Characterization of poxB, a chromosomal-encoded Pseudomonas aeruginosa oxacillinase

Kong, Kok-Fai; Jayawardena, Suriya; Puerto, Aimee del; Wiehlmann, Lutz; Laabs, Ulrike; Tümmler, Burkhard; Mathee, Kalai

doi:10.1016/j.gene.2005.05.027

Cited by 42 publications

(48 citation statements)

References 28 publications

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“…One speculation is that E. coli PBP5 could act as a 'trap' for b-lactams thereby preventing access to their targets, the HMM PBPs whereas in P. aeruginosa loss of PBP4 results in increased production of the AmpR effector which escalates ampC b-lactamase expression and thereby the MIC [124,215,216,218] Indirect regulation of b-lactam resistance through AmpR Although P. aeruginosa AmpR acts as the nexus for AmpC b-lactamase induction and cell-wall recycling processes, it also modulates the activity of other proteins that regulate blactam resistance. One of these is the second chromosomally encoded b-lactamase, PoxB (PA5514) in P. aeruginosa [219]. PoxB is a carbapenemase, which affects b-lactam resistance, in the absence of the major b-lactamase AmpC and outer membrane porin OprD (PA0958) [220].…”

Section: Cell-wall Recycling and Antibiotic Resistancementioning

confidence: 99%

Cell-wall recycling and synthesis in Escherichia coli and Pseudomonas aeruginosa – their role in the development of resistance

Dhar

Kumari

Balasubramanian

et al. 2018

Journal of Medical Microbiology

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The bacterial cell-wall that forms a protective layer over the inner membrane is called the murein sacculus -a tightly crosslinked peptidoglycan mesh unique to bacteria. Cell-wall synthesis and recycling are critical cellular processes essential for cell growth, elongation and division. Both de novo synthesis and recycling involve an array of enzymes across all cellular compartments, namely the outer membrane, periplasm, inner membrane and cytoplasm. Due to the exclusivity of peptidoglycan in the bacterial cell-wall, these players are the target of choice for many antibacterial agents. Our current understanding of cell-wall biochemistry and biogenesis in Gram-negative organisms stems mostly from studies of Escherichia coli. An incomplete knowledge on these processes exists for the opportunistic Gram-negative pathogen, Pseudomonas aeruginosa. In this review, cell-wall synthesis and recycling in the various cellular compartments are compared and contrasted between E. coli and P. aeruginosa. Despite the fact that there is a remarkable similarity of these processes between the two bacterial species, crucial differences alter their resistance to b-lactams, fluoroquinolones and aminoglycosides. One of the common mediators underlying resistance is the amp system whose mechanism of action is closely associated with the cell-wall recycling pathway. The activation of amp genes results in expression of AmpC blactamase through its cognate regulator AmpR which further regulates multi-drug resistance. In addition, other cell-wall recycling enzymes also contribute to antibiotic resistance. This comprehensive summary of the information should spawn new ideas on how to effectively target cell-wall processes to combat the growing resistance to existing antibiotics.

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Section: Cell-wall Recycling and Antibiotic Resistancementioning

confidence: 99%

Cell-wall recycling and synthesis in Escherichia coli and Pseudomonas aeruginosa – their role in the development of resistance

Dhar

Kumari

Balasubramanian

et al. 2018

Journal of Medical Microbiology

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“…Previous work showed that ampC mutations do not completely abolish β-lactamase activity in the P. aeruginosa PA01 strain (740). Furthermore, the susceptibility profile of an ampC-deficient strain did not show an increase in sensitivity towards the three β-lactam representatives tested, benzyl-penicillin, meropenem and cefotaxime, as compared to the wild-type PA01 (740).…”

Section: The Pox Operonmentioning

confidence: 81%

“…Furthermore, the susceptibility profile of an ampC-deficient strain did not show an increase in sensitivity towards the three β-lactam representatives tested, benzyl-penicillin, meropenem and cefotaxime, as compared to the wild-type PA01 (740). In view of these results, the presence of a second chromosomally encoded β-lactamase in P. aeruginosa was postulated.…”

Section: The Pox Operonmentioning

confidence: 88%

Characterization of the poxAB Operon Encoding a Class D Carbapenemase in Pseudomonas aeruginosa,

Zincke¹

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“…This, and previous observations with P ampC -lacZ, led to the discovery of a second chromosomal ß-lactamase, an oxacillinase PoxB (106). The poxB gene is part of a twogene operon (poxAB).…”

Section: P Aeruginosa Ampr Regulates Other Genesmentioning

confidence: 86%

“…Introduction of this operon on a plasmid into E. coli, in the presence and absence of P. aeruginosa ampR, resulted in higher levels of ß-lactamase activity only in the absence of ampR. Reduction of activity in the presence of ampR suggested that AmpR negatively regulates poxB (106). Ongoing work in the Mathee lab is aimed at better understanding AmpR-regulation of poxB.…”

Section: P Aeruginosa Ampr Regulates Other Genesmentioning

confidence: 99%

Pseudomonas Aeruginosa AmpR Transcriptional Regulatory Network

Balasubramanian¹

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Characterization of poxB, a chromosomal-encoded Pseudomonas aeruginosa oxacillinase

Cited by 42 publications

References 28 publications

Cell-wall recycling and synthesis in Escherichia coli and Pseudomonas aeruginosa – their role in the development of resistance

Cell-wall recycling and synthesis in Escherichia coli and Pseudomonas aeruginosa – their role in the development of resistance

Characterization of the poxAB Operon Encoding a Class D Carbapenemase in Pseudomonas aeruginosa,

Pseudomonas Aeruginosa AmpR Transcriptional Regulatory Network

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