Characterization of protein concentrates obtained by defatting cricket, locust, and silkworm powders using one-step organic solvent extraction

Rose, Alleda; Chiu, Yu-Chun; Showman, Casey; Ku, Kang‐Mo; Jaczynski, Jacek; Matak, Kristen E.

doi:10.1016/j.lwt.2023.114876

Cited by 12 publications

(2 citation statements)

References 15 publications

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“…An escalating number of scholars have recently documented alkali extraction and acid precipitation methods for isolating proteins from natural sources, such as insects, mushrooms, and plants [ 15 ]. Multiple investigations have highlighted that these alkali extraction and acid precipitation techniques could serve as cost-effective and practical means of protein extraction in industrial settings, owing to their high recovery rates and low costs [ 16 ]. This technique is typically employed to enhance the release and dissolution of proteins during the extraction process, primarily by disrupting the dense structure of natural products to increase the protein yield [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Response Surface Methodology (RSM) for Optimizing Protein Extraction from Housefly (Musca domestica) Larvae Fed with Toad and Its Structural Characterization

Miao,

Yu,

Cheng

et al. 2024

Molecules

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With the global population on the rise, an escalating interest exists in environmentally sustainable and friendly protein sources. Insects have emerged as multifaceted resources, viewed not only as potential food items, but also as sources of traditional medicines and proteins. This study utilized response surface methodology (RSM) to ascertain the optimal extraction conditions for proteins from Musca domestica used in toad feeding, denoted as MDPs-T. The yield of MDPs-T was elevated to 18.3% ± 0.2% under these optimized conditions. Subsequently, the particle size, ζ-potentials, and structures of MDPs-T were analyzed and compared with the proteins derived from Musca domestica fed on a normal diet (MDPs-ND). This comparative analysis utilized a range of advanced techniques, involving UV spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), high-performance gel permeation chromatography (HPGPC), and scanning electron microscopy (SEM). The outcomes have revealed a marginal disparity in the physical and chemical properties between MDPs-T and MDPs-ND. Derosination led to a reduction in the particle size of the MDPs by 10.98% to 62.81%. MDPs-T exhibited a higher proportion of low-molecular-weight components relative to MDPs-ND. Additionally, in a comparative analysis of amino acids, MDPs-T displayed a greater abundance of essential and total amino acids relative to MDPs-ND. Consequently, MDPs-T holds potential as a valuable food supplement for human consumption or as a nutrient-rich feed supplement for animals.

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Section: Introductionmentioning

confidence: 99%

Response Surface Methodology (RSM) for Optimizing Protein Extraction from Housefly (Musca domestica) Larvae Fed with Toad and Its Structural Characterization

Miao,

Yu,

Cheng

et al. 2024

Molecules

View full text Add to dashboard Cite

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“…Moreover, including ammonium sulfate can promote the salting-in/out mechanisms, thus allowing higher protein content to be obtained [14]. There have been some reports on extracting the protein content of crickets using the aforementioned methods [15][16][17][18]. However, to the best of our knowledge, the effect of ammonium sulfate on the extraction yield and the physicochemical and functional properties of cricket protein isolate is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Cricket Protein Isolate Extraction: Effect of Ammonium Sulfate on Physicochemical and Functional Properties of Proteins

Edward,

Wongprasert,

Bunyakanchana

et al. 2023

Foods

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Crickets are known to be a promising alternative protein source. However, a negative consumer bias and an off-flavor have become obstacles to the use of these insects in the food industry. In this study, we extracted the protein from commercial cricket powder by employing alkaline extraction–acid precipitation and including ammonium sulfate. The physicochemical and functional properties of the proteins were determined. It was found that, upon including 60% ammonium sulfate, the cricket protein isolate (CPI) had the highest protein content (~94%, w/w). The circular dichroism results indicated that a higher amount of ammonium sulfate drastically changed the secondary structure of the CPI by decreasing its α-helix content and enhancing its surface hydrophobicity. The lowest solubility of CPI was observed at pH 5. The CPI also showed better foaming properties and oil-holding capacity (OHC) compared with the cricket powder. In conclusion, adding ammonium sulfate affected the physicochemical and functional properties of the CPI, allowing it to be used as an alternative protein in protein-enriched foods and beverages.

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Exploring the role of RNASET2 in the immune response of black soldier fly larvae

Caramella,

Baranzini,

Bruno

et al. 2024

Arch Insect Biochem Physiol

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T2 RNases are transferase‐type enzymes distributed across phyla, crucial for breaking down single‐stranded RNA molecules. In addition to their canonical function, several T2 enzymes exhibit pleiotropic roles, contributing to various biological processes, such as the immune response in invertebrates and vertebrates. This study aims at characterizing RNASET2 in the larvae of black soldier fly (BSF), Hermetia illucens, which are used for organic waste reduction and the production of valuable insect biomolecules for feed formulation and other applications. Given the exposure of BSF larvae to pathogens present in the feeding substrate, it is likely that the mechanisms of their immune response have undergone significant evolution and increased complexity. After in silico characterization of HiRNASET2, demonstrating the high conservation of this T2 homolog, we investigated the expression pattern of the enzyme in the fat body and hemocytes, two districts mainly involved in the insect immune response, in larvae challenged with bacterial infection. While no variation in HiRNASET2 expression was observed in the fat body following infection, a significant upregulation of HiRNASET2 synthesis occurred in hemocytes shortly after the injection of bacteria in the larva. The intracellular localization of HiRNASET2 in lysosomes of plasmatocytes, its extracellular association with bacteria, and the presence of a putative antimicrobial domain in the molecule, suggest its potential role in RNA clean‐up and as an alarm molecule promoting phagocytosis activation by hemocytes. These insights contribute to the characterization of the immune response of Hermetia illucens larvae and may facilitate the development of animal feedstuff enriched with highly valuable BSF bioactive compounds.

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Characterization of protein concentrates obtained by defatting cricket, locust, and silkworm powders using one-step organic solvent extraction

Cited by 12 publications

References 15 publications

Response Surface Methodology (RSM) for Optimizing Protein Extraction from Housefly (Musca domestica) Larvae Fed with Toad and Its Structural Characterization

Response Surface Methodology (RSM) for Optimizing Protein Extraction from Housefly (Musca domestica) Larvae Fed with Toad and Its Structural Characterization

Cricket Protein Isolate Extraction: Effect of Ammonium Sulfate on Physicochemical and Functional Properties of Proteins

Exploring the role of RNASET2 in the immune response of black soldier fly larvae

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