Characterization of predominant bacteria from the colons of normal and dysenteric pigs

Abstract

Bacterial populations adherent to the mucosa of the proximal colons of weaned, healthy pigs were compared with populations from pigs with dysentery induced by inoculation with a culture of Treponema hyodysenteriae. Isolates (136) representative of the predominant flora adherent to colonic epithelia of normal pigs and isolates (162) from pigs with dysentery were cultured anaerobically on a rumen fluid-based medium and characterized. Most (71%) of the isolates from colonic epithelia of normal pigs were gram positive, whereas 88% of the epithelia-associated isolates from pigs with dysentery were gram negative. The geometric mean of colony counts was 5.7 x 107/cm2 of colonic tissue from three normal pigs and 7.7 x 108/cm2 from four pigs with dysentery. A number of isolates obtained from contents of the lumens of normal pigs and pigs with dysentery were also characterized. Comparison of isolates from epithelial tissue and from contents of the lumens of the same pig indicated that these populations were different. Our results indicate that physiological changes that occur in the colons of pigs with dysentery are accompanied by marked changes in the microbial populations in the colons. The factors which regulate the population changes are not yet understood.

Health‐beneficial effects of probiotics: Its mode of action

“…The majority of bacteria in the large intestine are obligate anaerobes, since the lumen in the large intestine is under an anaerobic condition. In pigs, the following genera are the predominant bacteria: Lactobacillus , Enterococcus , Ruminococcus , Clostridium , Eubacterium , Fusobacterium , Bacteroides , Prevotella , Selenomonus , Veillonella , Megasphaera , Peptostreptococcus , Acidaminococcus , Butyrivibrio , Lachnospira , and Escherichia (Robinson et al. 1981, 1984; Moore, Moore et al.…”

Taxonomical changes in intestinal (faecal) enterococci and streptococci: consequences on their use as indicators of faecal contamination in drinking water

“…T h e taxonomical position of Strep. intestinalis (Robinson et a / . 1988) as member of the genus Streptococcus has not been clarified as yet. This anaerobic species was described by Robinson et ill. (1988) as the dominant micro-organism in the proximal colon of pigs (Robinson et a/. 1984 Table 3.…”

Adhesion of Lactobacillus plantarum 423 and Lactobacillus salivarius 241 to the intestinal tract of piglets, as recorded with fluorescent in situ hybridization (FISH), and production of plantaricin 423 by cells colonized to the ileum

“…The main role of these organisms is to produce lactic acid to maintain a low stomach pH (Cranwell et al 1976;Barrow et al 1977). The most prevalent species in the porcine digestive tract are Lactobacillus acidophilus, Lactobacillus delbrückii, Lactobacillus fermentum, Lactobacillus reuteri, Lactobacillus salivarius, Enterococcus bovis, Enterococcus durans, Enterococcus faecalis, Enterococcus faecium, Streptococcus intestinalis, Strep-tococcus porcinus, Streptococcus salivarius, Bifidobacterium adolescentis and Bifidobacterium suis (Raibaud et al 1961;Zani et al 1974;Barrow et al 1977;Fuller et al 1978;Collins et al 1984;Robinson et al 1984Robinson et al ,1988. Migration of pH and bile resistant strains to the larger intestine ensures a healthy balance between probiotic strains and opportunistic pathogens (Fuller et al 1978;Dunne 2001;Ohashi et al 2004).…”