Characterization of Prototheca zopfii Associated with Outbreak of Bovine Clinical Mastitis in Herd of Beijing, China

Gao, Jian; Zhang, Hanqi; He, Jianzhong; He, Yanghua; Li, Shumei; Hou, Rong-guang; Wu, Qiaoxing; Gao, Yang; Han, Bo

doi:10.1007/s11046-011-9510-y

Cited by 42 publications

(58 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results presented by Milanov et al (17) were comparable; all algae isolated from milk were also unsusceptible to enrofloxacin, amoxicillin, tetracycline, penicillin, lincomycin, and novobiocin. The obtained findings revealed that the highest antimicotic activity was demonstrated by nystatin, ketoconazole, and amphotericin B, which is consistent with the results of other authors confirming a high effectiveness of nystatin and amphotericin B against algae isolated from milk of mastitis-affected cows (5,7,14,17). Moreover, the results reported by Malinowski et al (10) were similar.…”

Section: Discussionsupporting

confidence: 89%

Antimicrobial susceptibility of Prototheca zopfii isolated from bovine mastitis

Wawron

Bochniarz

Piech

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

View full text Add to dashboard Cite

The aim of the study was to evaluate the susceptibility of algae of the genus Prototheca to antifungal and antibacterial antibiotics. The study involved 27 isolates of Prototheca zopfii obtained from milk of mastitis-affected cows kept in a detached cowshed in the North-Eastern part of Poland. Analysis of P. zopfii susceptibility has demonstrated low effectiveness of both antifungal and antibacterial antibiotics. All algae isolated from milk were resistant to clotrimazole, fluconazole, econazole, flucytosine, cefoperazone, cephalexin, enrofloxacin, lincomycin, and oxytetracycline (100% of resistant isolates), as well as miconazole (92.6% of resistant isolates). Nystatin, ketoconazole, and amphotericin B showed the highest activity amongst the antifungal antibiotics (88.9% and 0.0%, 51.9% and 22.2%, 0.0% and 48.1% of susceptible and intermediate susceptible isolates, respectively). In the group of antibacterial antibiotics, the high activity against P. zopfii was observed only in the case of gentamicin, kanamycin (96.3% and 92.6% of susceptible isolates, respectively), and polymyxin B (59.3% of susceptible and 33.3% of intermediate susceptible isolates).

show abstract

Section: Discussionsupporting

confidence: 89%

Antimicrobial susceptibility of Prototheca zopfii isolated from bovine mastitis

Wawron

Bochniarz

Piech

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

View full text Add to dashboard Cite

show abstract

“…Moreover, beds for cows in the detached sand-floored cowshed were in ideal conditions for the development of algae and mammary gland infections via the galactogenic route. Similar environmental conditions favouring the development of protothecal mastitis were described by other authors (8,11,12,27). However, some authors (5, 9) did not isolate Prototheca sp.…”

Section: Discussionsupporting

confidence: 82%

“…The two remaining species, i.e. P. wickerhamii and P. blaschkeae, sporadically cause mastitis in cows (12).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Outbreak of protothecal mastitis in a herd of dairy cows in Poland

Wawron

Bochniarz

Piech

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

View full text Add to dashboard Cite

The study was carried out in a herd of 280 dairy cows in the North-Eastern part of Poland in the summer of 2011. During the period of 5-6 months before the study, mastitis cases resistant to routine antibiotic therapy were observed in this herd. Bacteriological examination of 280 milk samples collected from 70 cows with clinical or subclinical forms of mastitis was performed. Diagnosis of mastitis was made on the basis of anamnesis, clinical examination of the udder, macroscopic evaluation of secretion, California mastitis test (CMT), and results of bacteriological examination of milk. Protothecal mastitis was detected in 34 cows (12.6% of all cows in the herd). Algae belonging to Prototheca zopfii were isolated from 27 milk samples in pure cultures; in the remaining seven samples, mixed infections were identified (P. zopfii and Staphylococcus sp.). The acute form accompanied by elevated body temperature (40 C), pain and hot oedema of the udder, loss of appetite, and reluctance to move were observed in two cows immediately after delivery. The similar symptoms were also noted in three cows with mixed infections. The chronic form of protothecal mastitis was characterised by pasty oedema in the udder of slight painfulness and hard tissue consistency, as well as markedly reduced milk secretion. The macro-and microscopic changes in the mammary tissue was indicative of chronic, fusing microgranulomatous interstitial mastitis protothecosa.

show abstract

“…zopfii genotype 1 has only been isolated from the environment (27,28). In contrast, P. zopfii genotype 2 causes bovine mastitis and fatal systemic infection in dogs and has recently become a major concern worldwide (29)(30)(31)(32)(33)(34). In Germany and the United States, P. zopfii accounts for 20z-70z of bovine mastitis cases, and the proportion of infected cattle in Japan has been increasing (6,35,36).…”

Section: Introductionmentioning

confidence: 99%

Detection and Identification of Genotypes of <i>Prototheca zopfii</i> in Clinical Samples by Quantitative PCR Analysis

et al. 2013

View full text Add to dashboard Cite

SUMMARY:In this study, a specific quantitative PCR system for the detection and identification of Prototheca zopfii genotypes was developed using a TaqMan } MGB probe and ResoLight dye. The P. zopfii-specific primers 18PZF1 and 18PZR1 were generated on the basis of the alignment of the small subunit ribosomal DNA domain base sequences of the genera Chlorella and Prototheca obtained from DDBJ/EMBL/GenBank, and the TaqMan } MGB probe PZP1 was designed corresponding to this amplification region. Analysis of the melting curves of the amplicons using ResoLight dye was able to differentiate between P. zopfii genotypes 1 and 2. The specificity of this detection system was examined using strains from a culture collection (28 strains) and clinical isolates (140 strains). The TaqMan } MGB probe amplicon was detected only in reference strains of P. zopfii (n ＝ 12) and clinical isolates (n ＝ 135). Ninety-two clinical specimens from cows with mastitis (36 samples) and healthy controls (56 samples) were also tested. All isolates from milk samples (n ＝ 92) and clinical isolates (n ＝ 135) were identified as P. zopfii genotype 2.

show abstract

Characterization of Prototheca zopfii Associated with Outbreak of Bovine Clinical Mastitis in Herd of Beijing, China

Cited by 42 publications

References 22 publications

Antimicrobial susceptibility of Prototheca zopfii isolated from bovine mastitis

Antimicrobial susceptibility of Prototheca zopfii isolated from bovine mastitis

Outbreak of protothecal mastitis in a herd of dairy cows in Poland

Detection and Identification of Genotypes of <i>Prototheca zopfii</i> in Clinical Samples by Quantitative PCR Analysis

Contact Info

Product

Resources

About