Characterization of PSPA Cell Differentiation,an Established Pig Preadipocyte Cell Line as an In Vitro Model for Pig Fat Development

Abstract

We have established a clonal porcine subcutaneous preadipocyte cell line for the first time. This cell line, named PSPA, shows a fibroblast-like phenotype and keeps on growing under growth medium even after reaching confluence. Adipose conversion occurs only when confluent cultures are stimulated with insulin, dexamethasone, biotin, pantothenate, and octanoate. Following exposure to this differentiation medium, growth was arrested immediately without mitotic expansion and PSPA cells exhibited a marked increase in intracellular lipid accumulation accompanied by changes in gene expressions directly related to fat metabolism and also in extracellular environments. Since PSPA cells were established, it has become possible to end the discussion over whether there is species specificity in the adipose conversion between pig and mouse by comparing PSPA cells and 3T3-L1 cells, the most widely studied preadipocyte cell line. Consequently, we demonstrated that responsiveness to inducers and fatty acid composition was unequal, despite being treated with the same media. Thus, species-specific differences indeed exist in adipogenesis between these two, meaning that PSPA cells would be an in vitro model specialized to improve understanding of pig adipocyte growth and differentiation in controlling the carcass fat content of pigs.

Prospects and challenges for cell-cultured fat as a novel food ingredient

“…OP9 is also murine derived and useful as a stromal line capable of rapid, high-efficiency lipid accumulation (Wolins et al, 2006). There is precedent for the establishment of adipogenic cell lines from agriculturally-relevant species, as evidenced by the porcine PSPA line and the bovine BIP lines reported in the literature, but none are commercially available (Aso et al, 1995;Nakajima, 2015). The development and commercialization of similar lines from common meat-producing species would provide an attractive source for fat production for food applications.…”

An immortal porcine preadipose cell strain for efficient production of cell-cultured fat

“…8,9 . Several porcine cell lines with adipogenic potential have also been reported that can maintain proliferation over 30 passages [10][11][12][13] . However, all cell lines have relatively low adipogenic e ciency, and none of them have been proven or available for using in cell-cultured fat.…”

An immortal porcine preadipocyte cell strain for efficient production of cell-cultured fat

“…8,9 . Several porcine cell lines with adipogenic potential have also been reported that can maintain proliferation over 30 passages [10][11][12][13] . However, all cell lines have relatively low adipogenic efficiency, and none of them have been proven or available for using in cell-cultured fat.…”