Hydrolase and Bile Salt Resistance in a Probiotic Bacterium Lactobacillus gasseri JCM1131T

Strategies for the Identification and Assessment of Bacterial Strains with Specific Probiotic Traits

In-vitro investigation of cholesterol removal, ß-galactosidase synthesis, antioxidant, and antidiabetic potential of probiotic organisms

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