Characterization of rat and mouse acidic milk oligosaccharides based on hydrophilic interaction chromatography coupled with electrospray tandem mass spectrometry

Li, Jiaqi; Jiang, Meng; Zhou, Jiaorui; Ding, Junjie; Guo, Zhimou; Li, Ming; Ding, Fei; Chai, Wengang; Yan, Jingyu; Liang, Xinmiao

doi:10.1016/j.carbpol.2021.117734

Cited by 14 publications

(21 citation statements)

References 41 publications

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“…In the literature, m/z 421 in bovine milk was indicated as a phosphorylated dihexose and/or a sulfated dihexose [23,30]. The fragmentation process of m/z 421 most likely indicate the presence of phosphate/sulfate ester substitution on lactose which was shown in Figure 2 also as reported in literature [24,27,29]. A fragment ion at m/z 259 was detected for m/z 439 and 601, which could be evidence of phosphate/sulfate ester substitution in both regular and lactose-free milk (Figure 2).…”

Section: Structural Analysis Of Oligosaccharides By Tandem Mssupporting

confidence: 62%

“…Mass spectrometry has been used extensively both independently and coupled with LC for the investigation and structural characterization of milk oligosaccharides [19][20][21][22][23][24]. Since the gas phase basicities of some glycans and chloride are close to each other, it has been reported that the chloride adduct ion signal will be higher [25,26] therefore negative mode analysis would be performed for both neutral and acidic oligosaccharides.…”

Section: Introductionmentioning

confidence: 99%

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Glycoprofiling of Oligosaccharides of Regular and Lactose-Free Milk by Mass Spectrometry

Şahar

2022

Celal Bayar Üniversitesi Fen Bilimleri Dergisi

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Oligosaccharides from regular milk and lactose-free milk were analyzed by Electrospray Ionization interface coupled with Ion Trap Mass Spectrometry (IT-MS). The negative mode mass spectrometry of sugar compositions was obtained either by direct infusion and mass spectrometry with Liquid Chromatography (LC). Hexose (Hex) and the other oligomeric sugar components were observed in both regular and lactose-free milk. While lactose derivatives (deprotonated lactose dimer, chloride adducts, chloride dimer) in regular milk have seen to be dominated, monosaccharide derivatives (Hex-H2O, Hex, Hex-Cl) in the lactose-free milk were observed as abundant which was lytic product of lactose. Phosphate/sulfate ester substitution on lactose and sialyllactose in both regular and lactose-free milk samples were observed with similar intensity thus it has been understood that these important milk components are not digested during lactose removing process.

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Section: Structural Analysis Of Oligosaccharides By Tandem Mssupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Glycoprofiling of Oligosaccharides of Regular and Lactose-Free Milk by Mass Spectrometry

Şahar

2022

Celal Bayar Üniversitesi Fen Bilimleri Dergisi

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“…By contrast, bovine milk contains around 39 oligosaccharides at a concentration from 0.03 to 0.06 mg/ml, which is comparable to that in ovine milk (0.02 to 0.04 mg/ml), albeit lower than that in caprine milk (0.25 to 0.3 mg/ml) [3]. The milk of mice and rats contains a variety of oligosaccharides, where milk from rats, for example, contains more sulfated oligosaccharides [13,17]. Even though the oligosaccharide content of milk has been characterized for dozens of species [13], the role of these oligosaccharides during neonatal growth and development in non-human mammals remains poorly understood [3,18,19].…”

Section: The Importance and Variability Of Lactose In Milkmentioning

confidence: 82%

A Comparative Review of the Cell Biology, Biochemistry, and Genetics of Lactose Synthesis

Sadovnikova

García

Hovey

2021

J Mammary Gland Biol Neoplasia

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Lactose is the primary carbohydrate in the milk of most mammals and is unique in that it is only synthesized by epithelial cells in the mammary glands. Lactose is also essential for the development and nutrition of infants. Across species, the concentration of lactose in milk holds a strong positive correlation with overall milk volume. Additionally, there is a range of examples where the onset of lactose synthesis as well as the content of lactose in milk varies between species and throughout a lactation. Despite this diversity, the precursors, genes, proteins and ions that regulate lactose synthesis have not received the depth of study they likely deserve relative to the significance of this simple and abundant molecule. Through this review, our objective is to highlight the requirements for lactose synthesis at the biochemical, cellular and temporal levels through a comparative approach. This overview also serves as the prelude to a companion review describing the dietary, hormonal, molecular, and genetic factors that regulate lactose synthesis.

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“…But for a long period, detection of acid milk oligosaccharides has been a technical problem. In recent years, Yan et al ( 75 , 76 ) developted a new method of solid-phase extraction (SPE) with hydrophilic interaction chromatography (HILIC) followed by mass spectrometry (MS) identification for the analysis of sialylated milk oligosaccharides. Collision-induced dissociation tandem ESI-MS (ESI-CID-MS/MS) is then used for sequence and sialic acid α2-3/α2-6 linkage analysis.…”

Section: The Sialylated Components In Milkmentioning

confidence: 99%

“…Nano-liquid chromatography-chip-time of flight, Nano-LC-chip-TOF(74) Hydrophilic interaction chromatography, ESI-CID-MS/MS(75,76) Collision-induced dissociation tandem ESI-MS, SPE-HILIC-MS(75,76) …”

mentioning

confidence: 99%

Studies and Application of Sialylated Milk Components on Regulating Neonatal Gut Microbiota and Health

Wang

Ze²,

Rui

et al. 2021

Front. Nutr.

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Breast milk is rich in sialic acids (SA), which are commonly combined with milk oligosaccharides and glycoconjugates. As a functional nutrient component, SA-containing milk components have received increasing attention in recent years. Sialylated human milk oligosaccharides (HMOs) have been demonstrated to promote the growth and metabolism of beneficial gut microbiota in infants, bringing positive outcomes to intestinal health and immune function. They also exhibit antiviral and bacteriostatic activities in the intestinal mucosa of new-borns, thereby inhibiting the adhesion of pathogens to host cells. These properties play a pivotal role in regulating the intestinal microbial ecosystem and preventing the occurrence of neonatal inflammatory diseases. In addition, some recent studies also support the promoting effects of sialylated HMOs on neonatal bone and brain development. In addition to HMOs, sialylated glycoproteins and glycolipids are abundant in milk, and are also critical to neonatal health. This article reviews the current research progress in the regulation of sialylated milk oligosaccharides and glycoconjugates on neonatal gut microbiota and health.

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Characterization of rat and mouse acidic milk oligosaccharides based on hydrophilic interaction chromatography coupled with electrospray tandem mass spectrometry

Cited by 14 publications

References 41 publications

Glycoprofiling of Oligosaccharides of Regular and Lactose-Free Milk by Mass Spectrometry

Glycoprofiling of Oligosaccharides of Regular and Lactose-Free Milk by Mass Spectrometry

A Comparative Review of the Cell Biology, Biochemistry, and Genetics of Lactose Synthesis

Studies and Application of Sialylated Milk Components on Regulating Neonatal Gut Microbiota and Health

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