Characterization of plasma exosomal microRNAs in responding to radiotherapy of human esophageal squamous cell carcinoma

Abstract

radiotherapy is one of the main treatment methods for esophageal squamous cell carcinoma (eScc). Previous research has shown that plasma exosomal micrornas (mirnas) can predict therapeutic outcome. in the present study, to identify potential exosomal mirnas that respond to radiotherapy, plasma exosomal mirnas from eScc patients undergoing radiotherapy were isolated and sequenced. upregulated and downregulated mirnas were detected from patients pre-and post-radiotherapy, and it was found that they play distinct roles in dna damage process and endosomal mediated transport. Based on wound healing and cell counting Kit-8 assays in Te-1 human esophageal cancer cells, it was identified that representative miRNA miR-652 and miR-30a alter migration but not proliferation. The present findings identified differentially expressed miRNAs in responding to radiotherapy, and added a reference to explore non-invasive plasma biomarkers to evaluate therapeutic effects in eScc.

Impact of Radiation on Exosomes in Regulating Tumor Immune Microenvironment

“…For example, it was demonstrated that miR‑652 and miR‑30a alter migration but not proliferation. 49 These studies highlight the differentially expressed components of exosomes during and before RT and provide a reference for exploring noninvasive plasma biomarkers for diagnosis and evaluation of therapeutic effects.…”

Role of exosomal noncoding RNA in esophageal carcinoma

“…One of the adverse clinical impacts of exosomes as a source of tumors is their capacity to transfer resistance horizontally (61)(62)(63). Drug-resistant tumor cells can transfer drug resistance to sensitive cells through exosomes (18,64), thus generating new anti-tumor cell reservoirs. Exosomes from drug-resistant tumor cells may confer resistance phenotype to sensitive cells through intercellular signaling.…”

“…Many studies have found tumor-derived exosomes in the circulating blood of esophageal cancer patients (16)(17)(18). These exosomes include numerous tumor-related specific molecules, such as mRNA, protein, lipid, and non-coding RNA., making them a powerful signal transmission function (19).…”

Sequencing and validation of exosomal miRNAs panel as novel plasma biomarkers for early diagnosis and prognosis prediction in laryngeal cancer

“…Bene ting from the non-invasive, convenient, and e cient features, plasma exosome detection is increasingly explored in the early diagnosis of cancer, among which, miRNAs are more stable because of the speci c sorting mechanism [7], may offer an e cient, non-invasive or minimally invasive marker alternative. Recently plasma exosomal miRNAs have been shown to be early diagnostic markers for gastric cancer [8,9], epithelial ovarian cancer [9], pancreatic cancer [10], lung adenocarcinoma [11], and can be used to assess treatment response and prognosis in esophageal squamous cell carcinoma [12], non-small cell lung cancer [13], breast cancer [14], etc. In LSCC, serum exosomal hsa-miR-941 has been identi ed as a promising oncogenic marker, up-regulated level of hsa-miR-941 promotes cell proliferation and invasion [15].…”