Characterization of porcine Trueperella pyogenes by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), molecular typing and antimicrobial susceptibility profiling in Sao Paulo State

Moreno, Luisa Zanolli; Matajira, Carlos Emílio Cabrera; Costa, Bruno Ruiz Brandão da; Ferreira, Tiago Osório; Silva, Givago F. R.; Dutra, Maurício Cabral; Gomes, Vasco T.M.; Silva, Ana Paula S.; Christ, Ana Paula Guarnieri; Sato, Maria Inês Z.; Moreno, Andréa Micke

doi:10.1016/j.cimid.2017.03.005

Cited by 16 publications

(23 citation statements)

References 20 publications

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“…Sometimes, additional bacteriological methods other than the conventional ones are necessary for the differentiation and appropriate identification of isolates. New techniques, such as loop-mediated isothermal amplification (LAMP) assay, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, Fourier transform infrared (FT-IR) spectroscopy, or 16S rRNA gene sequencing may be useful for the diagnostics of T. pyogenes infections [8,9,10,11,12,13]. Those methods enable the recognition of the closely related taxa of the order Actinomycetales , and sometimes the reclassification of some of them [14].…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity and Virulence of Trueperella pyogenes: A Review

Rzewuska

Kwiecień

Chrobak-Chmiel

et al. 2019

IJMS

122

110

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Bacteria from the species Trueperella pyogenes are a part of the biota of skin and mucous membranes of the upper respiratory, gastrointestinal, or urogenital tracts of animals, but also, opportunistic pathogens. T. pyogenes causes a variety of purulent infections, such as metritis, mastitis, pneumonia, and abscesses, which, in livestock breeding, generate significant economic losses. Although this species has been known for a long time, many questions concerning the mechanisms of infection pathogenesis, as well as reservoirs and routes of transmission of bacteria, remain poorly understood. Pyolysin is a major known virulence factor of T. pyogenes that belongs to the family of cholesterol-dependent cytolysins. Its cytolytic activity is associated with transmembrane pore formation. Other putative virulence factors, including neuraminidases, extracellular matrix-binding proteins, fimbriae, and biofilm formation ability, contribute to the adhesion and colonization of the host tissues. However, data about the pathogen–host interactions that may be involved in the development of T. pyogenes infection are still limited. The aim of this review is to present the current knowledge about the pathogenic potential and virulence of T. pyogenes.

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Section: Introductionmentioning

confidence: 99%

Pathogenicity and Virulence of Trueperella pyogenes: A Review

Rzewuska

Kwiecień

Chrobak-Chmiel

et al. 2019

IJMS

122

110

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“…These log score values confirm the identification at the species level. MALDI-TOF MS for Trueperella differentiation with a log score around 2 shows comparable discriminating power with molecular methods; it is a rapid and accurate tool for T. pyogenes diagnosis [ 13 ]. Partial 16S rRNA sequencing confirmed the isolates to be T. pyogenes and cultivation on Loeffler serum revealed the typical pitting of the serum slope.…”

Section: Resultsmentioning

confidence: 99%

Trueperella pyogenesandBrucella abortusCoinfection in a Dog and a Cat on a Dairy Farm in Egypt with Recurrent Cases of Mastitis and Abortion

Wareth

El-Diasty²,

Melzer

et al. 2018

Veterinary Medicine International

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Trueperella pyogenes was isolated from a dog and a cat with a mixed infection with Brucella abortus. Both lived on a dairy cattle farm with a history of regular cases of abortion and mastitis. Identification of the bacteria was done by means of MALDI-TOF MS, loop-mediated isothermal amplification (LAMP) based on cpn60, partial 16S rRNA sequencing, and growth on Loeffler Serum Medium. Isolation of Trueperella pyogenes on the dairy farm highlights its neglected role in reproduction failure and draws attention to its effects in the dairy industry in Egypt. Diagnosis and control of abortion in Egypt should include Trueperella pyogenes as one of possible causes of abortion.

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“…Resistance to gentamicin was noted only in 7.3% of bovine isolates [ 4 ]. On the other hand, all T. pyogenes swine isolates studied in Brazil were susceptible to gentamicin and streptomycin, whereas 13.3% of them were resistant to neomycin [ 38 ]. In contrast, the prevalence of aminoglycoside resistance in T. pyogenes strains isolated from wild animals seems to be significantly lower than in livestock isolates.…”

Section: Discussionmentioning

confidence: 99%

New Determinants of Aminoglycoside Resistance and Their Association with the Class 1 Integron Gene Cassettes in Trueperella pyogenes

Kwiecień

Stefańska

Chrobak-Chmiel

et al. 2020

IJMS

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Trueperella pyogenes is an important opportunistic animal pathogen. Different antimicrobials, including aminoglycosides, are used to treat T. pyogenes infections. The aim of the present study was to evaluate aminoglycoside susceptibility and to detect aminoglycoside resistance determinants in 86 T. pyogenes isolates of different origin. Minimum inhibitory concentration of gentamicin, streptomycin, and kanamycin was determined using a standard broth microdilution method. Genetic elements associated with aminoglycoside resistance were investigated by PCR and DNA sequencing. All studied isolates were susceptible to gentamicin, but 32.6% and 11.6% of them were classified as resistant to streptomycin and kanamycin, respectively. A total of 30 (34.9%) isolates contained class 1 integrons. Class 1 integron gene cassettes carrying aminoglycoside resistance genes, aadA11 and aadA9, were found in seven and two isolates, respectively. Additionally, the aadA9 gene found in six isolates was not associated with mobile genetic elements. Moreover, other, not carried by gene cassettes, aminoglycoside resistance genes, strA-strB and aph(3’)-IIIa, were also detected. Most importantly, this is the first description of all reported genes in T. pyogenes. Nevertheless, the relevance of the resistance phenotype to genotype was not perfectly matched in 14 isolates. Therefore, further investigations are needed to fully explain aminoglycoside resistance mechanisms in T. pyogenes.

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Characterization of porcine Trueperella pyogenes by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), molecular typing and antimicrobial susceptibility profiling in Sao Paulo State

Cited by 16 publications

References 20 publications

Pathogenicity and Virulence of Trueperella pyogenes: A Review

Pathogenicity and Virulence of Trueperella pyogenes: A Review

Trueperella pyogenesandBrucella abortusCoinfection in a Dog and a Cat on a Dairy Farm in Egypt with Recurrent Cases of Mastitis and Abortion

New Determinants of Aminoglycoside Resistance and Their Association with the Class 1 Integron Gene Cassettes in Trueperella pyogenes

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