Characterization of Primary Breast Carcinomas Grown in Three-Dimensional Cultures

Becker, Jeanne L.; Blanchard, D K

doi:10.1016/j.jss.2007.03.016

Cited by 32 publications

(20 citation statements)

References 33 publications

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“…Alternatively, similar benefits can be achieved by employing semi-permeable vessel walls over which media is perfused [203]. This approach has also been used to generate 3-D prostate, melanoma, and breast cancer cell spheroids or organoids that mimicked in vivo cancerous tissue growth [204-209], further demonstrating that fluid shear is an important microenvironmental parameter for a variety of cancers.…”

Section: Models Of Applied Mechanical Loadingmentioning

confidence: 99%

Biomechanical forces in the skeleton and their relevance to bone metastasis: Biology and engineering considerations

Lynch

Fischbach

2014

Advanced Drug Delivery Reviews

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Bone metastasis represents the leading cause of breast cancer related-deaths. However, the effect of skeleton-associated biomechanical signals on the initiation, progression, and therapy response of breast cancer bone metastasis is largely unknown. This review seeks to highlight possible functional connections between skeletal mechanical signals and breast cancer bone metastasis and their contribution to clinical outcome. It provides an introduction to the physical and biological signals underlying bone functional adaptation and discusses the modulatory roles of mechanical loading and breast cancer metastasis in this process. Following a definition of biophysical design criteria, in vitro and in vivo approaches from the fields of bone biomechanics and tissue engineering will be reviewed that may be suitable to investigate breast cancer bone metastasis as a function of varied mechano-signaling. Finally, an outlook of future opportunities and challenges associated with this newly emerging field will be provided.

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Section: Models Of Applied Mechanical Loadingmentioning

confidence: 99%

Biomechanical forces in the skeleton and their relevance to bone metastasis: Biology and engineering considerations

Lynch

Fischbach

2014

Advanced Drug Delivery Reviews

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“…In addition, in the case of chondrosarcomas, cells fail to produce their characteristic abundant hyaline extracellular matrix. Therefore, the traditional 2D cell cultures cannot ideally recapitulate in vivo physiological conditions [6, 11] . …”

Section: Introductionmentioning

confidence: 99%

Identification of an easy to use 3D culture model to investigate invasion and anticancer drug response in chondrosarcomas

et al. 2017

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BackgroundCytotoxic efficacy of anticancer drugs has been widely studied with monolayer-cultured cancer cells. However, the efficacy of drugs under two-dimensional (2D) culture condition usually differs from that of three-dimensional (3D) one. In the present study, an in vitro tumor tissue model was constructed using alginate hydrogel, and in vitro cytotoxic efficacy of two anticancer drugs (cisplatin and DZNep) was investigated in chondrosarcomas, and compared to in vivo response.MethodsThree cell lines derived from human chondrosarcomas, CH2879, JJ012 and SW1353, were embedded in alginate hydrogel. Proliferation and survival were assayed by ATP measurement using Cell Titer-Glo luminescent cell viability assay kit, and by counting viable cells in beads. Collagen and COMP expression was determined by RT-PCR. Invasion/migration was estimated by counting cells leaving alginate beads and adhering to culture dish. Then, chondrosarcoma response to cisplatin and DZNep was compared between cells cultured in monolayer or embedded in alginate, and using chondrosarcoma xenografts in nude mice.ResultsChondrosarcomas survived at least for 8 weeks, after embedment in alginate. However, only CH2879 cells could proliferate. Also, this cell line is more invasive than SW1353 and JJ012, which was coherent with the grade of their respective primary tumors. Furthermore, the expression of type II collagen was higher in chondrosarcomas cultured in 3D than in 2D. Interestingly, this 3D culture system allows to validate the absence of response of chondrosarcomas to cisplatin, and to predict the efficiency of DZNep to reduce chondrosarcoma growth in vivo.ConclusionsThis study validates alginate beads as a relevant 3D model to study cancer biology and tumor responses to biological treatments.

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“…Many forms of in vitro three dimensional (3D) tumor models have been generated to address how co-cultured cells behave compared to cells residing together in vivo [1–3]. Investigations have examined whether immortalized cell lines coaxed into a 3D environment are able to preserve natural functions when grown as homo- or heterogeneous populations [4,5].…”

Section: Introductionmentioning

confidence: 99%

The use of an in vitro 3D melanoma model to predict in vivo plasmid transfection using electroporation

Marrero

Heller

2012

Biomaterials

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A large-scale in vitro 3D tumor model was generated to evaluate gene delivery procedures in vivo. This 3D tumor model consists of a “tissue-like” spheroid that provides a micro-environment supportive of melanoma proliferation, allowing cells to behave similarly to cells in vivo. This functional spheroid measures approximately 1 cm in diameter and can be used to effectively evaluate plasmid transfection when testing various electroporation (EP) electrode applicators. In this study, we identified EP conditions that efficiently transfect green fluorescent protein (GFP) and interleukin 15 (IL-15) plasmids into tumor cells residing in the 3D construct. We found that plasmids delivered using a 6-plate electrode applying 6 pulses with nominal electric field strength of 500 V/cm and pulse-length of 20 ms produced significant increase of GFP (7.3-fold) and IL-15 (3.0–fold) expression compared to controls. This in vitro 3D model demonstrates the predictability of cellular response toward delivery techniques, limits the numbers of animals employed for transfection studies, and may facilitate future developments of clinical trials for cancer therapies in vivo.

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Characterization of Primary Breast Carcinomas Grown in Three-Dimensional Cultures

Cited by 32 publications

References 33 publications

Biomechanical forces in the skeleton and their relevance to bone metastasis: Biology and engineering considerations

Biomechanical forces in the skeleton and their relevance to bone metastasis: Biology and engineering considerations

Identification of an easy to use 3D culture model to investigate invasion and anticancer drug response in chondrosarcomas

The use of an in vitro 3D melanoma model to predict in vivo plasmid transfection using electroporation

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