Characterization of Protamine Uptake by Opossum Kidney Epithelial Cells

Nagai, Junya; Komeda, Takuji; Katagiri, Yuki; Yumoto, Ryoko; Takano, Mikihisa

doi:10.1248/bpb.b13-00553

Cited by 10 publications

(5 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Data from recent studies showed that the cationic proteins and peptides, inhibit the uptake of a nephrotoxic drug, gentamicin, which is highly accumulated in the kidneys. [ 15 16 ] Some authors report that L-arginine might have protected tubular function simply by decreasing gentamicin reabsorption in the proximal tubules. [ 17 ]…”

Section: Introductionmentioning

confidence: 99%

Protective effect of L-arginine on gentamicin-induced nephrotoxicity in rats

Başhan

Seçilmiş

et al. 2014

Indian J Pharmacol

View full text Add to dashboard Cite

Introduction:L-arginine has a protective effect on gentamicin-induced renal failure and it may decrease the tubular reabsorption of another cationic substance, gentamicin due to its cationic structure. The aim of this study is to compare the possible protective effects of L-arginine and its inactive isomer D-arginine on gentamicin-induced nephrotoxicity in rats.Materials and Methods:Wistar albino rats were housed in metabolic cages and assigned to six groups as: control group, gentamicin (100 mg/kg), gentamicin + L-arginine (2 g/l), gentamicin + D-arginine (2 g/l), gentamicin + L-arginine + Nv-nitro-L-arginine methyl ester (L-NAME) (100 mg/l) and gentamicin + D-arginine + L-NAME. Gentamicin was administered by subcutaneous injections and the other drugs were added in drinking water for seven consecutive days. The animals were killed by decapitation and intracardiac blood and urine samples were obtained on the seventh day. Blood urea nitrogen, serum creatinine, sodium, potassium, urine gamma glutamyl transferase, creatinine, sodium, potassium and gentamicin levels were measured using High Performance Liquid Chromatography (HPLC) technique.Results:Gentamicin treated group had significant increase in blood urea nitrogen, serum creatinine, fractional Na excretion and urine gamma glutamyl transferase levels, and significant decrease in creatinine clearance compared to the control group. L-arginine and D-arginine reversed these findings. L-NAME abolished the nephroprotective effect of L-arginine. The urinary levels of gentamicin were significantly increased in rats treated with L-arginine or D-arginine compared to those treated with gentamicin. L-arginine and D-arginine reversed the advanced degenerative changes due to gentamicin administration in histopathological examination.Conclusion:Our study revealed the protective effect of L-arginine on gentamicin-induced nephrotoxicity, the contribution of the cationic feature of L-arginine, and the major role of NO in this protective effect.

show abstract

Section: Introductionmentioning

confidence: 99%

Protective effect of L-arginine on gentamicin-induced nephrotoxicity in rats

Başhan

Seçilmiş

et al. 2014

Indian J Pharmacol

View full text Add to dashboard Cite

show abstract

“…Expression of PEPT2 Protein Detected by Western Blotting and Confocal Laser Scanning Microscopy The crude membrane fraction was prepared from H441 cells as described previously. 11) The expression of PEPT2 protein in the crude membrane fraction was examined by Western blotting, using rabbit polyclonal anti-PEPT2 antibodies (NBP1-59626; 1 : 1000 dilution) (Novus Biologicals, LLC, Centennial, CO, U.S.A.) as the primary antibody and peroxidase-labelled affinity purified antibodies to rabbit immunoglobulin G (IgG) (H + L) (1 : 2000 dilution) as the secondary antibody, as described previously. 5) β-Actin was detected with mouse monoclonal anti-β-actin antibodies (A2228; 1 : 2000 dilution) (Merck KGaA, Darmstadt, Germany) and used as a loading control.…”

Section: Methodsmentioning

confidence: 99%

Effect of Corticosteroids on Peptide Transporter 2 Function and Induction of Innate Immune Response by Bacterial Peptides in Alveolar Epithelial Cells

Takano

Kuriyama

Kameda

et al. 2022

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

In the lung alveolar region, the innate immune system serves as an important host defense system. We recently reported that peptide transporter 2 (PEPT2) has an essential role in the uptake of bacterial peptides and induction of innate immune response in alveolar epithelial cells. In this study, we aimed to clarify the effects of corticosteroids on PEPT2 function and PEPT2-dependent innate immune response. NCI-H441 (H441) cells were used as an in vitro model of human alveolar type II epithelial cells, and the effects of dexamethasone (DEX) and budesonide (BUD) on the transport function of PEPT2 and the innate immune response induced by bacterial peptides were examined. PEPT2 function, estimated by measuring β-alanyl-Nε-(7-amino-4-methyl-2-oxo-2H-1benzopyran-3-acetyl)-L-lysine (β-Ala-Lys-AMCA) uptake in H441 cells, was suppressed by treatment with DEX and BUD in a concentration-and time-dependent manner. The suppression of PEPT2 function was partially recovered by a glucocorticoid receptor antagonist. The expression of PEPT2 and nucleotide-binding oligomerization domain 1 (NOD1) mRNAs was suppressed by treatment with DEX and BUD, while PEPT2 protein level was not changed by these treatment conditions. Additionally, the increased mRNA expression of interleukin (IL)-8 and the increased secretion of IL-8 into the culture medium induced by bacterial peptides were also suppressed by treatment with these corticosteroids. Taken together, these results clearly suggest that corticosteroids suppress PEPT2 function and bacterial peptide-induced innate immune response in alveolar epithelial cells. Therefore, PEPT2-and NOD1-dependent innate immune response induced by bacterial peptides in the lung alveolar region may be suppressed during the inhaled corticosteroid therapy.

show abstract

“…87 PTDs cross membranes through protein transduction, facilitating the transport of fused materials across cellular membranes. 88 PTDs can be linked covalently to onaBoNT-A to facilitate their entry into any cell type independent of receptors and transporters. TAT peptide is a PTD derived from human immunodeficiency virus (HIV), which was successfully employed for the uptake of peptide nucleic acids, conjugated with TAT peptide, into rat bladders.…”

Section: Latest Developmentsmentioning

confidence: 99%