Characterization of receptors for osteogenic protein-1/bone morphogenetic protein-7 (OP-1/BMP-7) in rat kidneys

Dattatreyamurty, Bosukonda; Shih, Mei‐Shu; Sampath, Kuber T.; Vukičević, Slobodan

doi:10.1111/j.1523-1755.2000.00362.x

Cited by 59 publications

(35 citation statements)

References 24 publications

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“…In mesangial cells, it reduces TGF-␤1-induced extracellular matrix effects (41). Specific membrane-bound high-affinity BMP-7 receptors have also been characterized on adult rat kidney tubular cells (42), and in vitro in PTC, BMP-7 antagonizes TNF-␣-stimulated increases in expression of proinflammatory cytokines and chemokines (43). How then can we relate the antinflammatory and antifibrotic effects of BMP-7 with increased HA cable-mediated monocyte binding?…”

Section: Discussionmentioning

confidence: 99%

BMP-7 Modulates Hyaluronan-Mediated Proximal Tubular Cell-Monocyte Interaction

Selbi¹,

Motte²,

Hascall³

et al. 2004

Journal of the American Society of Nephrology

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Abstract. Increased synthesis of hyaluronan (HA) in the renal corticointerstitium has been documented in renal injury, although the functional significance of this is unclear. The aim of the work presented in the current study was to examine the role of HA in monocyte binding by proximal tubular cells (PTC). Using the PTC line HK-2, the authors show that unstimulated cells formed pericellular HA cable-like structures that bound mononuclear leukocytes via their cell surface CD44. Stimulation with bone morphogenic protein-7 (BMP-7) led to increased formation of HA cable-like structures and also a dosedependent increase in CD44-dependent binding of radiolabeled

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Section: Discussionmentioning

confidence: 99%

BMP-7 Modulates Hyaluronan-Mediated Proximal Tubular Cell-Monocyte Interaction

Selbi¹,

Motte²,

Hascall³

et al. 2004

Journal of the American Society of Nephrology

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“…BMP7 plays major roles during embryonic development, but in adult organisms expression of BMP7 and its receptors is retained in only few tissues, most prominently in the kidney (3,4,22,29). Its functions in adult kidney are presently unknown but may include promotion of differentiated epithelial phenotype in tubular cells (7,8,31).…”

mentioning

confidence: 99%

BMP7 antagonizes TGF-β-dependent fibrogenesis in mesangial cells

Wang

Hirschberg

2003

American Journal of Physiology-Renal Physiology

176

142

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Exogenous administration of recombinant human bone morphogenetic protein (BMP)-7 was recently shown to ameliorate renal glomerular and interstitial fibrosis in rodents with experimental renal diseases. We tested the hypothesis that BMP7 functions by antagonizing profibrogenic events that are induced by transforming growth factor (TGF)-β in cultured mesangial cells. Incubation of murine mesangial cells with TGF-β (50–200 pM) increased cell-associated collagen type IV and fibronectin, soluble collagen type IV, thrombospondin, and connective tissue growth factor (CTGF). Coincubation with recombinant human BMP7 (200 pM) reduced the increase of these ECM proteins and CTGF. The changes in collagen type IV and fibronectin proteins occurred without concomitant changes in collagen type α1IV and fibronectin mRNA levels, suggesting that TGF-β and BMP7 act primarily by affecting ECM protein degradation. Indeed, TGF-β decreases the levels and activity of matrix metalloprotease (MMP)-2, the major metalloprotease that is secreted by mesangial cells. Moreover, BMP7 inhibits TGF-β-induced activation of MMP2. Because TGF-β reduces the activity of MMPs through increasing plasminogen activator inhibitor (PAI)-1, we tested whether BMP7 interferes with this TGF-β effect. BMP7 reduces, by about two-thirds, the activation of a PAI-1 promoter/luciferase reporter in cells stably transfected with this construct. The findings from these studies indicate that BMP7 reduces TGF-β-induced ECM protein accumulation in cultured mesangial cells primarily by maintaining levels and activity of MMP2 partially through prevention of TGF-β-dependent upregulation of PAI-1.

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“…Among the ever growing family of BMP growth factors, BMP-4 and BMP-7 are the ones mostly involved in kidney development. BMP-7 seems to control the formation of metanephric mesenchyme and nephrons [Dudley et al, 1995Luo et al, 1995;Karsenty et al, 1996;Jena et al, 1997], while it remains expressed in the adult kidney [Bosukonda et al, 2000]. BMP 4 is an early morphogenetic factor in the kidney acting mainly on ureteric buds to promote the development of the ureter [Miyazaki et al, 2000;Raatikainen-Ahokas et al, 2000], while high concentrations of this protein in vitro inhibit branching of the ureteric epithelium and nephrogenesis [Martinez et al, 2002].…”

Section: Discussionmentioning

confidence: 99%

“…The effects of these BMPs are mediated like all other effects of these morphogenetic agents via regulation of mesenchymal-epithelial interactions and are substantiated through BMP specific receptors, BMPR-IA and BMPR-IB for BMP-2 and -4 [Martinez et al, 2001], and specifically through BMPR-II for BMP-7 [Bosukonda et al, 2000]. Expression regulation of both these ligands and receptors follows a certain timing during development; for example, although the peak for type I receptors occurs on embryonal day 18, lowering thereafter [Ikeda et al, 1996], BMP type II receptors are continuously expressed into adult life in rat kidneys [Vukicevic et al, 1998;Bosukonda et al, 2000].…”

Section: Discussionmentioning

confidence: 99%

“…Expression regulation of both these ligands and receptors follows a certain timing during development; for example, although the peak for type I receptors occurs on embryonal day 18, lowering thereafter [Ikeda et al, 1996], BMP type II receptors are continuously expressed into adult life in rat kidneys [Vukicevic et al, 1998;Bosukonda et al, 2000].…”

Section: Discussionmentioning

confidence: 99%

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Bone morphogenetic protein expression in newborn rat kidneys after prenatal exposure to radiofrequency radiation

Pyrpasopoulou

Kotoula

Cheva

et al. 2004

Bioelectromagnetics

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Effects of nonthermal radiofrequency radiation (RFR) of the global system of mobile communication (GSM) cellular phones have been as yet mostly studied at the molecular level in the context of cellular stress and proliferation, as well as neurotransmitter production and localization. In this study, a simulation model was designed for the exposure of pregnant rats to pulsed GSM-like RFR (9.4 GHz), based on the different resonant frequencies of man and rat. The power density applied was 5 mW/cm 2 , in order to avoid thermal electromagnetic effects as much as possible. Pregnant rats were exposed to RFR during days 1-3 postcoitum (p.c.) (embryogenesis, pre-implantation) and days 4-7 p.c. (early organogenesis, peri-implantation). Relative expression and localization of bone morphogenetic proteins (BMP) and their receptors (BMPR), members of a molecular family currently considered as major endocrine and autocrine morphogens and known to be involved in renal development, were investigated in newborn kidneys from RFR exposed and sham irradiated (control) rats. Semiquantitative duplex RT-PCR for BMP-4, -7, BMPR-IA, -IB, and -II showed increased BMP-4 and BMPR-IA, and decreased BMPR-II relative expression in newborn kidneys. These changes were statistically significant for BMP-4, BMPR-IA, and -II after exposure on days 1-3 p.c. (P <.001 each), and for BMP-4 and BMPR-IA after exposure on days 4-7 p.c. (P <.001 and P ¼.005, respectively). Immunohistochemistry and in situ hybridization (ISH) showed aberrant expression and localization of these molecules at the histological level. Our findings suggest that GSM-like RFR interferes with gene expression during early gestation and results in aberrations of BMP expression in the newborn. These molecular changes do not appear to affect renal organogenesis and may reflect a delay in the development of this organ. The differences of relative BMP expression after different time periods of exposure indicate the importance of timing for GSM-like RFR effects on embryonic development.

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Characterization of receptors for osteogenic protein-1/bone morphogenetic protein-7 (OP-1/BMP-7) in rat kidneys

Cited by 59 publications

References 24 publications

BMP-7 Modulates Hyaluronan-Mediated Proximal Tubular Cell-Monocyte Interaction

BMP-7 Modulates Hyaluronan-Mediated Proximal Tubular Cell-Monocyte Interaction

BMP7 antagonizes TGF-β-dependent fibrogenesis in mesangial cells

Bone morphogenetic protein expression in newborn rat kidneys after prenatal exposure to radiofrequency radiation

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