Characterization of Proteins Responsive to Gibberellin in the Leaf-Sheath of Rice (Oryza sativa L.) Seedling Using Proteome Analysis.

Expression and Function of Proteins during Development of the Basal Region in Rice Seedlings

“…Calreticulin shifted from Number 155 to spot b and Number 161, or from a high pI spot to low pI spots. In a previous study, when leaf sheaths were elongated by GA 3 treatment, calreticulin shifted from pI 4.0 (low pI) to pI 4.3 (high pI) (25). The calreticulin shifts observed during development of the basal region and GA-stimulated elongation of leaf sheath, however, were in opposite directions.…”

“…The Basal Region and Leaf Sheath Proteome-Proteins from leaf sheaths, including the basal region, of seedlings have been analyzed because the tissue was found to be important in the regulation of the height of rice plants (16,19,25). The detected protein spots in the basal region were more abundant than in leaf sheath because the leaf sheath proteins form a subset of the total basal region proteome.…”

“…Two other sets having different pI spots were enolase, pI 5.1 (Number 193) and pI 5.0 (Number 194), increased and decreased, respectively, and voltage-dependent anion channel (VDAC) protein, pI 7.5 (Number 500) and pI 7.2 (Number 502), decreased and increased, respectively. In a previous report, twin protein spots identified as calreticulin had isoelectric points (pI values of 4.0 and 4.3) and expression levels that were changed by gibberellin (GA 3 ) treatment of the leaf sheath (25). Expression of the pI 4.0 protein was down-regulated, whereas the pI 4.3 protein was up-regulated when the leaf sheath was treated with GA 3 .…”

“…2B). Using only the IEF (for pI range around pI 3.5-8.0) gel for first dimension separation, 352 protein spots were detected by 2D-PAGE and CBB staining (16), whereas using the IEF and IPG (for high pI range pI 6.0 -10.0) gels, 431 proteins were detected (19,25). The IEF and IPG gels with gradient SDS-PAGE can resolve proteins over a wide range (from around pI 3.5 to 10).…”

“…Studies of environmental stimuli have focused on searching for marker proteins in response to stimuli, such as treatment with the plant hormones jasmonic acid (23), brassinolide (24), or gibberellin (19,25); growth under salt (26); drought (27); ozone stress (28); and infection with blast fungus (29,30) or Rice yellow mottle virus (31).…”