Characterization of Rhizobium spp. bean isolates from South-West Spain

Rodríguez-Navarro, Dulce N.; Buendía, Ana-María; Camacho, M. E.; Lucas, M. Mercedes; Santamarı́a, C.

doi:10.1016/s0038-0717(00)00074-2

Cited by 70 publications

(38 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These strains are examples of populations from the United States (n ϭ 5), Mexico (n ϭ 21), Belize (n ϭ 2), Colombia (n ϭ 10), Ecuador (n ϭ 16), Peru (n ϭ 2), Brazil (n ϭ 12), Spain (n ϭ 21), Indonesia (n ϭ 4), and Burundi (n ϭ 1). Some of these strains had been characterized previously for their plasmid profile, DNA fingerprint, and symbiotic ability (8,10,12,(19)(20)(21). The results shown in Table 1 demonstrate that nodC types ␣, ␥, and ␦ were represented in the collection, but, unlike the results of isolates from NWA shown in the preceding section, only eight strains showed nodC type ␦, namely seven strains from Ecuador and one from Peru.…”

Section: Distribution Of Nodc Alleles In a Worldwide Collection Of Rmentioning

confidence: 83%

See 1 more Smart Citation

Analysis of Rhizobium etli and of its symbiosis with wild Phaseolus vulgaris supports coevolution in centers of host diversification

Aguilar

Riva

Peltzer

2004

Proc. Natl. Acad. Sci. U.S.A.

134

135

View full text Add to dashboard Cite

Common beans (Phaseolus vulgaris) comprise three major geographic genetic pools, one in Mexico, Central America, and Colombia, another in the southern Andes, and a third in Ecuador and northern Peru. Species Rhizobium etli is the predominant rhizobia found symbiotically associated with beans in the Americas. We have found polymorphism in the common nodulation gene nodC among R. etli strains from a wide range of geographical origins, which disclosed three nodC types. The different nodC alleles in American strains show varying predominance in their regional distributions in correlation with the centers of bean genetic diversification (BD centers). By cross-inoculating wild common beans from the three BD centers with soils from Mexico, Ecuador, Bolivia, and Northwestern Argentina, the R. etli populations from nodules originated from Mexican soil again showed allele predominance that was opposite to those originated from Bolivian and Argentinean soil, whereas populations from Ecuadorian soil were intermediate. These results also indicated that the preferential nodulation of beans by geographically related R. etli lineages was independent of the nodulating environment. Coinoculation of wild common beans from each of the three BD centers with an equicellular mixture of R. etli strains representative of the Mesoamerican and southern Andean lineages revealed a host-dependent distinct competitiveness: beans from the Mesoamerican genetic pool were almost exclusively nodulated by strains from their host region, whereas nodules of beans from the southern Andes were largely occupied by the geographically cognate R. etli lineages. These results suggest coevolution in the centers of host genetic diversification.Phaseolus vulgaris-rhizobia coevolution ͉ Rhizobium etli lineages ͉ symbiotic interaction ͉ nodC gene

show abstract

Section: Distribution Of Nodc Alleles In a Worldwide Collection Of Rmentioning

confidence: 83%

“…Thus, R. etli is found in regions where beans have been introduced since as early as the 16th century, after the discovery of the Americas. R. etli has been isolated from nodulated, cultivated beans in Spain, France, Austria, Kenya, Tunisia, and Indonesia (7,(10)(11)(12).…”

Section: T Is Generally Accepted That Phaseolus Vulgaris L (The Comentioning

confidence: 99%

Analysis of Rhizobium etli and of its symbiosis with wild Phaseolus vulgaris supports coevolution in centers of host diversification

Aguilar

Riva

Peltzer

2004

Proc. Natl. Acad. Sci. U.S.A.

134

135

View full text Add to dashboard Cite

show abstract

“…A P. vulgaris-nodulating biovar has been described for all of these species except R. tropici, based on the presence and conservation of reiterations of the nitrogenase reductase gene (nifH). Although R. etli and R. tropici are abundant in soils in Mesoamerica, presumably the center of origin of P. vulgaris, these two species have also been found in Europe; in particular, R. etli has been isolated from different locations in Spain (13,23) and from an Austrian soil (27).…”

mentioning

confidence: 99%

“…Rhizobium strains were grown at 30°C in PY medium (17). Most of the strains came from different regions of Spain and were classified by using multilocus enzyme electrophoresis and ribosomal DNA (rDNA) analysis (13,23). Beannodulating strains from other geographical areas were also included, as was a pea-nodulating R. leguminosarum bv.…”

mentioning

confidence: 99%

Conservation of Plasmid-Encoded Traits among Bean-Nodulating Rhizobium Species

Brom

Girard

Santos

et al. 2002

Appl Environ Microbiol

View full text Add to dashboard Cite

Rhizobium etli type strain CFN42 contains six plasmids. We analyzed the distribution of genetic markers from some of these plasmids in bean-nodulating strains belonging to different species (Rhizobium etli, Rhizobium gallicum, Rhizobium giardinii, Rhizobium leguminosarum, and Sinorhizobium fredii). Our results indicate that independent of geographic origin, R. etli strains usually share not only the pSym plasmid but also other plasmids containing symbiosis-related genes, with a similar organization. In contrast, strains belonging to other bean-nodulating species seem to have acquired only the pSym plasmid from R. etli.

show abstract

“…A diversidade e o tamanho da comunidade rizobiana nativa no solo podem variar com a presença de leguminosas hospedeiras (Parker, 1999;Andrade et al, 2002), de modo que a presença de uma determinada espécie de leguminosa no solo pode resultar no desenvolvimento de uma comunidade simbiótica específica (Rodriguez-Navarro et al, 2000;Carelli et al, 2000). Por outro lado, na ausência de uma planta hospedeira, os estresses ambientais, como os efeitos tóxicos relacionados à acidez do solo, podem diminuir significativamente a comunidade de rizóbios no solo (Kahindi et al, 1997;Santos et al, 1999;Hungria & Vargas, 2000;Andrade et al, 2002).…”

Section: Introductionunclassified

Diversidade genética, tolerância aos fatores de acidez e eficiência simbiótica de rizóbios para cornichão de solos do Rio Grande do Sul

Martins¹,

Vargas²,

Lisboa³

et al. 2011

Rev. Bras. Ciênc. Solo

View full text Add to dashboard Cite

O cornichão é uma leguminosa forrageira perene hiberno-primaveril de grande importância para o Rio Grande do Sul, destacando-se pela capacidade de se manter em solos relativamente ácidos e pouco férteis. Com este trabalho, objetivou-se a seleção de rizóbios para cornichão tolerantes à acidez e ao Al tóxico e eficientes na fixação biológica de N em solos de baixa fertilidade. Foram avaliados 52 isolados de rizóbios de Lotus spp. obtidos de solos de cinco localidades do Rio Grande do Sul. Os rizóbios foram avaliados quanto à diversidade genética, à tolerância a pH 4,2 e ao Al tóxico. Entre os rizóbios tolerantes a fatores de acidez, sete foram avaliados quanto à eficiência simbiótica com plantas, em casa de vegetação, em vasos com solo não estéril. Observou-se alta diversidade genética entre os rizóbios estudados, dos quais 16 foram tolerantes a pH 4,2 e a 50 µM de Al em meio de cultura, produzindo populações da ordem de 10(7) até 10(8) UFC mL-1. Os sete rizóbios testados em casa de vegetação superaram as estirpes atualmente recomendadas para a produção de inoculantes, o que demonstra a existência, em solos do Rio Grande do Sul, de rizóbios tolerantes à acidez do solo e eficientes como fixadores de N em plantas de cornichão.

show abstract

Characterization of Rhizobium spp. bean isolates from South-West Spain

Cited by 70 publications

References 35 publications

Analysis of Rhizobium etli and of its symbiosis with wild Phaseolus vulgaris supports coevolution in centers of host diversification

Analysis of Rhizobium etli and of its symbiosis with wild Phaseolus vulgaris supports coevolution in centers of host diversification

Conservation of Plasmid-Encoded Traits among Bean-Nodulating Rhizobium Species

Diversidade genética, tolerância aos fatores de acidez e eficiência simbiótica de rizóbios para cornichão de solos do Rio Grande do Sul

Contact Info

Product

Resources

About