Characterization of red pigment concentrating hormone (RPCH) in the female mud crab (Scylla olivacea) and the effect of 5-HT on its expression

Kornthong, Napamanee; Chotwiwatthanakun, Charoonroj; Chansela, Piyachat; Tinikul, Yotsawan; Cummins, Scott F.; Hanna, Peter; Sobhon, Prasert

doi:10.1016/j.ygcen.2013.01.011

Cited by 32 publications

(26 citation statements)

References 41 publications

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“…In C. quadricarinatus , expression of RPCH in the CNS, eyestalk, ovary and gut (relatively low) shows a slightly different pattern from that observed in female mud crab S. olivacea , where RPCH was found in the eyestalk, brain and ventral nerve cord, but not ovary95, however this might reflect the stage from which the tissues were assessed. Global alignment shows high identity throughout the precursor and strict conservation of the mature peptide.…”

Section: Discussionmentioning

confidence: 61%

Transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the Australian crayfish, Cherax quadricarinatus

Nguyen

Cummins

Elizur

et al. 2016

Sci Rep

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The Australian redclaw crayfish (Cherax quadricarinatus) has recently received attention as an emerging candidate for sustainable aquaculture production in Australia and worldwide. More importantly, C. quadricarinatus serves as a good model organism for the commercially important group of decapod crustaceans as it is distributed worldwide, easy to maintain in the laboratory and its reproductive cycle has been well documented. In order to better understand the key reproduction and development regulating mechanisms in decapod crustaceans, the molecular toolkit available for model organisms such as C. quadricarinatus must be expanded. However, there has been no study undertaken to establish the C. quadricarinatus neuropeptidome. Here we report a comprehensive study of the neuropeptide genes expressed in the eyestalk in the Australian crayfish C. quadricarinatus. We characterised 53 putative neuropeptide-encoding transcripts based on key features of neuropeptides as characterised in other species. Of those, 14 neuropeptides implicated in reproduction regulation were chosen for assessment of their tissue distribution using RT-PCR. Further insights are discussed in relation to current knowledge of neuropeptides in other species and potential follow up studies. Overall, the resulting data lays the foundation for future gene-based neuroendocrinology studies in C. quadricarinatus.

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Section: Discussionmentioning

confidence: 61%

Transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the Australian crayfish, Cherax quadricarinatus

Nguyen

Cummins

Elizur

et al. 2016

Sci Rep

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“…It showed that methylation was not involved in the regulation of Sp-CFSH expression during ovarian development. In addition to epigenetic regulation, the synthesis and release of neurohormone can also be regulated by some neurotransmitters in crustaceans (52)(53)(54)(55)(56), such as serotonin (5-hydroxytryptamine, 5-HT). It has been reported that 5-HT stimulates the release of neurohormones, including the crustacean hyperglycemic hormone, red pigment-dispersing hormone, neurodepressing hormone, molt-inhibiting hormone, and red pigment-concentrating hormone in the red swamp crayfish, Procambarus clarkii (57-59) and the white Pacific shrimp, Litopenaeus vannamei (60).…”

Section: Discussionmentioning

confidence: 99%

DNA Methylation Inhibits the Expression of CFSH in Mud Crab

et al. 2020

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Crustacean female sex hormone (CFSH) is a key regulator of crustacean sex differentiation. The expression of Sp-CFSH in the mud crab Scylla paramamosain showed a tissue-specific and gender-variant pattern. To explore the role of DNA methylation in Sp-CFSH expression, the 5 ′-flanking region of Sp-CFSH was cloned, and one CpG island containing 12 CpG sites was found. Results of sodium bisulfite sequencing and methylated DNA immunoprecipitation showed that CpG island methylation was stable in the eyestalk ganglion during ovarian development of the females, which was significantly lower than that in the muscle of the females and in the eyestalk ganglion of the males. Such results suggested that the involvement of DNA methylation in regulating Sp-CFSH expression followed an eyestalk ganglion-specific and gender-variant pattern. The analysis of CpG dinucleotide site methylation and activity of the site-directed mutation (SDM) reporter vector further demonstrated that methylation inhibited Sp-CFSH expression by blocking the binding of transcription factor Sp1. The finding suggested for the first time the involvement of CpG methylation in the regulation of Sp-CFSH expression.

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“…In addition to the peptides predicted here for S. olivacea, several other studies have focused on peptide identification in member of the genus Scylla (e.g., Bao et al, 2015;Huang et al, 2014aHuang et al, , 2014bKornthong et al, 2013;Tsai et al, 2008). One investigation conducted on S. paramamosain is of particular note (Bao et al, 2015), as it employed essentially the same strategy/workflow for peptide discovery that was used here for S. olivacea; in this investigation, the structures of approximately 100 mature S. paramamosain peptides were predicted via in silico transcriptome mining (Bao et al, 2015).…”

Section: Conservation Of Peptide Isoforms In Members Of the Genus Scyllamentioning

confidence: 99%

Prediction of Scylla olivacea (Crustacea; Brachyura) peptide hormones using publicly accessible transcriptome shotgun assembly (TSA) sequences

Christie

2016

General and Comparative Endocrinology

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The aquaculture of crabs from the genus Scylla is of increasing economic importance for many Southeast Asian countries. Expansion of Scylla farming has led to increased efforts to understand the physiology and behavior of these crabs, and as such, there are growing molecular resources for them. Here, publicly accessible Scylla olivacea transcriptomic data were mined for putative peptide-encoding transcripts; the proteins deduced from the identified sequences were then used to predict the structures of mature peptide hormones. Forty-nine pre/preprohormone-encoding transcripts were identified, allowing for the prediction of 187 distinct mature peptides. The identified peptides included isoforms of adipokinetic hormone-corazonin-like peptide, allatostatin A, allatostatin B, allatostatin C, bursicon β, CCHamide, corazonin, crustacean cardioactive peptide, crustacean hyperglycemic hormone/molt-inhibiting hormone, diuretic hormone 31, eclosion hormone, FMRFamide-like peptide, HIGSLYRamide, insulin-like peptide, intocin, leucokinin, myosuppressin, neuroparsin, neuropeptide F, orcokinin, pigment dispersing hormone, pyrokinin, red pigment concentrating hormone, RYamide, short neuropeptide F, SIFamide and tachykinin-related peptide, all well-known neuropeptide families. Surprisingly, the tissue used to generate the transcriptome mined here is reported to be testis. Whether or not the testis samples had neural contamination is unknown. However, if the peptides are truly produced by this reproductive organ, it could have far reaching consequences for the study of crustacean endocrinology, particularly in the area of reproductive control. Regardless, this peptidome is the largest thus far predicted for any brachyuran (true crab) species, and will serve as a foundation for future studies of peptidergic control in members of the commercially important genus Scylla.

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Characterization of red pigment concentrating hormone (RPCH) in the female mud crab (Scylla olivacea) and the effect of 5-HT on its expression

Cited by 32 publications

References 41 publications

Transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the Australian crayfish, Cherax quadricarinatus

Transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the Australian crayfish, Cherax quadricarinatus

DNA Methylation Inhibits the Expression of CFSH in Mud Crab

Prediction of Scylla olivacea (Crustacea; Brachyura) peptide hormones using publicly accessible transcriptome shotgun assembly (TSA) sequences

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