Characterization of propiconazole field-resistant isolates of Ustilaginoidea virens

Zhou, Yuxin; Yu, Junjie; X, Pan; Yu, Mina; Du, Yan; Qi, Zhongqiang; Zhang, Rongsheng; Song, Tianqiang; Yin, Xiuli; Liu, Yongfeng

doi:10.1016/j.pestbp.2018.11.013

Cited by 26 publications

(25 citation statements)

References 85 publications

(104 reference statements)

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“…In addition, overexpression of CYP51 was observed in the tebuconazole-resistant mutant (Wang et al 2015). However, in the current study, no mutations in the CYP51 gene were detected in any of the six propiconazole-resistant isolates, which was consistent with the resistant isolates of U. virens screened in 2015 (Zhou et al 2019).…”

Section: Discussionsupporting

confidence: 86%

“…In M. fructicola, a 65-bp sequence inserted at 113 bp upstream of the MfCYP51 gene was reported to be associated with DMI fungicide resistance (Chen et al 2017). In this study, the 'CC' insertion was found in three resistant isolates (2017-61, 2017-170, and 2017-179) at 154 bp upstream of the CYP51 gene as previously reported in resistant isolate 88 collected in 2015 (Zhou et al 2019). In addition, the expression levels of CYP51 and its encoded protein were significantly increased in these isolates (Fig.…”

Section: Discussionsupporting

confidence: 82%

“…Wang et al (2015) obtained DMI-resistant mutants of U. virens by UV-irradiation in the laboratory. However, two propiconazole-resistant U. virens isolates were detected in Huai'an, Jiangsu Province, in 2015 (Zhou et al 2019). In the current study, six out of 180 U. virens isolates were found to be resistant to propiconazole.…”

Section: Discussionmentioning

confidence: 99%

“…However, due to the intensive application of DMI fungicides, resistance has been detected in several important pathogens, such as Monilinia fructicola, Puccinia triticina, Penicillium digitatum, and Erysiphe graminis, in recent years (Schepers 1985;Delye et al 1998;Price et al 2015). In 2015, Zhou et al (2019) reported propiconazole-resistant isolates of U. virens and found one with high fitness. Therefore, monitoring propiconazole resistance in the field and evaluating its resistance risk are particularly important for the management of DMI resistance in RFS.…”

Section: Introductionmentioning

confidence: 99%

“…In M. fructicola, a transposable element 'Mona' was inserted in the 113 bp upstream of MfCYP51, which determined the resistance to DMI fungicides Chen et al 2017). In U. virens, up-regulation of CYP51 and increased ergosterol biosynthesis were reported to be associated with propiconazole resistance (Zhou et al 2019). However, due to the lack of resistant field isolates, the resistance mechanism of U. virens to DMI fungicides requires further investigation.…”

Section: Introductionmentioning

confidence: 99%

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Monitoring and analysis of rice pathogen Ustilaginoidea virens isolates with resistance to sterol demethylation inhibitors in China

Cao

et al. 2020

Phytopathol Res

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Rice false smut (RFS), caused by Ustilaginoidea virens (Cooke) Takah, is an important fungal disease of rice. In China, sterol demethylation inhibitors (DMIs) are common fungicides used to control RFS. In a previous study, we detected two propiconazole-resistant U. virens isolates in 2015 in Huai'an city, Jiangsu Province, China. In the current study, we detected six propiconazole-resistant isolates out of 180 U. virens isolates collected from rice fields in Jiangsu Province in 2017, and found they were from three different places (Xuzhou, Huai'an and Jintan). All these six propiconazole-resistant isolates were cross-resistant to three other sterol demethylation inhibitor (DMI) fungicides, i.e. difenoconazole, tebuconazole, and epoxiconazole. Among them, two isolates (2017-61 and 2017-170) had high fitness. Through sequencing and RT-qPCR analysis, we found that the expression levels of CYP51 and its encoded protein were significantly increased in the propiconazole-resistant isolates with a "CC" insertion mutation upstream of the CYP51 coding region compared to the propiconazole-sensitive isolates. In addition, propiconazole stimulated CYP51 expression in all isolates. Propiconazole also stimulated the accumulation of CYP51 protein in propiconazole-sensitive isolates and propiconazole-resistant isolates without mutation, but not in propiconazole-resistant isolates with the "CC" mutation. According to JASPAR database analysis, the predicated functional binding sites for propiconazole-resistant isolates with a "CC" insertion mutation and propiconazole-sensitive isolates were different. Given the high fitness of the propiconazoleresistant isolates, the development of resistance to DMIs in U. virens should be monitored. Furthermore, we speculated that the over-expression of CYP51 may contribute to DMI resistance in U. virens with the "CC" insertion mutation.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Monitoring and analysis of rice pathogen Ustilaginoidea virens isolates with resistance to sterol demethylation inhibitors in China

Cao

et al. 2020

Phytopathol Res

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Integrating a Bacillus‐based product with fungicides by foliar application to protect soybean: a sustainable approach to avoid exclusive use of chemicals

Santos

Viera

Camargo

et al. 2022

Pest Management Science

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BACKGROUND Biological control is widely applied against soybean (Glycine max L.) soil‐borne pathogens by furrow or seed treatments. The efficiency of bioproducts and timing of application by foliar sprays against soybean pathogens needs to be more fully understood. This work investigated the efficiency of foliar application with a Bacillus subtilis‐based product (BBP) to protect soybean from multiple pathogens and the best moment to apply it considering its compatibility with fungicides. RESULTS Foliar applications (from stages V6 to R4) with BBP, mancozeb (M) and systemic fungicides (S) applied against Asian rust (Phakopsora pachyrhizi), BBP followed by S (BBP‐S), BBP along with S (S + BBP), S along with M (S + M) or water were carried out in a soybean field for two years. The treatments S + BBP, S + M and BBP‐S reduced the severity of Asian rust by 82% compared to the control. Except for M, all treatments reduced the severity of other foliar diseases by >60%. The defoliation was reduced by BBP and BBP‐S, whereas the yield was 25% higher than control by using BBP, S + M, S + BBP or BBP‐S. Also, the incidence of Fusarium sp. was significantly reduced on seeds from treatments BBP and BBP‐S. The compatibility tests in Petri dishes demonstrated that all fungicides decreased the bacterial growth when applied along with BBP, whereas previous applications of BBP did not reduce the bacterial growth. CONCLUSIONS The BBP protected soybean against multiple pathogens, reduced defoliation and promoted soybean yield similarly to conventional fungicides, but in general, the early application of the BBP was the most efficient protection. © 2022 Society of Chemical Industry.

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Combination effects of tebuconazole with Bacillus subtilis to control rice false smut and the related synergistic mechanism

Liu

Zhao

Cai

et al. 2022

Pest Management Science

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Background: Management of rice false smut is based mainly on chemical control, which poses many safety and environmental challenges. The other option, biological control with biofungicides, does not have such problems but is not as reliable because of low systemic ability, and lower and unstable efficacy. Therefore, it is necessary to combine application of chemical fungicides and biological control agents (BCAs) and elucidate their synergistic mechanism.Results: A combination of tebuconazole and a proven BCA, Bacillus subtilis H158, was evaluated for control of rice false smut. Tebuconazole at low application rates stimulated growth of B. subtilis, prolonged the effective period of B. subtilis by enhancing its persistence on the surface of rice plants, accelerated biofilm formation of the BCA to facilitate colonization, promoted induced systemic resistance in rice by regulating defense-related enzymes and genes, and reduced the natural resistance of the pathogen by suppressing the key gene for fungal resistance to tebuconazole. However, at high application rates, tebuconazole had adverse effects on these factors and showed antagonistic combination effects with B. subtilis. The combination of B. subtilis with tebuconazole at low application rates showed great synergistic effects, but at high application rates showed only antagonistic effects in field experiments over two consecutive years. Conclusion:The combination of B. subtilis with tebuconazole had significant synergistic effects at low application rates. The synergistic effects are the result of multiple mechanisms involved in BCA, rice and the fungal pathogen.

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Characterization of propiconazole field-resistant isolates of Ustilaginoidea virens

Cited by 26 publications

References 85 publications

Monitoring and analysis of rice pathogen Ustilaginoidea virens isolates with resistance to sterol demethylation inhibitors in China

Monitoring and analysis of rice pathogen Ustilaginoidea virens isolates with resistance to sterol demethylation inhibitors in China

Integrating a Bacillus‐based product with fungicides by foliar application to protect soybean: a sustainable approach to avoid exclusive use of chemicals

Combination effects of tebuconazole with Bacillus subtilis to control rice false smut and the related synergistic mechanism

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