Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers

Popović, Mina; Stojanović, Marijana; Veličković, Zlate; Kovačević, Ana; Miljković, Radmila; Mirković, Nemanja; Marinković, Аleksandar

doi:10.1016/j.ijbiomac.2021.04.177

Cited by 16 publications

(8 citation statements)

References 54 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…When bacteria are mixed with alginate the bacteria are trapped within the polymerized matrix following the introduction of calcium. This technique has been used to protect probiotics for passage through acidic environments of the gastrointestinal track [18][19][20][21]. Lactobacillus spp.…”

Section: Introductionmentioning

confidence: 99%

Alginate microencapsulation of an attenuated O-antigen mutant of Francisella tularensis LVS as a model for a vaccine delivery vehicle

et al. 2022

View full text Add to dashboard Cite

Francisella tularensis is the etiologic agent of tularemia and a Tier I Select Agent. Subspecies tularensis (Type A) is the most virulent of the four subspecies and inhalation of as few as 10 cells can cause severe disease in humans. Due to its niche as a facultative intracellular pathogen, a successful tularemia vaccine must induce a robust cellular immune response, which is best achieved by a live, attenuated strain. F. tularensis strains lacking lipopolysaccharide (LPS) O-antigen are highly attenuated, but do not persist in the host long enough to induce protective immunity. Increasing the persistence of an O-antigen mutant may help stimulate protective immunity. Alginate encapsulation is frequently used with probiotics to increase persistence of bacteria within the gastrointestinal system, and was used to encapsulate the highly attenuated LVS O-antigen mutant WbtIG191V. Encapsulation with alginate followed by a poly-L-lysine/alginate coating increased survival of WbtIG191V in complement-active serum. In addition, BALB/c mice immunized intraperitoneally with encapsulated WbtIG191V combined with purified LPS survived longer than mock-immunized mice following intranasal challenge. Alginate encapsulation of the bacteria also increased antibody titers compared to non-encapsulated bacteria. These data suggest that alginate encapsulation provides a slow-release vehicle for bacterial deposits, as evidenced by the increased antibody titer and increased persistence in serum compared to freely suspended cells. Survival of mice against high-dose intranasal challenge with the LVS wildtype was similar between mice immunized within alginate capsules or with LVS, possibly due to the low number of animals used, but bacterial loads in the liver and spleen were the lowest in mice immunized with WbtIG191V and LPS in beads. However, an analysis of the immune response of surviving mice indicated that those vaccinated with the alginate vehicle upregulated cell-mediated immune pathways to a lesser extent than LVS-vaccinated mice. In summary, this vehicle, as formulated, may be more effective for pathogens that require predominately antibody-mediated immunity.

show abstract

Section: Introductionmentioning

confidence: 99%

Alginate microencapsulation of an attenuated O-antigen mutant of Francisella tularensis LVS as a model for a vaccine delivery vehicle

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Besides, it reduces the production of proinflammatory cytosines, improves the immune system, and promotes regulatory T cells (Popović et al, 2021). La.…”

Section: Resultsmentioning

confidence: 99%

“…(CN 10µg) and tetracycline (TE 30µg), and Li. monocytogenes was sensitive to tetracycline (TE 30 µg) and cefquinome (CEQ 30µg) Popović et al (2021). submitted a strain of La.…”

mentioning

confidence: 99%

Effect of microencapsulated Lactobacillus reuteri under simulated gastric conditions and its inhibition on Listeria monocytogenes

Jurado-Gámez,

Cerón-Córdoba,

Bolaños-Bolaños

2023

rcia

View full text Add to dashboard Cite

Food-borne diseases (FDB) are responsible for causing approximately 600 million illnesses and 420,000 deaths per year. Biologically related FBDs are typically associated with ubiquitous microorganisms, with bacteria such as Li. monocytogenes, Escherichia coli, Salmonella and Staphylococcus aureus being frequently implicated. The use of probiotics is limited by adverse conditions, that can impair the stability of La. reuteri and the evaluation of its probiotic properties and effects on pathogenic bacteria. Therefore, it is crucial to develop effective strategies to protect probiotics during their use. This study was conducted aiming to determine the effect of microencapsulation by spray drying technique on the probiotic viability of La. reuteri on Li. monocytogenes under simulated gastric conditions. The research involved reconstituting, planting, and inoculating La. reuteri and Li. monocytogenes; determining fermentation kinetics; conducting spray drying microencapsulation; studying and characterizing of microencapsulation; testing for exopolysaccharides production; conducting temperature tests; assessing exposure to gastric conditions; and conducting antibiotic susceptibility and inhibition tests. Such investigations allowed the establishment of the exponential phase in Probiotic (PRO) culture medium at 18 h and in De Man, Rogosa and Sharpe agar (MRS) medium at 12 h, exopolysaccharide production positive and growth at different temperatures (1.95x1013 CFU/ml and 2.16x1012 CFU/ml), survival against gastric conditions (greater than 108 CFU/ml) and inhibitory effect of La. reuteri on Li. monocytogenes (halos larger than 2 mm). The probiotic La. reuteri microencapsulated in a binary matrix composed of inulin and maltodextrin expresses probiotic properties against Li. monocytogenes, which is responsible for FBD and great stability after undergoing simulated gastric conditions.

show abstract

“…oxytoca J7 je izolovana iz fecesa C57BL/6 miševa, identifikovanih sekvenciranjem 16S rDNK, prema Popović i sar. 2021 [12], i čuvan u kolekciji bakterijskih ćelija na Institutu Torlak, Beograd Srbija. Reprezentativni izolati su identifikovani korišćenjem sekvenciranja 16S rDNK, sa univerzalnim prajmerima (UNI16Sfv i UNI16Srev) [13].…”

Section: Poreklo Bakterijske Kulture I Egzopolisaharidaunclassified

Characterization and utilization of novel exopolysaccharide isolated from klebsiella oxytoca j7, in the biosorption studies of removal of Pb2+ and Ni2+ ions from wastewater

Ljubić¹

2022

Proceedings of the 35th International Congress on Process Engineering

View full text Add to dashboard Cite

Exopolysaccharides (EPS), as biopolymers, are very common in nature. These are high molecular weight biopolymers, which have applications in various industries, and as such have attracted the attention of researchers around the world in recent years. Considering this, the main advantages of biosorption are low cost, high efficiency of metal removal from dilute solution, minimization of chemical and / or biological sludge, no additional nutrients and regeneration of biosorbent, exopolysaccharides have found application in these processes. Therefore, in this study, a new biopolymer, exopolysaccharide was isolated from Klebsiella oxytoca J7 strain, and, was studied to remove nickel and lead from wastewater. After characterization by SEM, FTIR, TGA analysis, the obtained results showed that EPS was isolated from strain K. oxytoca J7, is stable, and obtain a good nickel removal efficiency, 98.9%, while the removal efficiency for lead was 89.2%. Therefore, this exopolysaccharide can find its application in the treatment of contaminated water with heavy metals.

show abstract

Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers

Cited by 16 publications

References 54 publications

Alginate microencapsulation of an attenuated O-antigen mutant of Francisella tularensis LVS as a model for a vaccine delivery vehicle

Alginate microencapsulation of an attenuated O-antigen mutant of Francisella tularensis LVS as a model for a vaccine delivery vehicle

Effect of microencapsulated Lactobacillus reuteri under simulated gastric conditions and its inhibition on Listeria monocytogenes

Characterization and utilization of novel exopolysaccharide isolated from klebsiella oxytoca j7, in the biosorption studies of removal of Pb2+ and Ni2+ ions from wastewater

Contact Info

Product

Resources

About