Characterization of Potato spindle tuber viroid (PSTVd) incidence and new variants from ornamentals

Matoušek, Jaroslav; Piernikarczyk, Rajen J. J.; Dědič, P.; Mertelik, J.; Uhlířová, Kateřina; Duraisamy, G; Orctová, Lidmila; Kloudova, K.; Ptáček, Jiří; Steger, Gerhard

doi:10.1007/s10658-013-0304-6

Cited by 19 publications

(6 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Differences between amino acid sequences in the 7K and 11K proteins might also determine biological properties of PVS strains [9,11,20]. In this study, amino acid analysis based on previous analysis of the 11K protein of PVS isolates [11] revealed that PVS A isolates shared amino acids glutamate at positions 4 and 7, tyrosine at 14, isoleucine at 19 and aspartate at 23.…”

Section: Resultsmentioning

confidence: 60%

“…We analyzed the frequency of pairwise sequence identities of the 72 PVS CP gene sequences at 1% interval (Supplementary Figure 1; 1628 could be divided into 15 genotypes and PVS A into 3 genotypes ( Fig. 3; Table 3 [11,20], two major blocks of 11 and 8 amino acids in the CP and 11K proteins, respectively, were not homologous and may determine biological differences between PVS isolates.…”

Section: Resultsmentioning

confidence: 99%

“…PVS virions are filamentous particles with dimensions of 610-710 9 10 -15 nm [17,18]. The viral RNA is encapsidated with a 34 kDa coat protein (CP) [10,22] and the full length RNA genome is *8.5 kb [18,21]. The RNA genome contains a 5 0 -cap structure, six open reading frames (ORFs), and a poly-A tail at the 3 0 -terminus.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of Iranian Potato virus S isolates

et al. 2011

View full text Add to dashboard Cite

Two hundred forty potato samples with one or more symptoms of leaf mosaic, distortion, mottling and yellowing were collected between 2005 and 2008 from seven Iranian provinces. Forty-four of these samples tested positive with double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) using a Potato virus S (PVS) polyclonal antibody. Of these 12 isolates of PVS were selected based on the geographical location for biological and molecular characterization. The full coat protein (CP) and 11K genes from 12 PVS isolates were PCR amplified, cloned and sequenced. All 12 PVS isolates showed mosaic symptoms on Nicotiana debneyii and N. tabacum cv. Whiteburly and local lesion on Chenopodium amaranticolor, C. quinoa and C. album. The Iranian isolates share between 93 and 100% pairwise nucleotide identity with other PVS(O) isolates. Based on maximum likelihood phylogenetic analysis coupled with pairwise identity analysis, we propose 15 genotypes for the PVS(O) strain and 3 genotypes for the PVS(A) strain.

show abstract

Section: Resultsmentioning

confidence: 60%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Iranian Potato virus S isolates

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Most of the viroid-susceptible plant species in this study were asymptomatic; therefore, they may act as carriers for the undetected spread of viroids to important crops, such as potato and tomato, through contaminated seeds or vegetatively propagated plants (Verhoeven et al 2010a;Matoušek et al 2014). Recently, PSTVd was isolated from Dahlia spp., which had been not identified as a host plant of PSTVd in Japan (Tsushima et al 2011) and is asymptomatic.…”

mentioning

confidence: 94%

Host ranges of Potato spindle tuber viroid, Tomato chlorotic dwarf viroid, Tomato apical stunt viroid, and Columnea latent viroid in horticultural plants

Matsushita

Tsuda

2014

Eur J Plant Pathol

View full text Add to dashboard Cite

Host ranges of Potato spindle tuber viroid (PSTVd), Tomato chlorotic dwarf viroid (TCDVd), Tomato apical stunt viroid (TASVd), and Colmunea latent viroid (CLVd) were investigated in 30 species from 12 genera of horticultural plants that are frequently traded internationally. They were mechanically inoculated, and viroid infection was checked using both ways of reverse-transcription polymerase chain reaction and back-inoculation to tomato. Among 30 species examined, eight to 10 were susceptible to PSTVd, TCDVd, TASVd, and CLVd. Most of these species belong to the Compositae and Solanaceae families, and symptoms were only observed in Calendula officinalis, Petunia× hybrida, and Solanum melongena. These data indicate a risk of unexpected viroid expansion by international trading of vegetatively propagated asymptomatic plant materials.

show abstract

“…Some of these hosts can act as reservoirs for potential future infections, imposing selective evolutionary pressure. The most recent example is the Potato Spindle Tuber Viroid case (PSTVd-family: Pospiviroidae) which was found mostly in asymptomatic ornamental plants (Solanaceae, Scrophulariaceae and Asteracae families) and nevertheless was able to infect tomato and potato [22,23]. However, viroid adaptation in these hosts created minor sequence or structural changes that leaded to dramatic effects on symptom expression [24e27].…”

Section: -General Aspectsmentioning

confidence: 99%