Characterization of Pseudomonas Sp From Rhizosphere of Tomato Plants (Lycopersicon Esculentum) and Its Efficacy on Plant Growth Promotion

Abstract

Plant growth promoting rhizobacteria (PGPR) are known to enhance growth of plants by involving various direct and indirect mechanisms. About sixteen rhizobacterial isolates were isolated from various rhizospheric soils of tomato crops in the fields of Dehradun. These bacterial isolates were phenotypically characterized and were subjected to in vitroscreeningfor their plant growth promoting traits like ammonia andsiderophore production,production of indole acetic acid (IAA), hydrogen cyanide (HCN) and phosphate solubilization.Out of sixteen bacterial isolate, three bacterial isolates proved to have potential PGPR activities. The production of indole acetic acid (IAA) by all rhizobacteria was investigated as possible mechanism for plant growth stimulation. All rhizobacterial isolates were shown to produce IAA in vitroin presences of L-tryptophan in the culture medium. The isolates were also tested for their efficacy in seed germination and seedling vigour by using tomato seeds in roll towel method.All rhizobacterial isolates also showed an increased in both the shoot and the root length of tomato seedlings as compared to the control. The seed germination ranged from 90 percent (uninoculated control) to 98.5 percent. All the three strains GKS-V, HPR-I, HPR-III significantly improved seed germination when compared to the uninoculated control (UIC). These isolate showed significantly increased in shoot and root length as well as enhanced vigour index of 124.54 and 741.45 after 6 and 16 days respectively. From biochemical tests it was concluded that all the three selected rhizobacteria belong to Pseudomonas species.

Toluene Degrading Bacteria from the Rhizosphere of Solanum melongena Contaminated with Polycyclic Aromatic Hydrocarbon

“…Quantitative estimation of Indole acetic acid (IAA) production from rhizobacteria IAA production was detected as described by Agrawal et al, 2011;Agrawal and Agrawal, (2013). Each bacterium was cultured in nutrient broth medium and incubated at 28˚C for 48 h in a shaker incubator.…”

“…The productions of indole acetic acid (IAA) by all rhizobacteria were investigated as important mechanism for plant growth stimulation. All rhizobacterial isolates produced IAA in vitro by the addition of Ltryptophan, in the culture medium (Agrawal and Agrawal, 2013). The quantitative estimation of IAA production was calculated in the presence of 100 µg/ml tryptophan.…”

“…Agarwal et al, (2015) reported that the strains isolated from the rhizosphere of tomato plant significantly improved seed germination when compared to the uninoculated control. The strains GKS-V, HPR-I and HPR-III significantly increased shoot and root length as well as enhanced vigour index of 124.54 and 741.45 after 6 and 16 days respectively.…”

Plant Growth Promoting Rhizobacteria: Mechanism and Current Prospective

“…(40%). Similarly, Production of IAA by Bacillus is a general characteristic of rhizobacterial isolates [19].…”

Isolation and Characterization of PGPR from Roots of Ficus religiosa growing on Concrete Walls and its Effect on Plant Growth in Drought Condition

“…Plant growth promoting rhizobacteria (PGPR) are heterogeneous group of naturally occurring soil bacteria that colonize plant roots and benefit plants by providing growth promotion directly or indirectly. (Agrawal and Agrawal, 2013). PGPR are known for their ability to induce plant defence/tolerance and antagonize plant pathogens.…”