Characterization of Pore Structure in Biologically Functional Poly(2-Hydroxyethyl Methacrylate) - Poly(Ethylene Glycol) Diacrylate (PHEMA-PEGDA)

Abstract

A copolymer composed of poly(2-hydroxyethyl methacrylate) (PHEMA) and poly(ethylene glycol) diacrylate (PEGDA) (PHEMA-PEGDA) is structurally versatile. Its structure can be adjusted using the following porogens: water, sucrose, and benzyl alcohol. Using phase separation technique, a variety of surface architectures and pore morphologies were developed by adjusting porogen volume and type. The water and sucrose porogens were effective in creating porous and cytocompatible PHEMA-PEGDA scaffolds. When coated with collagen, the PHEMA-PEGDA scaffolds accommodated cell migration. The PHEMA-PEGDA scaffolds are easy to produce, non-toxic, and mechanically stable enough to resist fracture during routine handling. The PHEMA-PEGDA structures presented in this study may expedite the current research effort to engineer tissue scaffolds that provide both structural stability and biological activity.

A Hydrogel Vitreous Substitute that Releases Antioxidant

“…The cells cultured in the hydrogel extracts were not statistically different than the control cells cultured in normal culture media. This result is unsurprising since PEGDA and PEGMA, being PEG derivatives, have been shown to be biocompatible in previous studies for ophthalmic applications . ARPE‐19 cells appeared to not be as sensitive to hydrogen peroxide as LECs.…”

“…Multiple formulations of HEMA, PEGMA, and PEGDA copolymers were screened at the start of this study. HEMA, PEGMA, and PEGDA hydrogels have been previously used in ocular biomaterials such as vitreous substitutes, contact lenses, or corneal prostheses, and therefore were selected as preliminary polymers for this study . Copolymerization with HEMA at low polymer concentration (less than 3%) was found to result in collapsed opaque hydrogels; accordingly HEMA was removed as a potential candidate for vitreous substitutes.…”

Tailoring of the porous structure of soft emulsion-templated polymer materials

“…Gas-filled porous materials have unique thermal, mechanical, acoustical and transport properties that may be tuned by varying either the matrix composition or the porous structure. 1 Porous materials with mineral, plastic, metal-organic, carbon matrices are widely used in various applications such as thermal isolation, hydrogen storage, shock wave absorption, catalysis, membrane filtrations, medical implants and tissue engineering 2,3,4,5,6 . Due to the presence of large proportion of gas, porous materials also present a high compressibility, which makes them interesting for acoustic applications.…”

The thickness of poly‐phenoxyethyl methacrylate brush interferes with cellular behavior and function of myofibers

“…The PHEMA brush was reported to be very stable, without decomposition or decrement in film thickness in phosphate‐buffered saline (PBS) for 20 days . Excellent antifouling ability and high structural stability make PHEMA the promising biomaterials for biomedical applications . Recently, we polymerized hydroxyethyl methacrylate (PHEMA) on a glass substrate via the surface‐initiated atomic transfer radical polymerization (SI‐ATRP) method, allowing us to control the thickness and composition of the polymer brush accurately .…”

“…19 Excellent antifouling ability and high structural stability make PHEMA the promising biomaterials for biomedical applications. [19][20][21] Recently, we polymerized hydroxyethyl methacrylate (PHEMA) on a glass substrate via the surface-initiated atomic transfer radical polymerization (SI-ATRP) method, allowing us to control the thickness and composition of the polymer brush accurately. 22 To study whether grafting thickness of biomimetic layer on the substrate leads to the myoblasts/myofiber behavior change, this study explores muscle cell response on PHEMA brush with different thicknesses, which would be valuable for the design and preparation of the modified layer on muscle tissue engineering scaffolds.…”