Characterization of Porous Materials by Fluorescence Correlation Spectroscopy Super-resolution Optical Fluctuation Imaging

Kisley, Lydia; Brunetti, Rachel M.; Tauzin, Lawrence J.; Shuang, Bo; Yi, Xiyu; Kirkeminde, Alec; Higgins, Daniel A.; Weiss, Shimon; Landes, Christy F.

doi:10.1021/acsnano.5b03430

Cited by 81 publications

(128 citation statements)

References 66 publications

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“…Some confirmation can be found in the observations made in ref. 23, where non-blinking beads were used for SOFI imaging. This led to SOFI images in which the observed structure exhibits clear distortions.…”

Section: Diffusion Causes Negligible Distortion Of the Sofi Imagesmentioning

confidence: 99%

“…For localization microscopy some initial work has been conducted on this topic22. While in the past an approach based on SOFI was used to estimate diffusion rates in real samples23, no in-depth analysis of the imaging fidelity has been performed until now. In this contribution we present a detailed investigation of the effect of fluorophore diffusion on the accuracy of SOFI imaging.…”

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confidence: 99%

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Effect of probe diffusion on the SOFI imaging accuracy

Vandenberg

Dedecker

2017

Sci Rep

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Live-cell super-resolution fluorescence imaging is becoming commonplace for exploring biological systems, though sample dynamics can affect the imaging quality. In this work we evaluate the effect of probe diffusion on super-resolution optical fluctuation imaging (SOFI), using a theoretical model and numerical simulations based on the imaging of live cells labelled with photochromic fluorescent proteins. We find that, over a range of physiological conditions, fluorophore diffusion results in a change in the amplitude of the SOFI signal. The magnitude of this change is approximately proportional to the on-time ratio of the fluorophores. However, for photochromic fluorescent proteins this effect is unlikely to present a significant distortion in practical experiments in biological systems. Due to this lack of distortions, probe diffusion strongly enhances the SOFI imaging by avoiding spatial undersampling caused by the limited labeling density.

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Section: Diffusion Causes Negligible Distortion Of the Sofi Imagesmentioning

confidence: 99%

mentioning

confidence: 99%

Effect of probe diffusion on the SOFI imaging accuracy

Vandenberg

Dedecker

2017

Sci Rep

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“…16 Unfortunately, FRAP is best suited to investigations of diffusion over long distances (i.e., 410 mm) and is thus challenging to implement in studies of single relatively-short nanotubes. 19 Such experiments are usually performed using fast frame-rate electron-multiplying CCD (EM-CCD) cameras. 18 Although it offers high temporal and spatial resolution, it is not well suited for imaging of the diffusion dynamics in heterogeneous materials.…”

Section: Introductionmentioning

confidence: 99%

Imaging fluorescence correlation spectroscopy studies of dye diffusion in self-assembled organic nanotubes

Nagasaka

Kameta

et al. 2016

Phys. Chem. Chem. Phys.

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The rate and mechanism of diffusion by anionic sulforhodamine B (SRB) dye molecules within organic nanotubes self-assembled from bolaamphiphile surfactants were investigated by imaging fluorescence correlation spectroscopy (imaging-FCS). The inner and outer surfaces of the nanotubes are terminated with amine and glucose groups, respectively; the former allow for pH-dependent manipulation of nanotube surface charge while the latter enhance their biocompatibility. Wide-field fluorescence video microscopy was used to locate and image dye-doped nanotubes dispersed on a glass surface. Imaging-FCS was then used to spatially resolve the SRB transport dynamics. Mobilization of the dye molecules was achieved by immersion of the nanotubes in buffer solution. Experiments were performed in pH 6.4, 7.4 and 8.4 buffers, at ionic strengths ranging from 1.73 mM to 520 mM. The results show that coulombic interactions between cationic ammonium ions on the inner nanotube surface and the anionic SRB molecules play a critical role in governing mass transport of the dye. The apparent dye diffusion coefficient, D, was found to generally increase with increasing ionic strength and with increasing pH. The D values obtained were found to be invariant along the nanotube length. Mass transport of the SRB molecules within the nanotubes is concluded to occur by a desorption-mediated Fickian diffusion mechanism in which dye motion is slowed by its coulombic interactions with the inner surfaces of the nanotubes. The results of these studies afford information essential to the use of organic nanotubes in controlled drug release applications.

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“…48 Besides the promising applications of SOFI microscopy in biological imaging, Roeffaers and Weckhuysen first reported the implementation of SOFI microscopy for quantitative investigations of individual catalytic activities associated with the sub-micrometer zeolite ZSM-5 in real-life fluid catalytic cracking (FCC) processes. 50 Undoubtedly, a significant resolution enhancement has been achieved with single-molecular localization-based super-resolution imaging techniques; however, their feasibility for live-cell imaging applications is confined due to restrictions of switched-on fluorophores being in close proximity, hence resulting in limited labeling density. 24).…”

Section: View Article Onlinementioning

confidence: 99%

Super-resolution fluorescent materials: an insight into design and bioimaging applications

Yang

Sharma²,

et al. 2016

Chem. Soc. Rev.

203

132

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Living organisms are generally composed of complex cellular processes which persist only within their native environments. To enhance our understanding of the biological processes lying within complex milieus, various techniques have been developed. Specifically, the emergence of super-resolution microscopy has generated a renaissance in cell biology by redefining the existing dogma towards nanoscale cell dynamics, single synaptic vesicles, and other complex bioprocesses by overcoming the diffraction-imposed resolution barrier that is associated with conventional microscopy techniques. Besides the typical technical reliance on the optical framework and computational algorithm, super-resolution imaging microscopy resorts largely to fluorescent materials with special photophysical properties, including fluorescent proteins, organic fluorophores and nanomaterials. In this tutorial review article, with the emphasis on cell biology, we summarize the recent developments in fluorescent materials being utilized in various super-resolution techniques with successful integration into bio-imaging applications. Fluorescent proteins (FP) applied in super-resolution microscopy will not be covered herein as it has already been well summarized; additionally, we demonstrate the breadth of opportunities offered from a future perspective.

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Characterization of Porous Materials by Fluorescence Correlation Spectroscopy Super-resolution Optical Fluctuation Imaging

Cited by 81 publications

References 66 publications

Effect of probe diffusion on the SOFI imaging accuracy

Effect of probe diffusion on the SOFI imaging accuracy

Imaging fluorescence correlation spectroscopy studies of dye diffusion in self-assembled organic nanotubes

Super-resolution fluorescent materials: an insight into design and bioimaging applications

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