Characterization of Resistance to Squash Silverleaf Disorder in Summer Squash

Young, Kristen; Kabelka, Eileen A.

doi:10.21273/hortsci.44.5.1213

Cited by 8 publications

(6 citation statements)

References 11 publications

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“…Silvered leaves exhibit reduced photosynthetic ability, but this trait also seems to have a favorable effect on protection against aphids or plant desiccation, as silvery leaves reflect more light than non- silvery leaves [49]. A further study of the genes annotated in the corresponding QTL intervals (Additional file 6) is of interest to identify candidates involved in the variation of this trait and to manage it in breeding programs.…”

Section: Resultsmentioning

confidence: 99%

An SNP-based saturated genetic map and QTL analysis of fruit-related traits in Zucchini using Genotyping-by-sequencing

et al. 2017

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Background Cucurbita pepo is a cucurbit with growing economic importance worldwide. Zucchini morphotype is the most important within this highly variable species. Recently, transcriptome and Simple Sequence Repeat (SSR)- and Single Nucleotide Polymorphism (SNP)-based medium density maps have been reported, however further genomic tools are needed for efficient molecular breeding in the species. Our objective is to combine currently available complete transcriptomes and the Zucchini genome sequence with high throughput genotyping methods, mapping population development and extensive phenotyping to facilitate the advance of genomic research in this species.ResultsWe report the Genotyping-by-sequencing analysis of a RIL population developed from the inter subspecific cross Zucchini x Scallop (ssp. pepo x ssp. ovifera). Several thousands of SNP markers were identified and genotyped, followed by the construction of a high-density linkage map based on 7,718 SNPs (average of 386 markers/linkage group) covering 2,817.6 cM of the whole genome, which is a great improvement with respect to previous maps. A QTL analysis was performed using phenotypic data obtained from the RIL population from three environments. In total, 48 consistent QTLs for vine, flowering and fruit quality traits were detected on the basis of a multiple-environment analysis, distributed in 33 independent positions in 15 LGs, and each QTL explained 1.5–62.9% of the phenotypic variance. Eight major QTLs, which could explain greater than 20% of the phenotypic variation were detected and the underlying candidate genes identified.ConclusionsHere we report the first SNP saturated map in the species, anchored to the physical map. Additionally, several consistent QTLs related to early flowering, fruit shape and length, and rind and flesh color are reported as well as candidate genes for them. This information will enhance molecular breeding in C. pepo and will assist the gene cloning underlying the studied QTLs, helping to reveal the genetic basis of the studied processes in squash.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3439-y) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

An SNP-based saturated genetic map and QTL analysis of fruit-related traits in Zucchini using Genotyping-by-sequencing

et al. 2017

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“…)/100 mL water. This ensured the symptoms of CYSDV would not be confused with squash silverleaf disorder, a condition caused by persistent feeding by immature whiteflies [ 20 , 21 ]. Inoculated plants and controls were maintained inside the cages (BugDorm, 160 µm aperture, MegaView Science Co., Ltd. Taichung, Taiwan) in the greenhouse facility at the University of Georgia, Tifton.…”

Section: Methodsmentioning

confidence: 99%

Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash (Cucurbita pepo) Lines

Kavalappara

Bag

Luckew

et al. 2023

Viruses

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RNA silencing is a crucial mechanism of the antiviral immunity system in plants. Small RNAs guide Argonaut proteins to target viral RNA or DNA, preventing virus accumulation. Small RNA profiles in Cucurbita pepo line PI 420328 with tolerance to cucurbit yellow stunting disorder virus (CYSDV) were compared with those in Gold Star, a susceptible cultivar. The lower CYSDV symptom severity in PI 420328 correlated with lower virus titers and fewer sRNAs derived from CYSDV (vsRNA) compared to Gold Star. Elevated levels of 21- and 22-nucleotide (nt) size class vsRNAs were observed in PI 420328, indicating more robust and efficient RNA silencing in PI 420328. The distribution of vsRNA hotspots along the CYSDV genome was similar in both PI 420328 and Gold Star. However, the 3’ UTRs, CPm, and p26 were targeted at a higher frequency in PI 420328.

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“…A single F 1 plant was self-pollinated to create F 2 progeny and crossed with both parents to create reciprocal backcross (BC) progenies. The parents, F 1 , F 2 and BC 1 progenies were evaluated for response to silverleaf whitefly infestation and resistance to SSL disorder in 'Zuc76' was determined to be conferred by a single recessive gene (Young and Kabelka 2009). From this study, 93 F 2 progeny, segregating 3:1 (susceptible: resistant), and 30 BC 1 [(BKB 9 'Zuc76') 9 'Zuc76'] progeny, segregating 1:1 (susceptible:resistant), were used to identify molecular markers associated with SSL disorder.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Genomic DNAs of previously harvested newly expanded true leaves of 'Zuc76', 'Black Beauty', F 1 (BKB 9 'Zuc76'), 93 F 2 , and 30 BC 1 [(BKB 9 'Zuc76') 9 'Zuc76'] progeny (Young and Kabelka 2009) were isolated using a modified CTAB method. Lyophilized leaf tissue of each sample was ground to a fine powder by vortex using five 4 mm glass beads in 15 ml centrifuge tubes.…”

Section: Dna Isolationmentioning

confidence: 99%

“…A source of resistance to SSL disorder has been bred into a Cucurbita pepo zucchini squash breeding line 'Zuc76' which has been characterized as being conferred by a single recessive gene (Young and Kabelka 2009). Conventional breeding to incorporate SSL disorder resistance into squash cultivars requires time and labor for the generation of breeding populations and the phenotypic screening of adult plants for direct selection.…”

Section: Introductionmentioning

confidence: 99%

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Identification of molecular markers associated with resistance to squash silverleaf disorder in summer squash (Cucurbita pepo)

Kabelka

Young

2009

Euphytica

Self Cite

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Squash silverleaf (SSL), caused by the silverleaf whitefly [Bemisia argentifolii (formerly known as Bemisia tabaci Gennadius, B strain)], is an important physiological disorder that affects squash (Cucurbita spp.) by reducing yield potential. Breeding squash with resistance to SSL disorder can be facilitated by using marker-assisted selection (MAS). Resistance to SSL disorder, in Cucurbita pepo, is conferred by a single recessive gene (sl). The objective of this study was to identify molecular markers associated with resistance. A zucchini squash, SSL disorder resistant breeding line, 'Zuc76' (sl/sl) and a SSL disorder susceptible zucchini cultivar 'Black Beauty' (Sl/Sl) were screened with 1,152 randomly amplified polymorphic DNA (RAPD) primers and 432 simple sequence repeat (SSR) markers to identify polymorphisms. Using F 2 and BC 1 progeny segregating for SSL disorder resistance, three RAPD (OPC07, OPL07 and OPBC16) primers and one SSR (M121) marker were found associated with sl. Fragments amplified by RAPD primer OPC07 was linked in coupling phase to sl, whereas RAPD primer OPL07 was linked in repulsion phase. RAPD primer OPBC16 and SSR marker M121 were co-dominant. The allelic order of these loci was found to be M121-sl-OPC07-OPL07-OPBC16. The closest marker to sl is M121 with an estimated genetic distance of 3.3 cM. The markers identified in this study will be useful for breeding summer squash (C. pepo) for SSL disorder resistance derived from zucchini squash breeding line 'Zuc76'.

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Characterization of Resistance to Squash Silverleaf Disorder in Summer Squash

Cited by 8 publications

References 11 publications

An SNP-based saturated genetic map and QTL analysis of fruit-related traits in Zucchini using Genotyping-by-sequencing

An SNP-based saturated genetic map and QTL analysis of fruit-related traits in Zucchini using Genotyping-by-sequencing

Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash (Cucurbita pepo) Lines

Identification of molecular markers associated with resistance to squash silverleaf disorder in summer squash (Cucurbita pepo)

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