Characterization of proteinaceous binders in wall painting samples by microwave-assisted acid hydrolysis and GC-MS determination of amino acids

Colombini, Maria Perla; Fuoco, Roger; Giacomelli, Ambrogio; Muscatello, Beatrice

doi:10.1179/sic.1998.43.1.33

Cited by 36 publications

(30 citation statements)

References 10 publications

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“…Satisfactory results have also been obtained by extracting proteinaceous matter from paint samples with 2.5 M NH 3 [54], in which calcium carbonate and gypsum have a very low solubility. Reported extraction yields are 89% for egg protein, and higher for casein and animal glue: ammonia has the important advantage that it can be easily eliminated from the sample by evaporation, without interfering with GC analysis.…”

Section: Extractionmentioning

confidence: 71%

“…Reported extraction yields are 89% for egg protein, and higher for casein and animal glue: ammonia has the important advantage that it can be easily eliminated from the sample by evaporation, without interfering with GC analysis. This procedure is not suitable for eliminating copper ions, whose presence has been reported to lower derivatisation yields with some reagents used in HPLC [35,40] and in GC [54,57].…”

Section: Extractionmentioning

confidence: 99%

“…To reduce the risks of contamination connected with the addition of HCl solution into the sample, hydrolysis in the vapour phase has often been adopted [23,25,37,39]. The performance of vapour acidic hydrolysis has been significantly improved by carrying out the hydrolysis with 6 M HCl in a microwave oven under nitrogen atmosphere, at 8 atm and 1608C for 40 min [25].…”

Section: Hydrolysismentioning

confidence: 99%

“…Due to the presence of fluorine in the molecule, these derivatives are ideal for detection by negative-ion chemical ionisation mass spectrometry or by electron capture detector (ECD), which allows amino acid amounts at a fraction of pg level to be determined. b) Silylation of both acidic and aminic functions with formation of trimethylsilyl (TMS) derivatives [33,59] or tertbutyldimethylsilyl (TBDMS) derivatives [25,26,56,60]. Sylilation has the advantage of achieving the simultaneous derivatisation of acidic, aminic, and alcoholic functions.…”

Section: Derivatisation For Gc Analysismentioning

confidence: 99%

“…The performance of vapour acidic hydrolysis has been significantly improved by carrying out the hydrolysis with 6 M HCl in a microwave oven under nitrogen atmosphere, at 8 atm and 1608C for 40 min [25]. The energy delivered by microwaves allows peptidic linkages to be hydrolysed in less than an hour with yields better or comparable to those obtained with classical HCl hydrolysis (6N, 1108C, 24 h), thus limiting amino acid losses due to oxidation and degradation which arise using longer reaction times.…”

Section: Hydrolysismentioning

confidence: 99%

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Characterisation of proteinaceous binders in artistic paintings by chromatographic techniques

Colombini

Modugno

2004

J of Separation Science

108

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This review discusses the application of chromatographic techniques (GC, HPLC and Py-GC) for the characterisation of proteinaceous materials in artistic paintings. The focus is on the various analytical steps that are needed to determine these natural materials in paint samples, from sampling and sample pre-treatment, including various methods of hydrolysis and derivatisation for GC and HPLC, to approaches for data evaluation.

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Section: Extractionmentioning

confidence: 71%

Section: Extractionmentioning

confidence: 99%

Section: Hydrolysismentioning

confidence: 99%

Section: Derivatisation For Gc Analysismentioning

confidence: 99%

Section: Hydrolysismentioning

confidence: 99%

See 3 more Smart Citations

Characterisation of proteinaceous binders in artistic paintings by chromatographic techniques

Colombini

Modugno

2004

J of Separation Science

108

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Elemental labeling for the identification of proteinaceous-binding media in art works by ICP-MS

et al. 2011

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In the history of art, artists have used many different organic compounds to dissolve pigments and apply them onto a support to obtain a paint layer. Proteins were used with success from the Middle Ages up to the Renaissance, and the traditional protein sources were animal parts (skins, tendons and bones) or milk and eggs. Moreover, some of these materials are commonly used as adhesive. In this paper, the first application of the metallomic analytical technique to the identification of proteins in artworks is reported. Samples were derivatized with DTPA/Eu and the derivatization procedure was evaluated by matrix-assisted laser desorption/ionization time-of-flight before high performance liquid chromatography inductively coupled plasma MS analysis. This study has been carried out on laboratory models prepared in-house for method development, resulting in the correct identification of the different classes of proteinaceous binders typically used. In addition, some unknown paint layer samples have been analyzed demonstrating that the method is applicable to very small sample amounts (0.6 mg), which are compatible with the amount normally available for this kind of analysis. The results obtained demonstrate the effectiveness of the method, suggesting the potential future use as novel diagnostic tool in the scientific study of artworks.

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Collagen‐based proteinaceous binder‐pigment interaction study under UV ageing conditions by MALDI‐TOF‐MS and principal component analysis

et al. 2012

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This study focuses on acquiring information on the degradation process of proteinaceous binders due to ultra violet (UV) radiation and possible interactions owing to the presence of historical mineral pigments. With this aim, three different paint model samples were prepared according to medieval recipes, using rabbit glue as proteinaceus binders. One of these model samples contained only the binder, and the other two were prepared by mixing each of the pigments (cinnabar or azurite) with the binder (glue tempera model samples). The model samples were studied by applying Principal Component Analysis (PCA) to their mass spectra obtained with Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS). The complementary use of Fourier Transform Infrared Spectroscopy to study conformational changes of secondary structure of the proteinaceous binder is also proposed. Ageing effects on the model samples after up to 3000 h of UV irradiation were periodically analyzed by the proposed approach. PCA on MS data proved capable of identifying significant changes in the model samples, and the results suggested different aging behavior based on the pigment present. This research represents the first attempt to use this approach (PCA on MALDI-TOF-MS data) in the field of Cultural Heritage and demonstrates the potential benefits in the study of proteinaceous artistic materials for purposes of conservation and restoration.

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Characterization of proteinaceous binders in wall painting samples by microwave-assisted acid hydrolysis and GC-MS determination of amino acids

Cited by 36 publications

References 10 publications

Characterisation of proteinaceous binders in artistic paintings by chromatographic techniques

Characterisation of proteinaceous binders in artistic paintings by chromatographic techniques

Elemental labeling for the identification of proteinaceous-binding media in art works by ICP-MS

Collagen‐based proteinaceous binder‐pigment interaction study under UV ageing conditions by MALDI‐TOF‐MS and principal component analysis

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