Characterization of Probiotic Jelly Drinks with Addition of Lactobacillus plantarum (SK5) from Bekasam during Storage

Abstract

AbstrakBakteri asam laktat asal bekasam selain berpotensi sebagai antimikroba dan penghasil enzim, juga sebagai probiotik. Penelitian ini bertujuan mendapatkan formula yang terpilih dari minuman jelly dengan penambahan karaginan berdasarkan uji organoleptik serta menentukan pengaruh penambahan biomassa basah (BB) dan biomassa kering (BK) Lactobacillus plantarum (SK5) asal bekasam selama penyimpanan terhadap parameter kimiawi dan mikrobiologis. Penelitian ini terdiri atas dua tahapan yaitu penelitian pendahuluan untuk menentukan konsentrasi karaginan terbaik dan penelitian utama untuk menentukan karakteristik kimiawi dan mikrobiologis minuman jelly probiotik selama penyimpanan. Konsentrasi karaginan yang digunakan yaitu 0,67%; 1% dan 1,34%. Konsentrasi karaginan terbaik yang didapat dari hasil penelitian pendahuluan yaitu 0,67%. Hasil karakterisasi BB selama penyimpanan mengalami penurunan terhadap pH sebesar 2,29; total mikroba 0,91 log cfu/g; total BAL 0,2 log cfu/g; serat pangan 1,26% dan total gula 0,53%. Karakterisasi BK selama penyimpanan mengalami penurunan terhadap pH sebesar 2,54; total mikroba 3,13 log cfu/g; total BAL 2,22 log cfu/g, dan mengalami peningkatan terhadap serat pangan sebesar 2,13% dan total gula 1,96%.Kata kunci: Karaginan, Lactobacillus plantarum (SK5), minuman jelly, probiotik Abstract Lactic acid bacteria from bekasam has potential as an antimicrobial and enzyme producer, as well as probiotics. The purpose of this study were to get the selected formula of the jelly drink with the addition of carrageenan by organoleptic and determine the effect of adding wet biomass (WB) and dry biomass (DB) Lactobacillus plantarum (SK5) from bekasam against chemical and microbiological parameters jelly beverage probiotics during storage. This study consisted of two phases where a preliminary study to determine the best carrageenan concentration and primary research to determine the chemical and microbiological characteristics jelly drink probiotics during storage. Concentrations of carrageenan used were 0.67%; 1% and 1.34%. The best concentration obtained from the preliminary study was 0.67%. The pH of WB during storage decreased to 2.29; total microbial 0.91 log cfu/g; BAL total of 0.2 log cfu/g; dietary fiber 1.26% and 0.53% total sugars. Dry biomass characterization during storage decreased to pH of 2.54; Microbial total of 3.13 log cfu/g; LAB total of 2.22 log cfu/g, while the increase of the dietary fiber of 2.13% and total sugars 1.96%.