Characterization of Protein Glycoforms at Intact Level by Orbitrap Mass Spectrometry

Kristensen, Dan Bach; Sloth, Trine Meiborg; Ørgaard, Martin; Jensen, Pernille Foged

doi:10.1007/978-1-0716-1241-5_2

Cited by 1 publication

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zeno-TOF 7600 is a high-resolution mass system that combines the Zeno trap pulsing with Electron Activated Dissociation (EAD) fragmentation technology to uncover previously inaccessible structural information and detect up to 20× more ions [ 43 ]. Orbitrap Exploris 480 allows high-performance, high-throughput insights, combining higher sensitivity and spectral quality for increased productivity, proteome coverage, and maximum certainty in small- and large-scale studies, as well as robust and reliable performance with integrated instrument control, data processing, and servicing software [ 44 ].…”

Section: Technological Developmentmentioning

confidence: 99%

Single-Cell Proteomics: The Critical Role of Nanotechnology

Arias-Hidalgo

Juanes-Velasco

Landeira-Viñuela

et al. 2022

IJMS

View full text Add to dashboard Cite

In single-cell analysis, biological variability can be attributed to individual cells, their specific state, and the ability to respond to external stimuli, which are determined by protein abundance and their relative alterations. Mass spectrometry (MS)-based proteomics (e.g., SCoPE-MS and SCoPE2) can be used as a non-targeted method to detect molecules across hundreds of individual cells. To achieve high-throughput investigation, novel approaches in Single-Cell Proteomics (SCP) are needed to identify and quantify proteins as accurately as possible. Controlling sample preparation prior to LC-MS analysis is critical, as it influences sensitivity, robustness, and reproducibility. Several nanotechnological approaches have been developed for the removal of cellular debris, salts, and detergents, and to facilitate systematic sample processing at the nano- and microfluidic scale. In addition, nanotechnology has enabled high-throughput proteomics analysis, which have required the improvement of software tools, such as DART-ID or DO-MS, which are also fundamental for addressing key biological questions. Single-cell proteomics has many applications in nanomedicine and biomedical research, including advanced cancer immunotherapies or biomarker characterization, among others; and novel methods allow the quantification of more than a thousand proteins while analyzing hundreds of single cells.

show abstract

Section: Technological Developmentmentioning

confidence: 99%