Characterization of potato virus Y isolates from tomato crops in northeast Spain

Abstract

A collection of 39 potato virus Y (PVY) isolates from tomato, originating from several commercial crops grown in north-east Spain, was characterised by biological, serological and molecular assays. In general, no correspondence among the three different assays was observed. The results of biological characterization by inoculation of PVY isolates to Nicotiana spp. and pepper plants did not coincide with the differentiation provided by enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies specific to PVY C , PVY O/C or PVY N strains. In some cases this was a consequence of mixed infection of different PVY strains, which selectively infected the different hosts as demonstrated by the ELISA test. Nevertheless, the majority of PVY isolates collected from the same tomato fields showed a high degree of similarity, which indicated the predominance of one-source of infection for each tomato field. Comparison of isolates based on genetic studies did not correspond well with pathological properties, because amplification by reverse transcriptase polymerase chain reaction (RT-PCR) was also selective for some PVY isolates. The sequence analysis of some PVY isolates revealed three main clusters corresponding to NTN and N potato-type strain and NP (non potato-type) strains. Tomato seems to be slightly selective with respect to PVY infection and it is possible to find a great genetic diversity of PVY isolates belonging to different groups, mixtures and probably recombinants, which can complicate the adoption of cultural measures and the development of resistance programmes to control the disease.

Simultaneous detection of six RNA plant viruses affecting tomato crops using a single digoxigenin-labelled polyprobe

“…Thus, samples 80.11 and 80.12 tested positive for PMoV, which could not be analysed by ELISA since no antiserum was available, whereas samples 209.5, 210.3, 211.2 and 211.3 rendered positive for PVY. Much genetic and serological diversity of PVY isolates infecting tomato in Spain has been reported (Aramburu et al 2006). The results of this work showed that some PVY isolates from tomato only provided a weak positive reaction when the ELISA test was achieved using polyclonal antiserum, and some of them were not detected when specific monoclonal antibodies were used.…”

Extreme resistance to Potato virus Y in potato carrying the Rysto gene is mediated by a TIR‐NLR immune receptor

“…Potato virus Y (PVY), a member of the Potyvirus genus (Potyviridae family), is the most economically important virus of potato crops, affecting tuber yield and quality and resulting in losses of 10–90% depending on the year, cultivar and location (De Bokx and der van Want, ; Valkonen, ). PVY can also infect other Solanaceae species such as bell pepper, tomato and tobacco (Aramburu et al ., ). PVY is mechanically transmitted by more than 40 aphid species in a non‐persistent manner (Brunt, ).…”

Grafting alters tomato transcriptome and enhances tolerance to an airborne virus infection

“…Biological classification of PVY strains is based mostly on the host from which they were isolated 26 . Strong strain-host specificity has been observed in potato and pepper 27 whereas tomato seems poorly selective with respect to symptoms induced by different PVY isolates [27][28][29][30][31][32] . Tomato plants infected by PVY O or PVY C strains show crinkling of young leaves often followed by necrotic mottling and necrosis of the veins on the lower leaf surface while fruits remain usually symptomless 33 .…”