Characterization of plasmid pSY3 in Sphingobium chungbukense DJ77

Abstract

This study determined the complete nucleotide sequence of the plasmid pSY3 from Sphingobium chungbukense DJ77. It was 35,735 bp long with a G+C content of 61.9%. Forty open reading frames (ORFs) were found. We predicted these ORFs would encode proteins associated with plasmid replication, conjugative transfer, transposition of genes, plasmid stability/partition, hypothetical protein, and some other functions. Genes for biodegradation were not found. No other plasmid homologous to pSY3 in the overall nucleotide sequence or gene organization could be found in the NCBI database.

Sphingomonas sediminicola Is an Endosymbiotic Bacterium Able to Induce the Formation of Root Nodules in Pea (Pisum sativum L.) and to Enhance Plant Biomass Production

“…Therefore, this extrachromosomal element could be a plasmid that we named p Ss01 . Within the Sphingomonas genus, many plasmids have been identified, and most of them have this ABC partitioning system [ 75 , 76 ] and are also large, such as p CAR3 in Sphingomonas sp. KA, p ISP0 in Sphingomonas sp.…”

Complete sequence and organization of the Sphingobium chungbukense DJ77 pSY2 plasmid

“…A genome sequencing project for S. chungbukense DJ77 (Chungbuk National University, Korea), which has two chromosomes and six plasmids, is currently in progress. While plasmid vectors are very useful tools for such genetic analyses, there is, until now, no known vector plasmid for Sphingomonadales, except for pSY3 of S. chungbukense DJ77 (Yeon and Kim, 2009) and pUT1, pUT2, and pCHQ1 of S. japonicum UT26S (Nagata et al, 2010). In this study, we present the sequence and an analysis of the pSY2 plasmid and provide insight into the plasmid-borne contribution of the host, S. chungbukense DJ77.…”