Characterization of protein glycoforms by capillary-zone electrophoresis–nanoelectrospray mass spectrometry

Bateman, Kevin P.; White, Robert L.; Yaguchi, Makoto; Thibault, Pierre

doi:10.1016/s0021-9673(97)00937-0

Cited by 58 publications

(70 citation statements)

References 34 publications

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“…In its most recent version, CE capillary is carefully sharpened, i.e., by etching the outer capillary wall with hydrofluoric acid (HF) [44,88,[104][105][106] or mixtures of sulfuric and phosphoric acid [90], by mechanical shapening with an abrasive, by flameor laser-heated pulling [88,[107][108][109][110] or a combination of several techniques [37]. Kriger et al [109] reported a further cleaning subsequent to capillary sharpening by means of a so-called "piranha solution" (mixture of H 2 O 2 and sulfuric acid).…”

Section: Sheathless Interface (Nanospray)mentioning

confidence: 99%

“…6B, C). Conductive layers are prepared either by electrolytic deposition, sputter coating, vapor deposition [105,111], or combinations of several techniques, such as sputtering and electroplating [37,106]. However, poor adhesion and a restricted lifetime of 1-8 h [44,106,109] of Au-coated tips, especially when prepared as thin films (, 100 mm) [12], make them fit for limited service [19].…”

Section: Sheathless Interface (Nanospray)mentioning

confidence: 99%

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Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix‐assisted laser desorption/ionization and electrospray‐mass spectrometry detection

2005

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High throughput, outstanding certainty in peptide/protein identification, exceptional resolution, and quantitative information are essential pillars in proteome research. Capillary electrophoresis (CE) coupled to mass spectrometry (MS) has proven to meet these requirements. Soft ionization techniques, such as matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI), have paved the way for the story of success of CE-MS in the analysis of biomolecules and both approaches are subject of discussion in this article. Meanwhile, CE-MS is far away from representing a homogeneous field. Therefore the review will cover a vast area including the coupling of different modes of CE (capillary zone electrophoresis, capillary isoelectric foscusing, capillary electrochromatography, micellar electrokinetic chromatography, nonaqueous capillary electrophoresis) to MS as well as on-line preconcentration techniques (transient capillary isotachophoresis, solid-phase extraction, membrane preconcentration) applied to compensate for restricted detection sensitivity. Special attention is given to improvements in interfacing, namely addressing nanospray and coaxial sheath liquid design. Peptide mapping, collision-induced dissociation with subsequent tandem MS, and amendments in mass accuracy of instruments improve information validity gained from MS data. With 2-D on-line coupling of liquid chromatography (LC) and CE a further topic will be discussed. A special section is dedicated to recent attempts in establishing CE-ESI-MS in proteomics, in the clinical and diagnostic field, and in the food sector.

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Section: Sheathless Interface (Nanospray)mentioning

confidence: 99%

Section: Sheathless Interface (Nanospray)mentioning

confidence: 99%

Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix‐assisted laser desorption/ionization and electrospray‐mass spectrometry detection

2005

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“…Previously, CZE has been successfully coupled to nanoelectrospray for attomole protein characterisation, 20 screening of glycoprotein digests, 22 ppt level determination of sulfonamides 23 and, just recently, analysis of a nonionic surfactant. 24 All the reported applications used disposable, conductively coated emitters which were butted to the end of the capillary.…”

mentioning

confidence: 99%

Coupling of capillary zone electrophoresis to mass spectrometry (MS and MS/MS) via a nanoelectrospray interface for the characterisation of some β-agonists

Guček

Vreeken²,

Verheij³

1999

Rapid Commun. Mass Spectrom.

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Capillary zone electrophoresis (CZE) was coupled to mass spectrometry (MS) via a nanoelectrospray interface (nESI), using conductively coated tips (8 mm orifice) butted to the end of a fused-silica capillary. The CZE/nESI-MS system was carefully optimised to achieve a stable operation of the arrangement in order to run also MS/MS experiments. Mixtures of b-agonists were successfully separated using 5 mmol/L ammonium acetate in 80% MeOH as a buffer. The limit of detection was 500 attomole of a substance injected. The system was also investigated from the point of view of quantification of the substances. Careful measures were taken to avoid any systematic errors (evaporative loss during the electrokinetic injection and reduction of migration times due to the electrospray pull during the CE run). The data show that the response of a mass spectrometric detector in the low-femtomole range not only depends on the concentration of the analyte but also on the properties of the nanoelectrospray process itself, that is why the linearity of the response can be compromised.

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“…However, glycosylation sites of glycopeptides produced by trypsinization of complex glycoprotein mixtures are particularly difficult to identify because glycopeptides are usually present in relatively low abundance (2% to 5%) in peptide mixtures compared to the nonglycosylated peptides, and because the sugar fragments in the MS spectrum often dominate the peptide fragments due to glycosidic bonds being more labile than peptide bonds. Some approaches have been developed to overcome this problem, mainly based on multistage MS (Bateman et al 1998, Demelbauer et al 2004, electron capture dissociation (ECD) (Håkansson et al 2001(Håkansson et al , 2003 or electron transfer dissociation (ETD) (Hogan et al 2005).…”

Section: Wwwintechopencommentioning

confidence: 99%

Tandem Mass Spectrometry and Glycoproteins

Dolashka¹

2012

Tandem Mass Spectrometry - Applications and Principles

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Characterization of protein glycoforms by capillary-zone electrophoresis–nanoelectrospray mass spectrometry

Cited by 58 publications

References 34 publications

Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix‐assisted laser desorption/ionization and electrospray‐mass spectrometry detection

Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix‐assisted laser desorption/ionization and electrospray‐mass spectrometry detection

Coupling of capillary zone electrophoresis to mass spectrometry (MS and MS/MS) via a nanoelectrospray interface for the characterisation of some β-agonists

Tandem Mass Spectrometry and Glycoproteins

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