Characterization of proton-transporting membranes from resting pig gastric mucosa

Autoimmune Gastric Disease Chemical and Clinical Studies with Special Reference to H+, K+-ATPase

“…This protein was not visible on staining with Coomassie blue. In studies of our own and by others it had previously been demonstrated that the 92 kDa protein dominating the unreduced membrane fraction corresponded to H+,K+-ATPase, the gastric proton pump (85,114). To further prove identity between the parietal cell antigen and the H+,K+-ATPase, the membrane fraction was solubilized in n-octylglucoside and subsequently incubated with protein A-Sepharose beads, which had been preincubated with immunoglobulins from each of five individual patients and, five controls.…”

“…It has recently been found that the enzyme consists of a protein complex that in addition to the catalytic polypeptide (now termed a subunit) contains a second peptide, p subunit (see below). The catalytic a subunit is a single peptide which migrates as a 92-95 kDa protein on SDS-PAGE (41, 85,134). Omeprazole, which specifically binds to H+,K+-ATPase (41, 86), is a lipophilic weak base with a pKa of 4.0.…”

The occurrence of auto‐antibodies in patients with gastro‐duodenal lesions

“…Vesicular membranes containing H',K+-ATPase were prepared by homogenization of pig gastric mucosa in 0.25 M sucrose containing 0.5 mM EGTA (ethylene-bis (oxyethylenenitri1o)tetra-acetic acid) followed by differential-and density-gradient centrifugations as described previously [8]. The vesicles were stored frozen in 0.2 5 M sucrose at -2 0 "C until required.…”

Effects of Ranitidine on Gastric Vesicles Containing H+, K+-Adenosine Triphosphatase in Rats

“…It has been documented that the polypeptide consisting of the catalytic subunit of H+,K+-ATPase has a molecular mass of about 94,000 daltons (17,3638). Ca2+-ATPase and Na' ,K+-ATPase, which also have the same molecular mass, are contained in the cellular membrane but not in the tubulovesicle (17,39).…”

“…Ca2+-ATPase and Na' ,K+-ATPase, which also have the same molecular mass, are contained in the cellular membrane but not in the tubulovesicle (17,39). The corpus mucosal scrapings were subjected to SDS-PAGE to quantify 94,000-dalton protein content in the corpus gastric mucosa.…”