Characterization of Pseudomonas aeruginosa PB112 (JN996498) isolated from infected Labeo bata (Hamilton) by 16S rRNA gene sequence analysis and fatty acid methyl ester (FAME) analysis

Somerita, P; a,; P, K B; yopadhyay,; S, N Chatterjee

doi:10.5897/ajb12.2703

Cited by 9 publications

(2 citation statements)

References 22 publications

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“…Dumping of allochtonous micro-organisms could impact the ecological equilibria of these habitats, and change their resistance toward undesirable contaminants including bacterial pathogens (Litchman, 2010; Kinnunen et al, 2016). Among microbiological contaminants coming from combined sewer overflows or runoffs, one can detect well-known pathogens of aquatic organisms such as Pseudomonas aeruginosa and the Aeromonads which, among others, can infect eels, fishes and frogs (Hubbard, 1981; Panda et al, 2013; Tamam, 2014; Thomas et al, 2014). Furthermore, E. coli can be frequently detected, including sometimes the highly virulent shiga-toxin producers.…”

Section: Discussionmentioning

confidence: 99%

Human-Driven Microbiological Contamination of Benthic and Hyporheic Sediments of an Intermittent Peri-Urban River Assessed from MST and 16S rRNA Genetic Structure Analyses

et al. 2017

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Rivers are often challenged by fecal contaminations. The barrier effect of sediments against fecal bacteria was investigated through the use of a microbial source tracking (MST) toolbox, and by Next Generation Sequencing (NGS) of V5-V6 16S rRNA gene (rrs) sequences. Non-metric multi-dimensional scaling analysis of V5-V6 16S rRNA gene sequences differentiated bacteriomes according to their compartment of origin i.e., surface water against benthic and hyporheic sediments. Classification of these reads showed the most prevalent operating taxonomic units (OTU) to be allocated to Flavobacterium and Aquabacterium. Relative numbers of Gaiella, Haliangium, and Thermoleophilum OTU matched the observed differentiation of bacteriomes according to river compartments. OTU patterns were found impacted by combined sewer overflows (CSO) through an observed increase in diversity from the sewer to the hyporheic sediments. These changes appeared driven by direct transfers of bacterial contaminants from wastewaters but also by organic inputs favoring previously undetectable bacterial groups among sediments. These NGS datasets appeared more sensitive at tracking community changes than MST markers. The human-specific MST marker HF183 was strictly detected among CSO-impacted surface waters and not river bed sediments. The ruminant-specific DNA marker was more broadly distributed but intense bovine pollution was required to detect transfers from surface water to benthic and hyporheic sediments. Some OTU showed distribution patterns in line with these MST datasets such as those allocated to the Aeromonas, Acinetobacter, and Pseudomonas. Fecal indicators (Escherichia coli and total thermotolerant coliforms) were detected all over the river course but their concentrations were not correlated with MST ones. Overall, MST and NGS datasets suggested a poor colonization of river sediments by bovine and sewer bacterial contaminants. No environmental outbreak of these bacterial contaminants was detected.

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Section: Discussionmentioning

confidence: 99%

Human-Driven Microbiological Contamination of Benthic and Hyporheic Sediments of an Intermittent Peri-Urban River Assessed from MST and 16S rRNA Genetic Structure Analyses

et al. 2017

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“…According to the nucleotide base composition report, the greater amount of GC than AT content has been found. The conserved region of the novel sequence indicated the presence of larger amounts of G bases, followed by A bases, which gives a clear idea about the invariability of the region that plays a great role in phylogeny analysis (Panda et al 2012).…”

Section: Discussionmentioning

confidence: 94%

Histopathological tissue alterations of targeted organs of ornamental goldfish, Carassius auratus in India caused by a new pathogenic bacterial strain, Bacillus licheniformis PKBMS 16 identified on the basis of morpho- biochemical and 16S rDNA characterization

Saha,

Kundu,

Saha

et al. 2023

Preprint

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A highly pathogenic ampicillin-resistant bacteria Bacillus licheniformis have been identified from liver and kidney of symptomatic Goldfish Carassius auratus L. of India which may lead to a huge loss in the production of goldfish. Molecular characterization of the bacteria confirms its morphological and biochemical evidence. Phylogenetic analysis of the 16S rRNA gene sequence indicates Bacillus licheniformis PKBMS16 (KX235179) was most similar to those of Bacillus licheniformis, strain B3 (HM030741.1, 99%) and was placed highest bootstrap value. The pathogenic effect of the bacteria, Bacillus licheniformis PKBMS16 with a dose of 1.0 x 105 CFU/ml after 10 and 20 days of post-infection has been found to induce significant changes in histological, hematological, and serum biochemical parameters in the experimental group. Histologically, the internal organs, mainly the liver and kidney are severely damaged which may be the target organ for this isolated bacteria. The results on Bacillus licheniformis causing Bacilliosis in ornamental are worthy and significant because no reports on the in vivo pathogenicity of Bacillus species are available yet. However, this is the first reported episode from India, and it is reasonable to believe that in the experimental challenge, this bacterium produced a systemic pathology in terms of histological and hemato-biochemical alteration in Carassius auratus similar to many other known fish pathogens.

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Effect of physico-chemical properties of some pomegranate varieties from Tunisia on the infestation of fruits by Ectomyelois ceratoniae Zeller (Pyralidae) and Deudorix (Virachola) livia Klug (Lycaenidae)

Hamad,

Ben Chaaban,

Jemni

et al. 2024

Arthropod-Plant Interactions

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Characterization of Pseudomonas aeruginosa PB112 (JN996498) isolated from infected Labeo bata (Hamilton) by 16S rRNA gene sequence analysis and fatty acid methyl ester (FAME) analysis

Cited by 9 publications

References 22 publications

Human-Driven Microbiological Contamination of Benthic and Hyporheic Sediments of an Intermittent Peri-Urban River Assessed from MST and 16S rRNA Genetic Structure Analyses

Human-Driven Microbiological Contamination of Benthic and Hyporheic Sediments of an Intermittent Peri-Urban River Assessed from MST and 16S rRNA Genetic Structure Analyses

Histopathological tissue alterations of targeted organs of ornamental goldfish, Carassius auratus in India caused by a new pathogenic bacterial strain, Bacillus licheniformis PKBMS 16 identified on the basis of morpho- biochemical and 16S rDNA characterization

Effect of physico-chemical properties of some pomegranate varieties from Tunisia on the infestation of fruits by Ectomyelois ceratoniae Zeller (Pyralidae) and Deudorix (Virachola) livia Klug (Lycaenidae)

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