Characterization of porcine SKIP gene in skeletal muscle development: Polymorphisms, association analysis, expression and regulation of cell growth in C2C12 cells

Xiong, Qi; Chai, Jin Choul; Deng, Chang-Yan; Jiang, Siwen; Liu, Yang; Huang, Tao; Suo, Xiaojun; Zhang, Nian; Li, Xiaofeng; Yang, Qianping; Chen, Mingxin; Zheng, Rong

doi:10.1016/j.meatsci.2012.05.016

Cited by 6 publications

(5 citation statements)

References 33 publications

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“…C2C12 cell lines usually serve as an easy-to-handle model system for investigating the molecular basis of skeletal muscle cell specification and development both in humans and farm animals [ 16 , 21 , 22 ]…”

Section: Resultsmentioning

confidence: 99%

“…The C2C12 (mouse) and HEK 293T cell lines (human) used in this study were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). C2C12 cells were used to explore gene function in livestock muscle cells [ 15 , 16 ]. C2C12 cells were first cultured in growth medium (GM)—Dulbecco’s modified Eagle’s medium (DMEM, Hyclone, Logan, UT, USA)—supplemented with 10% (volume fraction) fetal bovine serum and 1% penicillin-streptomycin (Gibco, New York, NY, USA).…”

Section: Methodsmentioning

confidence: 99%

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A Specific microRNA Targets an Elongase of Very Long Chain Fatty Acids to Regulate Fatty Acid Composition and Mitochondrial Morphology of Skeletal Muscle Cells

Wang

Shen

et al. 2022

Animals

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Recently, miR-22 has been suggested to be an important microRNA (miRNA) affecting meat quality. Studies have shown that muscle fatty acid composition and mitochondrial function are closely related to meat quality. The regulatory mechanism of miR-22 on skeletal muscle fatty acid composition and mitochondrial function is not well characterized. Therefore, we aimed to explore the effects of miR-22 on fatty acid composition and mitochondrial function in C2C12 cells. Here, it demonstrate that elevated expression of miR-22 significantly repressed fatty acid elongation and mitochondrial morphology in C2C12 myoblasts, while the knockdown of miR-22 showed opposite results. Furthermore, miR-22 targets the elongase of very long chain fatty acids 6 (ELOVL6) and represses its expression in muscle cells. Knockdown of ELOVL6 mimicked the effect of miR-22 on fatty acid composition and mitochondrial function, while overexpression of ELOVL6 restored the effects of miR-22. These findings indicate that miR-22 downregulates the elongation of fatty acids and mitochondrial morphology by inhibiting ELOVL6 expression in muscle cells, which may provide some useful information for controlling muscle lipid accumulation and mitochondrial function in livestock in the future.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A Specific microRNA Targets an Elongase of Very Long Chain Fatty Acids to Regulate Fatty Acid Composition and Mitochondrial Morphology of Skeletal Muscle Cells

Wang

Shen

et al. 2022

Animals

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“…Finally, all 4 INPP5K mutant proteins tested, including p.Ile363Thr in the SKICH domain, bound to GRP78 in a similar way to control INPP5K (Wiessner et al, 2017). In connection with the congenital muscular dystrophy observed in human INPP5K mutants, it is important to note that 3 single nucleotide polymorphisms (SNPs) located in intron 1 and 6 as well as in exon 12 of the porcine INPP5K gene have been associated with some meat traits, including muscle fiber number (Xiong et al, 2011(Xiong et al, , 2012. Furthermore, INPP5K gene-modified Pps Brdm1/+ heterozygous mice had higher amounts of quadriceps muscle compared with wild-type mice (Ijuin et al, 2008).…”

Section: Inpp5k Mutations Are Responsible For a Rare Form Of Autosomamentioning

confidence: 98%

The phosphoinositide 5-phosphatase INPP5K: From gene structure to in vivo functions

Schurmans

Catsyne

Desmet

et al. 2021

Advances in Biological Regulation

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INPP5K (Inositol Polyphosphate 5-Phosphatase K, or SKIP (for Skeletal muscle and Kidney enriched Inositol Phosphatase) is a member of the phosphoinositide 5-phosphatases family. Its protein structure is comprised of a N-terminal catalytic domain which hydrolyses both PtdIns(4,5)P2 and PtdIns(3,4,5)P3, followed by a SKICH domain at the C-terminus which is responsible for protein-protein interactions and subcellular localization of INPP5K. Strikingly, INPP5K is mostly concentrated in the endoplasmic reticulum, although it is also detected at the plasma membrane, in the cytosol and the nucleus. Recently, mutations in INPP5K have been detected in patients with a rare form of autosomal recessive congenital muscular dystrophy with cataract, short stature and intellectual disability. INPP5K functions extend from control of insulin signaling, endoplasmic reticulum stress response and structural integrity, myoblast differentiation, cytoskeleton organization, cell adhesion and migration, renal osmoregulation, to cancer. The goal of this review is thus to summarize and comment recent and less recent data in the literature on INPP5K, in particular on the structure, expression, intracellular localization, interactions and functions of this specific member of the 5-phosphatases family.

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“…To this end, there is clear evidence that the use of nutrient-gene biomarkers is a crucial fingerprint for accurately elucidating the genetic and nutritional regulation of fat metabolism. Potential QTLs of complex traits and functional genes related to muscle growth, fat and IMF deposition, and many putative genes involved in the mechanism of fat distribution and marbling in pigs are becoming available [ 47 , 114 , 120 , 121 ]. Despite the far-reaching pieces of evidence from literature, the application of DNA-specific markers in simultaneously enhancing fat deposition and IMF content of pork without altering other carcass traits remains difficult to achieve.…”

Section: Qtl Regions and Snps For Fat Metabolism And Imf Accretion In Pigsmentioning

confidence: 99%

Genes Related to Fat Metabolism in Pigs and Intramuscular Fat Content of Pork: A Focus on Nutrigenetics and Nutrigenomics

Malgwi

Halas

Grünvald

et al. 2022

Animals

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Fat metabolism and intramuscular fat (IMF) are qualitative traits in pigs whose development are influenced by several genes and metabolic pathways. Nutrigenetics and nutrigenomics offer prospects in estimating nutrients required by a pig. Application of these emerging fields in nutritional science provides an opportunity for matching nutrients based on the genetic make-up of the pig for trait improvements. Today, integration of high throughput “omics” technologies into nutritional genomic research has revealed many quantitative trait loci (QTLs) and single nucleotide polymorphisms (SNPs) for the mutation(s) of key genes directly or indirectly involved in fat metabolism and IMF deposition in pigs. Nutrient–gene interaction and the underlying molecular mechanisms involved in fatty acid synthesis and marbling in pigs is difficult to unravel. While existing knowledge on QTLs and SNPs of genes related to fat metabolism and IMF development is yet to be harmonized, the scientific explanations behind the nature of the existing correlation between the nutrients, the genes and the environment remain unclear, being inconclusive or lacking precision. This paper aimed to: (1) discuss nutrigenetics, nutrigenomics and epigenetic mechanisms controlling fat metabolism and IMF accretion in pigs; (2) highlight the potentials of these concepts in pig nutritional programming and research.

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Characterization of porcine SKIP gene in skeletal muscle development: Polymorphisms, association analysis, expression and regulation of cell growth in C2C12 cells

Cited by 6 publications

References 33 publications

A Specific microRNA Targets an Elongase of Very Long Chain Fatty Acids to Regulate Fatty Acid Composition and Mitochondrial Morphology of Skeletal Muscle Cells

A Specific microRNA Targets an Elongase of Very Long Chain Fatty Acids to Regulate Fatty Acid Composition and Mitochondrial Morphology of Skeletal Muscle Cells

The phosphoinositide 5-phosphatase INPP5K: From gene structure to in vivo functions

Genes Related to Fat Metabolism in Pigs and Intramuscular Fat Content of Pork: A Focus on Nutrigenetics and Nutrigenomics

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