Characterization of polymorphic ampicillin forms

Baraldi, Cecilia; Tinti, Anna; Ottani, Stefano; Gamberini, Maria Cristina

doi:10.1016/j.jpba.2014.08.021

Cited by 41 publications

(33 citation statements)

References 21 publications

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“…There was also registered FTIR spectrum of ampicillin. The absorption peaks for this antibiotic are described in Supporting Information Table 4 .…”

Section: Resultsmentioning

confidence: 99%

“…However, the shift of peak v = 1742.3 cm −1 to v = 1744.0 cm −1 indicates the occurrence of stretching vibration originating from C═O carbonyl group. This functional group is present in the molecule of ampicillin and may be responsible for binding to the surface of the biologically synthesized silver nanoparticles probably coated with proteins, peptides or carbohydrates .…”

Section: Resultsmentioning

confidence: 99%

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Silver nanoparticles functionalized with ampicillin

et al. 2017

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The resistance of pathogenic bacteria to antibiotics has become a serious problem. The emphasis is placed on the development of new, effective antimicrobial strategies. One of them is the use of AgNPs in association with antibiotic drugs. The aim of this study was to obtain silver nanoparticles functionalized with ampicillin and to investigate the mechanism of binding antibiotics to nanoparticle using high-performance liquid chromatography approach. To confirm the occurrence of silver nanoparticles functionalization, FTIR, MALDI-TOF MS, and DLS analysis and zeta potential measurements were performed. Moreover we assessed the antibacterial activity of biologically synthesized nanoparticles functionalized with ampicillin against a range of gram (+) and gram (-) bacteria strains such as Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus epidermidis, and Escherichia coli.

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“…There was also registered FTIR spectrum of ampicillin. The absorption peaks for this antibiotic are described in Supporting Information Table 4 .…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Silver nanoparticles functionalized with ampicillin

et al. 2017

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“…The trihydrate and anhydrous form I have been the most studied forms, while the monohydrate and anhydrous form II have not received similar attention. Amorphous monohydrate and three crystalline forms, the trihydrate, the crystal form I and the crystal form II, were investigated by FT-IR and micro-Raman [29]. Also, data obtained by DSC, TGA, XRPD and hot-stage Raman spectroscopy were reported.…”

Section: Analytical Applicationsmentioning

confidence: 99%

Vibrational spectroscopy used in polymorphic analysis

Bunaciu

Aboul‐Enein

Hoang

2015

TrAC Trends in Analytical Chemistry

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“…1). The trihydrate is stable in a sealed system at temperatures up to 90 C. 32 A slow heating rate of 5 C/min will produce the anhydrate form I, whereas a faster heating rate of 60 C/min will result in anhydrate form II. If the system is heated in an open pan, the amorphous phase has been identified at temperatures above 90 C. Interestingly, it has been found that heating the trihydrate at high pressure produces a third anhydrate form of ampicillin 33 suggesting that the structural space of ampicillin is not yet fully characterized.…”

Section: Introductionmentioning

confidence: 99%

Computational Dehydration of Crystalline Hydrates Using Molecular Dynamics Simulations

Larsen

Rantanen

Johansson

2017

Journal of Pharmaceutical Sciences

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Molecular dynamics (MD) simulations have evolved to an increasingly reliable and accessible technique and are today implemented in many areas of biomedical sciences. We present a generally applicable method to study dehydration of hydrates based on MD simulations and apply this approach to the dehydration of ampicillin trihydrate. The crystallographic unit cell of the trihydrate is used to construct the simulation cell containing 216 ampicillin and 648 water molecules. This system is dehydrated by removing water molecules during a 2200 ps simulation, and depending on the computational dehydration rate, different dehydrated structures were observed. Removing all water molecules immediately and removing water relatively fast (10 water molecules/10 ps) resulted in an amorphous system, whereas relatively slow computational dehydration (3 water molecules/10 ps) resulted in a crystalline anhydrate. The structural changes could be followed in real time, and in addition, an intermediate amorphous phase was identified. The computationally identified dehydrated structure (anhydrate) was slightly different from the experimentally known anhydrate structure suggesting that the simulated computational structure could represent a kinetically trapped dehydration intermediate.

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Characterization of polymorphic ampicillin forms

Cited by 41 publications

References 21 publications

Silver nanoparticles functionalized with ampicillin

Silver nanoparticles functionalized with ampicillin

Vibrational spectroscopy used in polymorphic analysis

Computational Dehydration of Crystalline Hydrates Using Molecular Dynamics Simulations

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