Characterization of rainbow trout milt collected with a catheter: semen parameters and cryopreservation success

Głogowski, J.; Kwaśnik, M; Piros, B.; Dąbrowski, Konrad; Goryczko, K.; Dobosz, Stefan; Kuźmiński, H.; Ciereszko, Andrzej

doi:10.1046/j.1365-2109.2000.00400.x

Cited by 88 publications

(61 citation statements)

References 41 publications

(71 reference statements)

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“…The possible precautions that could prolong the sperm viability may include: (i) the prevention of desiccation (especially important in the case of storage in capped containers) either by using a moisture-securing system (36) or by dilution with immobilizing diluents (28,40); (ii) lowering the storage temperature to below 1 o C, which prevents bacterial growth and stops metabolic processes in spermatozoa (36,40); (iii) optimizing gaseous atmosphere: an oxygen atmosphere can be sub-optimal, as demonstrated by Bencic et al (6); (iv) securing sterility by using sterile dilution media (21) or antibiotics (6,37) to prevent excessive bacterial growth, which was observed in some samples in the present study after 2 weeks of storage; (v) avoid urine contamination (19).…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, collected semen from 30 males were pooled in equal amounts to eliminate the effect of individual variability of gamete donors. Contamination with urine is considered as an important interference factor, randomly affecting quantitative characteristics of spermatozoa (19,31). This may play an important role in short-term storage of spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

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Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954)

Hatipoğlu¹,

Akçay²

2010

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The objective of this experiment was to evaluate spermatological parameters and fertilizing ability of short term stored semen in different extenders from endemic Abant trout (Salmo trutta abanticus T,1954). Semen was collected from 30 adult males by the hand stripping method without anesthesia. Having determined the main spermatological properties (volume, motility, movement duration, concentration and pH), the pooled samples were diluted at a 1:2 ratio with two extenders (0.3 M glucose or Ringer solution). The diluted semen was stored for 48 hours at 4°C. Following the cooled storage, motility of the spermatozoa was evaluated after 24 and 48 hours regarding the post-cooled period. Dry fertilization technique was used for the fertilization trials. Eggs were pooled from 30 females by the hand stripping method. Fertilization took place in dry plastic dishes and 600 eggs were used in each fertilization trial. The sperm-egg ratio was approximately 0.25x10 6 sperm/egg. After swelling, eggs were rinsed with hatchery water (7 o C) and batches were placed into vertical incubation trays. The experimental success was assessed from sperm motility and the percent of eyed-egg 25 days after fertilization. Average fresh semen motility was 81.4 %. According to the results of the experiment, the highest motility (67 % after 24 h, 53 % after 48 h) and movement duration (60 s after 24 h, 42 s after 48 h) were determined by using glucose extender after 24 and 48 hours storage. Fertility rate of fresh semen was 84.4 %. The highest eyed-egg rate (80.4 %) was obtained from semen stored with glucose based extender after 24 h storage. After 48 hour storage fertilization yield for the semen diluted with 0.3 M Glucose decreased to 61.9 % and to 43.8 % for the one diluted with Ringer solution. Our results indicate that glucose based extender is a better preservative than Ringer solution for the short term preservation of Abant trout semen.Key words: Abant trout, semen, short-term preservation, fertilityKısa süreli saklanmış Abant alabalığı spermatozoonlarının fertilizasyon yeteneği Özet: Bu araştırma ile, ülkemize ait endemik bir balık türü olan Abant alabalığının spermatolojik parametrelerinin değerlendirilmesi ve farklı sulandırıcılarda kısa süreli saklanan spermatozoonların fertilizasyon yeteneğinin ortaya konulması amaçlanmıştır. Bu araştırmada, 30 ergin erkek balıktan anestezi yapılmaksızın masaj yoluyla sperma alınmıştır. Birincil spermatolojik parametreler (miktar, motilite, haraket süresi, yoğunluk ve pH) belirlendikten sonra birleştirilen örnekler iki farklı sulandırıcı ile (0,3 M glukoz veya Ringer solusyonu) 1:2 (sperma:sulandırıcı) oranında sulandırılmıştır. Sulandırılan sperma 4°C'de 48 saat saklanmıştır. Saklama işlemi esnasında 24. ve 48. saatlerde motilite değerlendirmesi yapılmıştır. Fertilizasyon denemeleri için kuru fertilizasyon tekniği kullanılmıştır. Yumurtalar 30 ergin dişiden masaj yoluyla alınmıştır. Fertilizasyon kuru plastic kaplarda gerçekleştirilmiş ve her bir fertilizasyon denemesi için 600 yumurta ku...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954)

Hatipoğlu¹,

Akçay²

2010

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…Decreased osmotic pressure may be a sign of samples being contaminated with urine and is observed frequently in different species when semen is collected in controlled conditions (Glogowski et al, 2000). Hence, the results of this study indicate that semen samples used in previous studies may have been contaminated with urine to a lesser extent.…”

Section: Sperm Quality Indicatorsmentioning

confidence: 54%

“…Physiological changes caused by domestication cannot be ruled out either. In order to exclude any possible variables, including urine contamination, it is necessary to standardise the procedure of sperm sample taken from cyprinids, as it has been done for salmonids, in which a catheter is used (Glogowski et al, 2000).…”

Section: Sperm Quality Indicatorsmentioning

confidence: 99%

“…Short-term sperm storage at low temperature enables artificial fertilisation when the ovulation precedes spermiation; this is important for wild fish or valuable breeding lines (Glogowski et al, 2000). Fish sperm concentration is high, which restricts the possibility of long-term sperm storage in decreased metabolism condition (temperature from 0 to 4ºC).…”

Section: Introductionmentioning

confidence: 99%

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Spermatozoa motility and short-term sperm storage of colourful orfe (Leuciscus idus aberr orfus)

Sarosiek

Cejko

Targońska

et al. 2012

Italian Journal of Animal Science

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In this study the effect of six activating buffers on the movement parameters of sperm were determined and short-term storage of semen in TLP buffer was attempted (0.292 g NaCl; 0.012g KCl; 0.011g CaCl2; 0.004g MgCl2; 0.105g NaHCO3; 0.002g NaH2PO4; 50 mL; pH 8.6). Sperm was collected from five orfe individual, and spermiation was stimulated by means of an intraperitoneal Ovopel injection. The basic parameters of spermatozoa motility were measured after the semen was diluted with six different activating solutions, previously used successfully in other fish species. The motility analysis was conducted on a Crismas apparatus. Additionally, short-term storage of semen in TLP buffer was attempted. Subsequently, motility parameters were verified after 0 (Control), 24 and 120 h of storage at 4°C. It has been found that Lahnsteiner's buffer (100 mM NaCl, 10 mM Tris, 0.5% albumin, 199 mOsmkg -1 ) was found to be the most effective in sperm activation. In this paper, the spermatozoa motility of colourful ide is indicated for the first time. Finally, there was a successful attempt at short-term sperm storage for five days. For artificial insemination, it is very important to select the most effective solution to stimulate sperm motility. Data regarding sperm manipulation of orfe are scarce, so the aim of the study was to determine the basic sperm quality parameters of the colour ide form, i.e. Leuciscus idus aberr orfus.

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Refrigeration of rainbow trout gametes and embryos

Babiak

Dąbrowski²

2003

J. Exp. Zool.

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Prolonged access to early embryos composed of undifferentiated, totipotent blastomeres is desirable in situations when multiple collections of gametes are not possible. The objective of the present study is to examine whether the refrigeration of rainbow trout Oncorhynchus mykiss gametes and early embryos would be a suitable, reliable, and efficient tool for prolonging the availability of early developmental stages up to the advanced blastula stage. The study was conducted continuously during fall, winter, and spring spawning seasons. In all, more than 500 experimental variants were performed involving individual samples from 26 females and 33 males derived from three strains. These strains represented three possible circumstances. In optimal one, gametes from good quality donors were obtained soon after ovulation. In the two non-optimal sources, either donors were of poor genetic quality or gametes were collected from a distant location and transported as unfertilized gametes. A highly significant effect of variability of individual sample quality on efficiency of gamete and embryo refrigeration was revealed. The source of gametes significantly affected viability of refrigerated oocytes and embryos, but not spermatozoa. On average, oocytes from optimal source retained full fertilization viability for seven days of chilled storage, significantly longer than from non-optimal sources. Spermatozoa, regardless of storage method, retained full fertilization ability for the first week of storage. Refrigeration of embryos at 1.4+/-0.4 degrees C significantly slowed the development. Two- week-old embryos were still in blastula stage. Average survival rate of embryos refrigerated for 10 days and then transferred to regular incubation temperatures of 9-14 degrees C was 92% in optimal and 51 and 71% in non-optimal source variants. No effect of gamete and embryo refrigeration on the occurrence of developmental abnormalities was observed. Cumulative refrigeration of oocytes and embryos resulted in an average embryo survival rate of 71% in optimal source variants after 17 days of refrigeration (7 days oocytes+10 days embryos). The study shows that both gamete and embryo refrigeration can be successfully used as an efficient tool for prolonging availability of rainbow trout embryos in early developmental stages.

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Characterization of rainbow trout milt collected with a catheter: semen parameters and cryopreservation success

Cited by 88 publications

References 41 publications

Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954)

Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954)

Spermatozoa motility and short-term sperm storage of colourful orfe (Leuciscus idus aberr orfus)

Refrigeration of rainbow trout gametes and embryos

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