Characterization of Pseudomonas stutzeri NT-I capable of removing soluble selenium from the aqueous phase under aerobic conditions

Kuroda, Masatoshi; Notaguchi, Emi; Satô, Akiko; Yoshioka, M.; Hasegawa, Akira; Kagami, Tsubasa; Narita, Takanobu; Yamashita, Mitsuo; Sei, Kazunari; Soda, Satoshi; Ike, Michihiko

doi:10.1016/j.jbiosc.2011.05.012

Cited by 77 publications

(61 citation statements)

References 25 publications

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“…Examples are P. seleniipraecipitatus [16]; P. moraviensis stanleyae [17]; P. aeruginosa (SNT-SG1) [18]; P. stutzeri [12], and P. Stutzeri NT-I [19]. The genus Pseudomonas includes a wide variety of bacterial species.…”

Section: Batch Experiments Conducted With Washed Cell Suspensionsmentioning

confidence: 99%

“…This isolate has the same taxonomical features as P. Stutzeri NT-I, but these two strains differ in a few aspects. For example, the isolate could not reduce selenate at concentrations above 48.1 mM, nor could it reduce selenate anaerobically [19]. This study focuses specifically on Pseudomonas stutzeri NT-I.…”

Section: Batch Experiments Conducted With Washed Cell Suspensionsmentioning

confidence: 99%

“…This study focuses specifically on Pseudomonas stutzeri NT-I. The bacteria was originally isolated from the drainage water of a selenium refinery plant in Hyogo, Japan by Masashi Kuroda and his team [19].…”

Section: Batch Experiments Conducted With Washed Cell Suspensionsmentioning

confidence: 99%

“…In the study done by Kuroda et al [19], it was determined that the bacteria could reduce selenate to selenite within 6 hours and the selenite can then be further reduced to elemental selenium in another 18 hours. This information was used to determine the length of the reduction experiments.…”

Section: Reduction Rate Experimentsmentioning

confidence: 99%

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Reduction of Selenium by Pseudomonas stutzeri Nt-I: Growth, Reduction and Kinetics

Ce¹,

Emn²

2017

J Bioremediat Biodegrad

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IntroductionSelenium, first discovered in 1818, is a metalloid and chalcogen. It has multiple oxidation states, beginning with the most reduced state, namely selenate (SeO 4 2-, Se(VI)), followed by selenite (SeO 3 2-, Se(IV)), elemental selenium (Se 0 , Se(0)) and finally selenide (Se 2-). Of the selenium released into the environment, 37.5-40.6% can be ascribed to anthropogenic activities. It is released from the earth's crust by the mining of coal and various metals, oil production, use of agricultural products, as well as during the melting of non-ferrous metals [1]. Landfill ash disposal that generates toxic leachate poses a risk of groundwater contamination, which leads to the polluting of nearby water bodies [2].Selenium is mostly present in water as selenate or selenite. According to different studies, acid mine drainage waters contain selenium at concentrations ranging between 2 × 10 -4 and 6.2 × 10 -3 mM of total selenium [3]. Waste water from oil refineries in the San Francisco Bay (USA) contains relatively low concentrations of selenium of about 50-300 µg. L -1 [4]. The waste water from a selenium refinery plant in Japan contained an average of 30 mg. L -1 selenium, with the majority of the selenium present as selenite [4].The upward trend in energy production from coal, along with the burning of fossil fuels contribute to this increase in selenium release. The high concentration of these selenium oxyanions poses a problem to the environment, since they are toxic and bio-accumulate readily. In one case it was found the concentration of selenium in the apex predators (birds and humans) in the area were 2 000 times higher than in the water [4]. Elemental selenium is not soluble in water and thus has a much lower bio-availability than the oxyanions. Elemental selenium's toxicity is also much lower than that of the oxyanions [1]. AbstractBioremediation of seleniferous water is gaining more momentum, especially when it comes to bacterial reduction of the selenium oxyanions. More and more bacterial strains that are able to reduce selenium are being isolated. These bacteria need to be studied further to determine whether they are suited for industrial application. In this study, the reduction of Se(VI) to Se(0) by Pseudomonas stutzeri NT-I was examined using batch experiments with the bacteria suspended in MSM. For the determination of the optimum conditions for the growth of the bacteria, the linearized rate during the exponential phase for different conditions were compared. A pH 7, temperature of 37°C, salinity of 20 g.L -1 NaCl and initial concentration of 5 mM selenate were found to be the best at promoting growth. To determine the optimum conditions for the reduction of selenium, the amount of Se (0) recovered from the plug after 16 hours of incubation was measured. A pH of 8, temperature of 37°C and salinity of 5 g.L -1 resulted in the most Se (0) recovered. The kinetics of the reduction of Se(VI) to Se (0) was found to follow the adapted Monod equation. An increase in the initial Se(VI) concentrati...

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Section: Batch Experiments Conducted With Washed Cell Suspensionsmentioning

confidence: 99%

Section: Batch Experiments Conducted With Washed Cell Suspensionsmentioning

confidence: 99%

Section: Batch Experiments Conducted With Washed Cell Suspensionsmentioning

confidence: 99%

Section: Reduction Rate Experimentsmentioning

confidence: 99%

See 2 more Smart Citations

Reduction of Selenium by Pseudomonas stutzeri Nt-I: Growth, Reduction and Kinetics

Ce¹,

Emn²

2017

J Bioremediat Biodegrad

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“…However, bioreactors inoculated with anaerobic sludge failed to remove selenium, probably because of inhibitory substrates in wastewater. As a bioaugmentation strategy, an aerobic selenate-and selenite-reducing bacterium, Pseudomonas stutzeri NT-I 8) , was augmented to the bioreactors.…”

Section: Introductionmentioning

confidence: 99%

Biotreatment of Selenium Refinery Wastewater Using Pilot-Scale Granular Sludge and Swim-Bed Bioreactors Augmented with a Selenium-Reducing Bacterium <i>Pseudomonas stutzeri</i> NT－I

Soda

Takahashi²,

Kagami

et al. 2012

Japanese J. Wat. Treat. Biol.

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A new biological process for selenium removal from metal refinery wastewater was developed using selenate-and selenite-reducing bacteria. Bacterial reduction of selenium oxyanions into elemental selenium plays a role in soluble selenium detoxification. First, two pilot-scale anaerobic bioreactors (256 l each) were constructed. One was inoculated with granular sludge of an upflow anaerobic sludge blanket reactor (Reactor A). The other was equipped with the acrylic biomass carrier and was inoculated with suspended sludge of a sewage sludge digestion reactor (Reactor B). However, those bioreactors inoculated with anaerobic sludge failed to remove selenium. As a bioaugmentation strategy, an aerobic selenium-reducing bacterium, Pseudomonas stutzeri NT-I, was inoculated to the bioreactors with slight aeration. Regarding selenite-containing wastewater, Reactor A, where granular sludge and P. stutzeri NT-I coexisted, removed 95 % of 65 mg-Se l -1 quickly within only 2 days. Reactor B equipped with the biomass carrier and inoculated with P. stutzeri NT-I needed 5 days for 98% selenite removal. For selenate-containing wastewater, Reactor A removed only 54% of 30 mg-Se l -1 in 7 days. In stark contrast, Reactor B showed selenium removal from selenate-containing wastewater of over 90% within only 3 days. The effluent of each reactor turned deep red, indicating formation of elemental selenium, which can be removed easily from the aqueous phase because of its insoluble characteristics.

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The Role of Microbes in Detoxification and Availability of Metalloids

Saghafi

Bagherifam

Hatami

et al. 2020

Metalloids in Plants

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Characterization of Pseudomonas stutzeri NT-I capable of removing soluble selenium from the aqueous phase under aerobic conditions

Cited by 77 publications

References 25 publications

Reduction of Selenium by Pseudomonas stutzeri Nt-I: Growth, Reduction and Kinetics

Reduction of Selenium by Pseudomonas stutzeri Nt-I: Growth, Reduction and Kinetics

Biotreatment of Selenium Refinery Wastewater Using Pilot-Scale Granular Sludge and Swim-Bed Bioreactors Augmented with a Selenium-Reducing Bacterium <i>Pseudomonas stutzeri</i> NT－I

The Role of Microbes in Detoxification and Availability of Metalloids

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