Characterization of proteases activities in Ficus carica cultivars

Abstract

In this study, we characterized protease activities of 23 Ficus carica cultivars. Extracts of fruit, branch, and leaf of Masui Dauphine, one of the most representative F. carica cultivars in Japan, exhibited gelatin‐hydrolyzing activity, both in the absence and presence of a cysteine protease‐specific inhibitor, E‐64, suggesting that not only ficin (classified as cysteine protease) but also collagenase (classified as serine protease) were involved in the digestion of gelatin. In the hydrolysis of (7‐methoxycoumarin‐4‐yl)acetyl‐l‐Lys‐l‐Pro‐l‐Leu‐Gly‐l‐Leu‐[N3‐(2,4‐dinitrophenyl)‐l‐2,3‐diaminopropionyl]‐l‐Ala‐l‐Arg‐NH2, all branch extracts of 23 F. carica cultivars exhibited the activity both in the absence and presence of cysteine protease‐specific inhibitor E‐64, indicating that they contain ficin and collagenase. During digestion of acid‐solubilized type I collagen by the branch extract of Masui Dauphine at 40–55 °C, collagen was completely digested in the absence of E‐64, while it was partially digested in the presence of the inhibitor, indicating that the manner of digestion differed between ficin and collagenase contained in the extract. These results suggest that F. carica is attractive for industrial use to digest collagen. Practical Application The industrial use of F. carica might be enhanced by efficiently utilizing these proteases and/or selecting the appropriate F. carica cultivar. Collagen is one of the targets to which our results might be applied. It is widely accepted today that collagen and its digestion products could be useful as functional food. F. carica is a potential candidate for use in not only complete but also partial digestion of collagen.

Comparison of sensitivity and rapidness of PCR, recombinase polymerase amplification, and RNA-specific amplification for detection of Rice yellow mottle virus

“…We thus used extracts of Morus australis leaf [K.K., T.T., and K.Y. manuscript in preparation] and Ficus carica leaf [11] instead. As shown in Fig.…”