Characterization of recombinant human cementum protein 1 (hrCEMP1): Primary role in biomineralization

Villarreal-Ramírez, Eduardo; Moreno, Abel; Mas‐Oliva, Jaime; Chávez‐Pacheco, Juan Luis; Narayanan, A. Sampath; Gil-Chavarría, Ivet; Zeichner‐David, Margarita; Arzate, Higinio

doi:10.1016/j.bbrc.2009.04.072

Cited by 43 publications

(49 citation statements)

References 30 publications

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“…CEMP1, a non-collagen protein, is highly expressed by cementoblasts and subpopulations of the PDL located in the paravascular zone in the periodontium (Alvarez-Pérez et al 2006). It promotes octacalcium phosphate crystal nucleation during the biomineralization process (Villarreal-Ramírez et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Cementogenic genes in human periodontal ligament stem cells are downregulated in response to osteogenic stimulation while upregulated by vitamin C treatment

Gauthier

Tran

et al. 2016

Cell Tissue Res

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Regeneration of periodontal tissues, particularly cementum, is key to regaining periodontal attachment and health. Human periodontal ligament stem cells (hPDLSCs) have been shown to be a good cell source to regenerate periodontal tissues. However, their subpopulations and the differentiation induction in relation to cementogenic lineages is unclear. Thus, we aim to examine the expression of cementum-associated genes in PDLSC subpopulations and determine the effect of broadly used osteogenic stimulus or vitamin C (VC) on the expression of cementogenic and osteogenic genes in PDLSCs. Our real-time quantitative polymerase chain reaction (qPCR) analysis showed that cementogenic marker cementum attachment protein (CAP) expressed only slightly higher in STRO-1+/CD146+, STRO-1−/CD146+ and STRO-1−/CD146− subpopulations than in the original cell pool, while cementum protein 1 (CEMP1) expression in these subpopulations was not different from the original pool. Notably, under the stimulation with osteogenic differentiation medium, CAP and CEMP1 were down-regulated while osteogenic markers bone sialoprotein (BSP) and osteocalcin (OCN) were upregulated. Both CAP and CEMP1 were upregulated by VC treatment. Transplantation of VC-treated PDLSCs into immunocompromised mice resulted in forming significantly more ectopic cementum- and bone-like mineral tissues in vivo. Immunohistochemical analysis of the ectopic growth showed that CAP and CEMP1 were mainly expressed in the mineral tissue and in some cells of the fibrous tissues. We conclude that osteogenic stimulation is not inductive but appears to be inhibitory of cementogenic pathways, whereas VC induces cementogenic lineage commitment by PDLSCs and may be a useful stimulus for cementogenesis in periodontal regeneration.

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Section: Introductionmentioning

confidence: 99%

Cementogenic genes in human periodontal ligament stem cells are downregulated in response to osteogenic stimulation while upregulated by vitamin C treatment

Gauthier

Tran

et al. 2016

Cell Tissue Res

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“…These molecules have been shown to play an important role during cementoblast differentiation and biomineralization of cementum extracellular matrix (14). Recently, it has been shown that CEMP1 possesses a strong affinity for hydroxyapatite and promotes octacalcium phosphate crystal nucleation (15). These molecules are also expressed by paravascular cells within the periodontal ligament that represent ancestors of cementoblasts, osteoblasts and periodontal ligament fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

Human cementoblasts express enamel-associated molecules in vitro and in vivo

Núñez

Sanz

Hoz-Rodríguez

et al. 2010

Journal of Periodontal Research

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“…Evidence indicates that cementum proteins in combination with BMP 2 are possible soluble factors involved in the chemotaxis and differentiation to cementoblastic lineage (Fig. 1) [33,34,66,[79][80][81][82][83].…”

Section: Stem Cell Niche and Stem Cell Differentiationmentioning

confidence: 99%

Periodontal Stem Cells: a Historical Background and Current Perspectives

Pitaru¹,

Narayanan

Etikala

et al. 2013

Curr Oral Health Rep

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In this review we discuss the historical perspective of stem cell populations from oral tissues in light of our current understanding of stem cell biology. Stem cells and their niches have been identified in the periodontium starting from the late 1970s. Applying new criteria for the identification and characterization reveals that oral tissues comprise a multipotent primitive neural crest-like stem cell population capable of differentiating into neural crest derived cell lineages of the cranial-facial zone. This population supplies cells to a more restricted stem cell type with tissue specific epigenetic memory that differentiates into cell lineages characteristic of their tissue origin. We believe that the microenvironment plays an essential role in maintaining stem cell populations and directing their migration and differentiation, and that this factor needs to be considered for utilization of stem cell-based therapy for periodontal regeneration and regenerative dental medicine.

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Characterization of recombinant human cementum protein 1 (hrCEMP1): Primary role in biomineralization

Cited by 43 publications

References 30 publications

Cementogenic genes in human periodontal ligament stem cells are downregulated in response to osteogenic stimulation while upregulated by vitamin C treatment

Cementogenic genes in human periodontal ligament stem cells are downregulated in response to osteogenic stimulation while upregulated by vitamin C treatment

Human cementoblasts express enamel-associated molecules in vitro and in vivo

Periodontal Stem Cells: a Historical Background and Current Perspectives

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