Characterization of recombinant glutathione reductase from Antarctic yeast Rhodotorula mucilaginosa

Wang, Xiaofei; Shi, Cuijuan; Chen, Guofu; Jiang, Jie; Zhang, Chengsheng; Qiao, Yongping; Ju, Yun; Wang, Ruiqi; Kan, Guangfeng; Wei, Haoruo; Zhu, Fengshuo

doi:10.1007/s00300-019-02603-3

Cited by 7 publications

(3 citation statements)

References 43 publications

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“…Our findings support earlier research that showed the wide prevalence of non- Saccharomyces strains from different EEs. For instance, R. mucilaginosa was isolated from EE, such as the Peninsula (Troncoso et al 2017 ), Antarctic sea (Wang et al 2019 ), oilfield (Derguine-Mecheri et al 2021 ), and Sua pan (Loeto et al 2021 ). Similarly, N. albida has been isolated from Antarctic soil samples (Białkowska et al 2017 ), snow from the south pole (Hayward et al 2021 ), and hypersaline coastal waters (Fotedar et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Unlocking the plant growth-promoting potential of yeast spp.: exploring species from the Moroccan extremophilic environment for enhanced plant growth and sustainable farming

Raklami,

Babalola,

Jemo

et al. 2024

FEMS Microbiology Letters

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In this study, we successfully isolated two distinct yeasts from Moroccan extreme environments. These yeasts were subjected to molecular characterization by analyzing their ITS regions. Our research thoroughly characterizes plant growth-promoting (PGP) abilities and their drought and salt stress tolerance. In a greenhouse assay, we examined the impact of selected yeasts on Medicago sativa’s growth. Four treatments were employed: i) control without inoculation (NI), ii) inoculation with L1, iii) inoculation with L2, and iv) inoculation with the mixture L1+L2. L1 isolated from Toubkal Mountain shared 99.83% sequence similarity to Rhodotorula mucilaginosa. Meanwhile, L2, thriving in the arid Merzouga desert, displayed a similar identity to Naganishia albida (99.84%). Yeast strains were tolerant to NaCl (2 M) and 60% PEG (polyethylene glycol P6000) in case of drought. Both strains could solubilize P and K (in the case of L2), produce IAA (up to 135 µl mL−1), have siderophores ability, and produce ACC deaminase. Isolates L1, L2, and their consortium showed that the single or combined strains inoculation of M. sativa improved plant growth, development, and nutrient assimilation. Those findings pave the way for harnessing yeast-based solutions in agricultural practices, contributing to enhanced crop productivity and environmental sustainability.

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Section: Discussionmentioning

confidence: 99%

Unlocking the plant growth-promoting potential of yeast spp.: exploring species from the Moroccan extremophilic environment for enhanced plant growth and sustainable farming

Raklami,

Babalola,

Jemo

et al. 2024

FEMS Microbiology Letters

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“…One of the most studied species in this genre is Rhodotorula mucilaginosa ( R . mucilaginosa ) due to its capacity to grow in harsh conditions such as highly contaminated environments, conditions of high salinity [ 21 ], low pH soils [ 22 ], rivers with a high concentration of heavy metals [ 23 , 24 ] and shallow temperatures (below 5 °C) [ 25 ]. Also, R. mucilaginosa can use complex sugars as a precursor of their central metabolism, unlike other microorganisms (like bacteria) that can only use simple sugars such as glucose or fructose [ 26 – 28 ].…”

Section: Introductionmentioning

confidence: 99%

Conversion of banana peel into diverse valuable metabolites using an autochthonous Rhodotorula mucilaginosa strain

et al. 2022

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Low-cost substrates are an exciting alternative for bioprocesses; however, their complexity can affect microorganism metabolism with non-desirable outcomes. This work evaluated banana peel extract (BPE) as a growth medium compared to commercial Yeast-Malt (YM) broth in the native and non-conventional yeast Rhodotorula mucilaginosa UANL-001L. The production of carotenoids, fatty acids, and exopolysaccharides (EPS) was also analyzed. Biomass concentration (3.9 g/L) and growth rate (0.069 g/h) of Rhodotorula mucilaginosa UANL-001L were obtained at 200 g/L of BPE. Yields per gram of dry biomass for carotenoids (317 µg/g) and fatty acids (0.55 g/g) showed the best results in 150 g/L of BPE, while 298 µg/g and 0.46 mg/g, respectively, were obtained in the YM broth. The highest yield of EPS was observed in 50 g/L of BPE, a two-fold increase (160.1 mg/g) compared to the YM broth (76.3 mg/g). The fatty acid characterization showed that 100 g/L of BPE produced 400% more unsaturated compounds (e.g., oleic and ricinoleic acid) than the YM broth. Altogether, these results indicate that BPE is a suitable medium for producing high-value products with potential industrial applications.

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“…The structures of these enzymes are usually flexible [14], which have made the Antarctic sea‐ice microorganisms become a new source of cold‐adapted enzymes with multiple applications. Accordingly, a certain amount of the properties and functions of some enzymes from Antarctic microbes were studied, including glutathione reductase [15], 2‐Cys Peroxiredoxin [16]. Nevertheless, the number of studies on cold‐adapted SHMTs has been relatively small compared with their orthologous mesophilic and thermophilic enzymes.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, characterization, and homology modeling of serine hydroxymethyltransferase from psychrophilic bacterium Psychrobacter sp.

Wang

Hou

et al. 2022

J Basic Microbiol

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Serine hydroxymethyltransferase (SHMT) plays a significant role in the synthesis of L-serine, purine, and thymidylate, which could be extensively applied in the treatment of cancers and the development of antibiotics. In this study, cloned from Psychrobacter sp. ANT206, a novel cold-adapted SHMT gene (psshmt, 1257 bp) encoding a protein of 418 amino acids was expressed in Escherichia coli. The homology modeling result revealed that PsSHMT owned

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Characterization of recombinant glutathione reductase from Antarctic yeast Rhodotorula mucilaginosa

Cited by 7 publications

References 43 publications

Unlocking the plant growth-promoting potential of yeast spp.: exploring species from the Moroccan extremophilic environment for enhanced plant growth and sustainable farming

Unlocking the plant growth-promoting potential of yeast spp.: exploring species from the Moroccan extremophilic environment for enhanced plant growth and sustainable farming

Conversion of banana peel into diverse valuable metabolites using an autochthonous Rhodotorula mucilaginosa strain

Molecular cloning, characterization, and homology modeling of serine hydroxymethyltransferase from psychrophilic bacterium Psychrobacter sp.

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