Characterization of response regulator GacA involved in phaseolotoxin production, hypersensitive response and cellular processes in Pseudomonas syringae pv. actinidiae A18

Zhang, Lixin; Shi, Yanru; Wu, Zhiran; Tan, Genjia

doi:10.1016/j.pmpp.2018.07.001

Cited by 8 publications

(12 citation statements)

References 48 publications

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“…For instance, a gacA mutant of P. syringae pv. actinidiae A18 causes a reduced HR compared with the wild type (Zhang et al, 2018), but no differences were found for P. amygdali pv. tabaci 6605 (Marutani et al, 2008) and Pto DC3000 in non-host plants (Ferreiro et al, 2021).…”

Section: Regulation Of Virulence In Phytopathogenic Pseudomonasmentioning

confidence: 89%

Distinctive features of the Gac‐Rsm pathway in plant‐associated Pseudomonas

Ferreiro

Gallegos

2021

Environmental Microbiology

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This review focuses on the Gac-Rsm global regulatory pathway of plant-associated Pseudomonas from a novel perspective, analysing its differences and similarities between beneficial and phytopathogenic species and its role in plant interaction and colonization. Some technical considerations to approach the study of this system together with the questions that remain to be solved are also discussed.This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record. Please cite this article as

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Section: Regulation Of Virulence In Phytopathogenic Pseudomonasmentioning

confidence: 89%

Distinctive features of the Gac‐Rsm pathway in plant‐associated Pseudomonas

Ferreiro

Gallegos

2021

Environmental Microbiology

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“…When its motility is suppressed or reduced, the virulence of the pathogen is drastically reduced [ 38 , 73 ]. Zhang et al [ 74 ] demonstrated for Psa, that a minor ability for biofilm formation is reflected in a decrease in bacterial motility and a decrease in virulence. The Portuguese Psa collection studied here presented a similar in vitro motility performance ( Fig 4A ).…”

Section: Discussionmentioning

confidence: 99%

Distinct phenotypic behaviours within a clonal population of Pseudomonas syringae pv. actinidiae

et al. 2022

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Bacterial canker of the kiwifruit caused by the etiological agent Pseudomonas syringae pv. actinidiae is the most severe disease in kiwifruit production. Since 2008 a hypervirulent Psa biovar 3 has spread rapidly worldwide. Different genomic and phenotypic approaches have been used to understand the origin of the dissemination and geographical evolution of populations associated with this pandemic. This study aimed to characterize the genetic and phenotypic diversity of 22 Psa isolates collected in different regions of Portugal between 2013 and 2017. Genotypic and phenotypic characterization was based on Multi-Locus Sequence Analysis (MLSA), motility, IAA production, Biolog GEN III, and copper sensitivity. No polymorphisms were detected for the concatenated sequence (1950 bp) of the housekeeping genes gltA, gapA, gyrB, and rpoD. Results support the analysed Portuguese Psa isolates (2013–2017) belonging to Psa3, and MLSA indicates high genetic clonality and stability of these populations. The phenotypic analysis through Biolog revealed a heterogeneous pattern in the Psa collection and its position in the Pseudomonas complex. This heterogeneity reflects a genomic diversity that may reflect distinct adaptive trends associated with the environmental conditions and widespread. The Portuguese Psa collection showed no resistance to copper. This information is relevant to kiwi producers that predominantly use Cu-treatments to control kiwifruit bacterial canker.

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“…actinidiae strain A18. A gacA mutant of strain A18 showed complete abolition of phaseolotoxin production, and reduction of several virulence-related phenotypes, such as bacterial motility, biofilm formation, production of exopolysaccharide, and HR induction in tobacco plants ( Zhang et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

GacA reduces virulence and increases competitiveness in planta in the tumorigenic olive pathogen Pseudomonas savastanoi pv. savastanoi

Lavado-Benito,

Murillo,

Martínez-Gil

et al. 2024

Front. Plant Sci.

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GacS/GacA is a widely distributed two-component system playing an essential role as a key global regulator, although its characterization in phytopathogenic bacteria has been deeply biased, being intensively studied in pathogens of herbaceous plants but barely investigated in pathogens of woody hosts. P. savastanoi pv. savastanoi (Psv) is characterized by inducing tumours in the stem and branches of olive trees. In this work, the model strain Psv NCPPB 3335 and a mutant derivative with a complete deletion of gene gacA were subjected to RNA-Seq analyses in a minimum medium and a medium mimicking in planta conditions, accompanied by RT-qPCR analyses of selected genes and phenotypic assays. These experiments indicated that GacA participates in the regulation of at least 2152 genes in strain NCPPB 3335, representing 37.9 % of the annotated CDSs. GacA also controls the expression of diverse rsm genes, and modulates diverse phenotypes, including motility and resistance to oxidative stresses. As occurs with other P. syringae pathovars of herbaceous plants, GacA regulates the expression of the type III secretion system and cognate effectors. In addition, GacA also regulates the expression of WHOP genes, specifically encoded in P. syringe strains isolated from woody hosts, and genes for the biosynthesis of phytohormones. A gacA mutant of NCPPB 3335 showed increased virulence, producing large immature tumours with high bacterial populations, but showed a significantly reduced competitiveness in planta. Our results further extend the role of the global regulator GacA in the virulence and fitness of a P. syringae pathogen of woody hosts.

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Characterization of response regulator GacA involved in phaseolotoxin production, hypersensitive response and cellular processes in Pseudomonas syringae pv. actinidiae A18

Cited by 8 publications

References 48 publications

Distinctive features of the Gac‐Rsm pathway in plant‐associated Pseudomonas

Distinctive features of the Gac‐Rsm pathway in plant‐associated Pseudomonas

Distinct phenotypic behaviours within a clonal population of Pseudomonas syringae pv. actinidiae

GacA reduces virulence and increases competitiveness in planta in the tumorigenic olive pathogen Pseudomonas savastanoi pv. savastanoi

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